Publication
Dual-crosslinked injectable in situ forming Alginate/CaCl2/Pluronic F127/ α-Cyclodextrin hydrogels incorporating Doxorubicin and graphene-based nanomaterials for cancer chemo-photothermal therapy
| datacite.subject.fos | Ciências Médicas::Ciências da Saúde | |
| datacite.subject.sdg | 03:Saúde de Qualidade | |
| dc.contributor.author | Gonçalves, Joaquim | |
| dc.contributor.author | Melo, Bruna Daniela Lopes | |
| dc.contributor.author | Pouso, Manuel António do Rosário | |
| dc.contributor.author | Correia, Ilídio Joaquim Sobreira | |
| dc.contributor.author | de Melo-Diogo, Duarte | |
| dc.date.accessioned | 2025-10-02T14:02:42Z | |
| dc.date.available | 2025-10-02T14:02:42Z | |
| dc.date.issued | 2025-09-11 | |
| dc.description.abstract | Injectable in situ forming hydrogels have been emerging due to their capacity to perform the direct delivery of therapeutics into the tumor site with minimal off-target leakage. Particularly, physical crosslinked injectable in situ forming hydrogels are appealing due to their straightforward preparation that exploits the native jointing capabilities of specific polymers/materials. However, the features of these hydrogels (e.g., injectability, degradation, swelling) are strongly pre-determined by the physical interactions available on the selected polymers/ materials, occasionally yielding undesired outcomes. Thus, the combination of multiple physical crosslinking cues may allow the preparation of hydrogels with enhanced properties. In this work, a dual-crosslinked injectable in situ forming hydrogel was engineered by combining Pluronic F127/α-Cyclodextrin and Alginate/CaCl2 (i.e., combination of host-guest and electrostatic interactions), being loaded with Doxorubicin (chemotherapeutic drug) and Dopamine-reduced Graphene Oxide (photothermal nano-agent) for application in cancer chemophotothermal therapy. When compared to the single-crosslinked hydrogels, the dual-crosslinking contributed to the assembly of formulations with suitable injectability and improved degradation and water absorption behaviors. Moreover, the dual-crosslinked hydrogels presented a good photothermal capacity (ΔT ≈ 14 ◦C), leading to a 1.18-times enhanced Doxorubicin release. In in vitro cell-based studies, the dual-crosslinked hydrogels exhibited an excellent cytocompatibility towards healthy (normal human dermal fibroblasts) and breast cancer (MCF-7) cells. As importantly, the dual-crosslinked hydrogels were able to mediate a chemophotothermal effect that diminished the cancer cells’ viability to just 23 %. Overall, the developed dualcrosslinked injectable in situ forming hydrogels incorporating Doxorubicin and Dopamine-reduced Graphene Oxide are a promising macroscale system for breast cancer chemo-photothermal therapy. | por |
| dc.description.sponsorship | This research was funded in whole or in part by the Fundaç˜ao para a Ciˆencia e a Tecnologia, I.P. (FCT, https://ror.org/00snfqn58) under the Grants 10.54499/UIDB/00709/2020 (https://doi.org/10.544 99/UIDB/00709/2020), 10.54499/UIDP/00709/2020 (https://doi. org/10.54499/UIDP/00709/2020), PTDC/BTABTA/0696/2020, 2022.06320.PTDC (DOI 10.54499/2022.06320.PTDC), 10.54499/LA/ P/0079/2020 and 10.54499/UIDB/50022/2020. The project COMPETE2030-FEDER-00695800 is also acknowledged. | |
| dc.identifier.citation | Gonçalves, J. J., Melo, B. L., Pouso, M. R., Correia, I. J., & de Melo-Diogo, D. (2025). Dual-crosslinked injectable in situ forming Alginate/CaCl2/Pluronic F127/α-Cyclodextrin hydrogels incorporating Doxorubicin and Graphene-based nanomaterials for cancer chemo-photothermal therapy. Journal of Drug Delivery Science and Technology, 107520 | |
| dc.identifier.doi | https://doi.org/10.1016/j.jddst.2025.107520 | |
| dc.identifier.issn | 1773-2247 | |
| dc.identifier.uri | http://hdl.handle.net/10400.6/18944 | |
| dc.language.iso | eng | |
| dc.peerreviewed | yes | |
