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Assembly of breast cancer heterotypic spheroids on hyaluronic acid coated surfaces

2017-05-25Journal article Restricted access
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dc.contributor.authorCarvalho, Marco António Paulo de
dc.contributor.authorCosta, Elisabete C.
dc.contributor.authorCorreia, I.J.
dc.date.accessioned2018-03-22T09:13:48Z
dc.date.available2018-03-22T09:13:48Z
dc.date.issued2017-05-25
dc.description.abstractDrug screening is currently demanding for realistic models that are able to reproduce the structural features of solid tumors. 3D cell culture systems, namely spheroids, emerged as a promising approach to provide reliable results during drug development. So far, liquid overlay technique (LOT) is one of the most used methods for spheroids assembly. It comprises cellular aggregation due to their limited adhesion to certain biomaterials, like agarose. However, researchers are currently improving this technique in order to obtain spheroids on surfaces that mimic cancer extracellular matrix (ECM), since cell–ECM interactions modulate cells behavior and their drug resistance profile. Herein, hyaluronic acid (HA) coated surfaces were used, for the first time, for the production of reproducible heterotypic breast cancer spheroids. The obtained results revealed that it is possible to control the size, shape, and number of spheroids gotten per well by changing the HA concentration and the number of cells initially seeded in each well.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationCarvalho, M. P., Costa, E. C., e Correia, I. J. (2017). “Assembly of breast cancer heterotypic spheroids on Hyaluronic Acid coated surfaces.” Biotechnology Progress, Vol. 33(5), pp.1346-57pt_PT
dc.identifier.doi10.1002/btpr.2497pt_PT
dc.identifier.urihttp://hdl.handle.net/10400.6/4699
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherWileypt_PT
dc.relationHealth Sciences Research Centre
dc.relation3D multicellular spheroids as high throughput platforms to screen novel combinatory therapies for treatment of pancreatic cancer
dc.relation.publisherversionhttps://onlinelibrary.wiley.com/doi/abs/10.1002/btpr.2497pt_PT
dc.subjectBreast tumorpt_PT
dc.subjectHApt_PT
dc.subjectIn vitro modelspt_PT
dc.subjectLOTpt_PT
dc.subjectSpheroidspt_PT
dc.titleAssembly of breast cancer heterotypic spheroids on hyaluronic acid coated surfacespt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleHealth Sciences Research Centre
oaire.awardTitle3D multicellular spheroids as high throughput platforms to screen novel combinatory therapies for treatment of pancreatic cancer
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UID%2FMulti%2F00709%2F2013/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT//SFRH%2FBD%2F103507%2F2014/PT
oaire.citation.endPage1357pt_PT
oaire.citation.startPage1346pt_PT
oaire.citation.titleBiotechnology Progresspt_PT
oaire.citation.volume33pt_PT
oaire.fundingStream6817 - DCRRNI ID
person.familyNameda Rocha Costa
person.familyNameJoaquim Sobreira Correia
person.givenNameElisabete Cristina
person.givenNameIlídio
person.identifierUMbJ1KMAAAAJ
person.identifier.ciencia-idB314-4CF0-6633
person.identifier.ciencia-idF610-7373-DC81
person.identifier.orcid0000-0002-0490-0095
person.identifier.orcid0000-0003-1613-9675
person.identifier.scopus-author-id55805850100
person.identifier.scopus-author-id7003557499
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.embargofctCopyright cedido à editora no momento da publicaçãopt_PT
rcaap.rightsclosedAccesspt_PT
rcaap.typearticlept_PT
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relation.isAuthorOfPublicationf2c3e779-34a2-4309-950a-80687664c6ad
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