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Modification of microfiltration membranes by hydrogel impregnation for pDNA purification

2015-03-02Journal article Restricted access
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dc.contributor.authorCastilho, Pedro Henrique Barata
dc.contributor.authorCorreia, Tiago R.
dc.contributor.authorAmorim, Maria T. Pessoa de
dc.contributor.authorEscobar, Isabel
dc.contributor.authorQueiroz, João
dc.contributor.authorCorreia, Ilídio Joaquim Sobreira
dc.contributor.authorMorão, António
dc.date.accessioned2018-03-20T15:33:51Z
dc.date.available2018-03-20T15:33:51Z
dc.date.issued2015-03-02
dc.description.abstractThe huge efforts for the achievement of highly purified biomolecules are growing every day. A great number of efficient techniques, such as chromatography, are already available in laboratory for separation processes. However, membrane‐based technologies are the best match to assure simplicity, efficiency and easy scale‐up procedures. Herein we report the modification of a commercial microfiltration membrane for plasmid DNA purification by agarose gel impregnation. The membrane was characterized by SEM, ATR‐FTIR, EDS, contact angle, and porosity measurements. Additionally, the membrane pore radius was estimated from observed rejections of different proteins and with that information the rejection of a 6050 bp plasmid DNA (pDNA) molecule was estimated for different values of flux using a theoretical model of large flexible molecules in membranes with parallel cylindrical pores, which is applicable to pDNA ultrafiltration in conventional membranes, as recently shown in the literature. The experimental results show that the modified membrane has higher pDNA rejections than the predicted by the model, suggesting that the different type of porous structure that a hydrogel has, may have a positive effect on pDNA rejections as compared to other biomolecules with more rigid structures, making this type of modified membranes potential better candidates to be used for the selective recovery of pDNA in this type of bioprocesses.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationCastilho, P.H., Correia, T.R., Amorim, M.T.P., Escobar, I.C., Queiroz, J.A., Correia, I.J. e Morão A.M. (2015) “Modification of microfiltration membranes by hydrogel impregnation for pDNA purification", Journal of Applied Polymer Science, Vol. 132(21): pp. 41610pt_PT
dc.identifier.doi10.1002/app.41610pt_PT
dc.identifier.urihttp://hdl.handle.net/10400.6/4665
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherWileypt_PT
dc.relationDESENVOLVIMENTO DE MEMBRANAS DE ULTRAFILTRAÇÃO PARA RECUPERAÇÃO DE DNA PLASMÍDICO A PARTIR DE CALDOS DE FERMENTAÇÃO
dc.relationStrategic Project - UI 709 - 2011-2012
dc.relation2014 - Strategic Project
dc.relation.publisherversionhttps://onlinelibrary.wiley.com/doi/abs/10.1002/app.41610pt_PT
dc.subjectMembranespt_PT
dc.subjectNucleic acidspt_PT
dc.subjectPolysaccharidespt_PT
dc.titleModification of microfiltration membranes by hydrogel impregnation for pDNA purificationpt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleDESENVOLVIMENTO DE MEMBRANAS DE ULTRAFILTRAÇÃO PARA RECUPERAÇÃO DE DNA PLASMÍDICO A PARTIR DE CALDOS DE FERMENTAÇÃO
oaire.awardTitleStrategic Project - UI 709 - 2011-2012
oaire.awardTitle2014 - Strategic Project
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/FARH/SFRH%2FBPD%2F88838%2F2012/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6820 - DCRRNI ID/PEst-C%2FSAU%2FUI0709%2F2011/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/PEst-OE%2FSAU%2FUI0709%2F2014/PT
oaire.citation.startPage41610pt_PT
oaire.citation.titleJournal of Applied Polymer Sciencept_PT
oaire.citation.volume132pt_PT
oaire.fundingStreamFARH
oaire.fundingStream6820 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
person.familyNameCorreia
person.familyNameAmorim
person.familyNameEscobar
person.familyNameQueiroz
person.familyNameJoaquim Sobreira Correia
person.familyNameMorão
person.givenNameTiago
person.givenNameMaria
person.givenNameIsabel
person.givenNameJoão
person.givenNameIlídio
person.givenNameAntónio
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person.identifier.ciencia-idF610-7373-DC81
person.identifier.orcid0000-0002-4077-4698
person.identifier.orcid0000-0003-1516-8407
person.identifier.orcid0000-0001-9269-5927
person.identifier.orcid0000-0002-3096-8325
person.identifier.orcid0000-0003-1613-9675
person.identifier.orcid0000-0001-7173-9716
person.identifier.ridO-4614-2015
person.identifier.ridL-3104-2014
person.identifier.ridI-4887-2013
person.identifier.scopus-author-id55670188900
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project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.embargofctCopyright cedido à editora no momento da publicaçãopt_PT
rcaap.rightsclosedAccesspt_PT
rcaap.typearticlept_PT
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