| dc.publisher | Elsevier | |
| dc.relation | Shear-thinning and self-healing hydrogels: tuning the polymer-related features for the tumor confined delivery of therapeutics aimed for metastatic breast cancer immuno-photothermal therapy | |
| dc.relation | Thiol-ene click injectable in situ forming hydrogels incorporating graphene: optimization of the polymer-related features for the immuno-photothermal therapy of metastatic breast cancer | |
| dc.relation | Bolsa de Doutoramento - Biotecnologia Médica | |
| dc.relation.hasversion | https://www.sciencedirect.com/science/article/pii/S1773224725009232?via%3Dihub | |
| dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | |
| dc.subject | Cancer | |
| dc.subject | Chemo-photothermal therapy | |
| dc.subject | Graphene family nanomaterials | |
| dc.subject | Injectable hydrogels | |
| dc.subject | Local delivery | |
| dc.subject | Macroscale delivery systems | |
| dc.title | Dual-crosslinked injectable in situ forming Alginate/CaCl2/Pluronic F127/ α-Cyclodextrin hydrogels incorporating Doxorubicin and graphene-based nanomaterials for cancer chemo-photothermal therapy | por |
| dc.type | research article | |
| dspace.entity.type | Publication | |
| oaire.awardTitle | Shear-thinning and self-healing hydrogels: tuning the polymer-related features for the tumor confined delivery of therapeutics aimed for metastatic breast cancer immuno-photothermal therapy | |
| oaire.awardTitle | Thiol-ene click injectable in situ forming hydrogels incorporating graphene: optimization of the polymer-related features for the immuno-photothermal therapy of metastatic breast cancer | |
| oaire.awardTitle | Bolsa de Doutoramento - Biotecnologia Médica | |
| oaire.awardURI | info:eu-repo/grantAgreement/FCT/CEEC IND4ed/2021.00590.CEECIND%2FCP1661%2FCT0001/PT | |
| oaire.awardURI | info:eu-repo/grantAgreement/FCT/Concurso de Projetos de I&D em Todos os Domínios Científicos - 2022/2022.06320.PTDC/PT | |
| oaire.awardURI | info:eu-repo/grantAgreement/FCT/POR_CENTRO/2021.06044.BD/PT | |
| oaire.citation.startPage | 107520 | |
| oaire.citation.title | Journal of Drug Delivery Science and Technology | |
| oaire.citation.volume | 114 | |
| oaire.fundingStream | CEEC IND4ed | |
| oaire.fundingStream | Concurso de Projetos de I&D em Todos os Domínios Científicos - 2022 | |
| oaire.fundingStream | POR_CENTRO | |
| oaire.version | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |
| person.familyName | Gonçalves | |
| person.familyName | Melo | |
| person.familyName | Pouso | |
| person.familyName | Correia | |
| person.familyName | de Melo-Diogo | |
| person.givenName | Joaquim | |
| person.givenName | Bruna Daniela Lopes | |
| person.givenName | Manuel António do Rosário | |
| person.givenName | Ilídio Joaquim Sobreira | |
| person.givenName | Duarte | |
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| person.identifier.ciencia-id | 5717-CB52-B4E5 | |
| person.identifier.ciencia-id | EB15-3F85-263D | |
| person.identifier.ciencia-id | F610-7373-DC81 | |
| person.identifier.ciencia-id | 9C10-9BD8-634E | |
| person.identifier.orcid | 0009-0008-2314-701X | |
| person.identifier.orcid | 0000-0002-0078-1877 | |
| person.identifier.orcid | 0000-0001-9816-6284 | |
| person.identifier.orcid | 0000-0003-1613-9675 | |
| person.identifier.orcid | 0000-0001-9984-3603 | |
| person.identifier.scopus-author-id | 7003557499 | |
| person.identifier.scopus-author-id | 56266511300 | |
| project.funder.identifier | http://doi.org/10.13039/501100001871 | |
| project.funder.identifier | http://doi.org/10.13039/501100001871 | |
| project.funder.identifier | http://doi.org/10.13039/501100001871 | |
| project.funder.name | Fundação para a Ciência e a Tecnologia | |
| project.funder.name | Fundação para a Ciência e a Tecnologia | |
| project.funder.name | Fundação para a Ciência e a Tecnologia | |
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