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Evaluation of the In Vitro Wound-Healing Activity and Phytochemical Characterization of Propolis and Honey

2020Journal article Open access
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dc.contributor.authorAfonso, Alexandra M.
dc.contributor.authorGonçalves, Joana
dc.contributor.authorLuís, Ângelo
dc.contributor.authorGallardo, Eugenia
dc.contributor.authorDuarte, Ana P.
dc.date.accessioned2020-03-30T15:59:33Z
dc.date.available2020-03-30T15:59:33Z
dc.date.issued2020
dc.description.abstractHoney and propolis are natural substances produced by Apis mellifera that contain flavonoids, phenolic acids, and several other phytochemicals. The aim of this study was to phytochemically characterize three different types of honey and propolis, both separately and mixed, and to evaluate their wound-healing activity. Total phenolic compounds and flavonoids were determined using the Folin–Ciocalteu’s and aluminum chloride colorimetric methods, respectively. The antioxidant activity was evaluated by both the DPPH free radical scavenging assay and β-carotene bleaching test, and the anti-inflammatory activity was determined by a protein denaturation method. To evaluate the wound-healing activity of the samples, NHDF cells were subjected to a wound scratch assay. The obtained results showed that dark-brown honey presents a higher concentration of phenolic compounds and flavonoids, as well as higher antioxidant and anti-inflammatory activities. Propolis samples had the highest concentrations in bioactive compounds. Examining the microscopic images, it was possible to verify that the samples promote cell migration, demonstrating the wound-healing potential of honey and propolis.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.doi10.3390/app10051845pt_PT
dc.identifier.urihttp://hdl.handle.net/10400.6/10250
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.relationHealth Sciences Research Centre
dc.relationalterado para: “Avaliação in vitro associados ao consumo de Ayahuasca” AVALIAÇÃO IN VITRO DOS EFEITOS TOXICOLÓGICOS ASSOCIADOS AO CONSUMO DE AYAHUASCA.
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectHoneypt_PT
dc.subjectPropolispt_PT
dc.subjectPhenolic compoundspt_PT
dc.subjectAntioxidantpt_PT
dc.subjectWound-healing activitypt_PT
dc.subjectNHDF cellspt_PT
dc.titleEvaluation of the In Vitro Wound-Healing Activity and Phytochemical Characterization of Propolis and Honeypt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleHealth Sciences Research Centre
oaire.awardTitlealterado para: “Avaliação in vitro associados ao consumo de Ayahuasca” AVALIAÇÃO IN VITRO DOS EFEITOS TOXICOLÓGICOS ASSOCIADOS AO CONSUMO DE AYAHUASCA.
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F00709%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/POR_CENTRO/SFRH%2FBD%2F149360%2F2019/PT
oaire.citation.issue5pt_PT
oaire.citation.startPage1845pt_PT
oaire.citation.titleApplied Sciencespt_PT
oaire.citation.volume10pt_PT
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStreamPOR_CENTRO
person.familyNameGonçalves
person.familyNameSantos Luís
person.familyNameGallardo
person.familyNameDuarte
person.givenNameJoana
person.givenNameÂngelo Filipe
person.givenNameEugenia
person.givenNameAna Paula
person.identifier908303
person.identifier1255684
person.identifier.ciencia-id3D14-798B-65D3
person.identifier.ciencia-idF617-5CA5-EF61
person.identifier.ciencia-idBC15-D376-80FE
person.identifier.orcid0000-0001-8702-415X
person.identifier.orcid0000-0003-0712-6522
person.identifier.orcid0000-0002-1802-8998
person.identifier.orcid0000-0003-3333-5977
person.identifier.ridM-7410-2017
person.identifier.ridB-5904-2009
person.identifier.scopus-author-id24721485300
person.identifier.scopus-author-id7102602131
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublicationdfccf1f9-70d0-435b-b121-0a55b1e35be3
relation.isAuthorOfPublicationa2d7c872-99db-41e1-9bc9-82c2d47b4661
relation.isAuthorOfPublication9763ebec-c3f5-4cc9-b5df-8d3c045ed4ec
relation.isAuthorOfPublication971779ac-7c22-4f69-8bdd-4e0ec442ef2d
relation.isAuthorOfPublication.latestForDiscoverya2d7c872-99db-41e1-9bc9-82c2d47b4661
relation.isProjectOfPublicationc7cee677-067c-47b2-ab19-b2dd5b0fda9d
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