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Bacteriostatic versus bactericidal activity of ciprofloxacin in Escherichia coli assessed by flow cytometry using a novel far-red dye

2011-04Journal article Restricted access
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dc.contributor.authorSilva, Filomena
dc.contributor.authorLourenço, Olga
dc.contributor.authorQueiroz, João
dc.contributor.authorDomingues, F.C.
dc.date.accessioned2020-03-02T14:16:56Z
dc.date.available2020-03-02T14:16:56Z
dc.date.issued2011-04
dc.description.abstractAs common microbiological methods for the assessment of bacteriostatic or bactericidal activities are very time-consuming, in this work we describe that the use of a novel far-red fluorescent stain, Vybrant DyeCycle Ruby (DCR) for the flow cytometric analysis of fluoroquinolone (ciprofloxacin) bacteriostatic and bactericidal activities in Escherichia coli proved to be specific for bacterial DNA and, after ciprofloxacin exposure, DNA distribution analysis was achieved using a 7.5 μM DCR concentration to stain 5 × 10⁵ ethanol-fixed bacterial cells. The analysis of the bacterial DNA histograms obtained from the ciprofloxacin concentrations tested, enabled the distinction between ciprofloxacin bacteriostatic and bactericidal activities.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.doi10.1038/ja.2011.5pt_PT
dc.identifier.urihttp://hdl.handle.net/10400.6/9657
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.subjectAnti-Bacterial Agentspt_PT
dc.subjectCiprofloxacinpt_PT
dc.subjectDNA Bacterialpt_PT
dc.subjectDose-Response Relationship Drugpt_PT
dc.subjectEscherichia colipt_PT
dc.subjectFlow Cytometrypt_PT
dc.subjectFluorescent Dyespt_PT
dc.subjectMicrobial Sensitivity Testspt_PT
dc.titleBacteriostatic versus bactericidal activity of ciprofloxacin in Escherichia coli assessed by flow cytometry using a novel far-red dyept_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/3599-PPCDT/PTDC%2FEQU-EQU%2F65492%2F2006/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/SFRH/SFRH%2FBD%2F41521%2F2007/PT
oaire.citation.endPage325pt_PT
oaire.citation.issue4pt_PT
oaire.citation.startPage321-5pt_PT
oaire.citation.titleJournal of Antibioticspt_PT
oaire.citation.volume64pt_PT
oaire.fundingStream3599-PPCDT
oaire.fundingStreamSFRH
person.familyNameSilva
person.familyNameLourenço
person.familyNameQueiroz
person.familyNameDomingues
person.givenNameFilomena
person.givenNameOlga Maria Marques
person.givenNameJoão
person.givenNameFernanda
person.identifier1396147
person.identifier.ciencia-idF91E-2365-F208
person.identifier.ciencia-id6618-1C4C-D1B7
person.identifier.ciencia-id931E-B66D-E341
person.identifier.ciencia-id6514-0498-3E8F
person.identifier.orcid0000-0003-2931-4583
person.identifier.orcid0000-0002-8401-5976
person.identifier.orcid0000-0002-3096-8325
person.identifier.orcid0000-0002-9540-0853
person.identifier.ridA-3091-2013
person.identifier.ridL-3104-2014
person.identifier.ridN-7295-2013
person.identifier.scopus-author-id35723100200
person.identifier.scopus-author-id7003705645
person.identifier.scopus-author-id10139481400
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.embargofctCopyright cedido à editora no momento da publicaçãopt_PT
rcaap.rightsclosedAccesspt_PT
rcaap.typearticlept_PT
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