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Long- and short-term effects of androgens in human umbilical artery smooth muscle

2013-03Journal article Restricted access
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dc.contributor.authorSaldanha, Paulo
dc.contributor.authorCairrão, Elisa
dc.contributor.authorBaptista, Cláudio
dc.contributor.authorVerde, Ignacio
dc.date.accessioned2019-11-28T17:01:41Z
dc.date.available2019-11-28T17:01:41Z
dc.date.issued2013-03
dc.description.abstractThe aim of the present study was to determine the effects of androgens in the regulation of human umbilical artery (HUA) contractility. The short-term effects of testosterone on the tone of the HUA were investigated, as were the long-term effects of dihydrotestosterone (DHT) on the expression of some proteins involved in the contractile process. Endothelium-denuded HUA were treated for 24 h with DHT (2 μmol/L) or the vehicle control (ethanol) to analyse the genomic effects of androgens. Twenty-four hour treatment of HUA with DHT increased the mRNA expression of the β(1)-subunit of the large-conductance Ca(2+)-activated (BK(Ca)) channel and decreased expression of the α-subunit of L-type calcium channels. In organ bath studies, testosterone (1-100 μmol/L) produced similar relaxant responses in DHT- and vehicle-treated HUA rings precontracted with 5-HT, histamine and KCl. However, the relaxation response obtained by the combined application of testosterone (100 μmol/L) and nifedipine (10 μmol/L) was significantly greater in DHT- compared with vehicle-treated HUA. The results indicate that the rapid vasorelaxant effects of testosterone that are dependent on both BK(Ca) and voltage-sensitive potassium (K(V)) channel activity in control arteries become dependent solely on K(V) channel activity in DHT-treated HUA. Thus, the present study reveals the importance of the investigation of both the short- and long-term effects of androgens in human arteries.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.doi10.1111/1440-1681.12047pt_PT
dc.identifier.urihttp://hdl.handle.net/10400.6/7639
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.subjectAndrogenspt_PT
dc.subjectCalcium channelspt_PT
dc.subjectPotassium channelspt_PT
dc.subjectSmooth muscle cellpt_PT
dc.subjectVasorelaxationpt_PT
dc.titleLong- and short-term effects of androgens in human umbilical artery smooth musclept_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage189pt_PT
oaire.citation.issue3pt_PT
oaire.citation.startPage181pt_PT
oaire.citation.titleClinical and Experimental Pharmacology and Physiologypt_PT
oaire.citation.volume40pt_PT
person.familyNameSaldanha
person.familyNameCairrao Rodrigues Oliveira
person.familyNameMaia Baptista
person.familyNameVerde
person.givenNamePaulo
person.givenNameMaria Elisa
person.givenNameCláudio Jorge
person.givenNameIgnacio
person.identifierAAB-1164-2020
person.identifierhttps://scholar.google.pt/citations?user=hFKOd_QAAAAJ&hl=pt-PT&oi=ao
person.identifier.ciencia-id0611-71B8-191E
person.identifier.ciencia-id5115-FCBD-A31F
person.identifier.ciencia-idD012-1D7C-791A
person.identifier.orcid0000-0002-1005-2467
person.identifier.orcid0000-0002-4823-5701
person.identifier.orcid0000-0002-5658-5445
person.identifier.orcid0000-0003-3492-5725
person.identifier.ridJ-2590-2013
person.identifier.ridM-3991-2013
person.identifier.scopus-author-idScopus Author ID: 23476537500
person.identifier.scopus-author-id23097426600
person.identifier.scopus-author-id55913729400
rcaap.embargofctCopyright cedido à editora no momento da publicaçãopt_PT
rcaap.rightsclosedAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublication341a3382-dec7-4889-b2e1-1a29306ed125
relation.isAuthorOfPublication2718c108-c03e-44a3-98a7-b05366e0fe61
relation.isAuthorOfPublication1080c532-c737-4b60-bc44-1d18e3c877d7
relation.isAuthorOfPublicationbfb58284-b817-4267-9628-8fa150aa681c
relation.isAuthorOfPublication.latestForDiscovery341a3382-dec7-4889-b2e1-1a29306ed125

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