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Enzymatic removal of plant residues from wool: Application of experimental design techniques for optimization parameters

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dc.contributor.authorGouveia, Isabel C.
dc.contributor.authorFiadeiro, José
dc.contributor.authorQueiroz, João
dc.date.accessioned2022-01-17T10:36:06Z
dc.date.available2022-01-17T10:36:06Z
dc.date.issued2008
dc.description.abstractThis study was undertaken to find the optimum conditions of a new enzymatic process to remove plant residues from wool. Commercial enzymatic preparations of Celluclast 1.5 L and Pectinex Ultra SP-L were selected in order to hydrolyze the polysaccharides in primary plant cell walls and middle lamella, resulting into more fragile residues easier to be removed. Since it was intended to define the optimal conditions for enzyme application, a four-factor central composite design was selected to study the effects of pH, temperature, enzyme concentration and wetting agent concentration, on the two selected responses, i.e., soluble reducing sugars (RS) and alkali solubility (AS) of wool to detect plant degradation and to evaluate wool quality, respectively. Results demonstrated that enzyme concentration was the most significant effect in plant residues degradation. A total enzyme concentration loading of 20 mL of both diluted enzymatic preparations in equal parts per 1 L of incubation solution (42.970 U/L of Celluclast preparation and PG 29.3 nkat/L + PME 2.537 nkat/L of Pectinex preparation), yielded an equivalent amount of 240.127 mg of glucose per 1.0 g of plant residue, at the optimal conditions: 40.56 ◦C, pH 4.0 and 1 mL Plurafac/L. SEM analysis has indicated an identical and important degradation of the plant residues, when compared to the conventional carbonization process, and wool quality has been preserved.pt_PT
dc.description.sponsorshipThe authors would like to thank the R&D Unit of Textile and Paper Materials of the University of Beira Interior for the financial support and the Fitecom industries (Covilha, Portugal) for supply- ˜ ing wool materials and enable to test the optimized process at an industrial scale.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.doi10.1016/j.bej.2008.04.008pt_PT
dc.identifier.urihttp://hdl.handle.net/10400.6/11839
dc.language.isoengpt_PT
dc.subjectBiodegradationpt_PT
dc.subjectCell-wall-polysaccharidespt_PT
dc.subjectEnzyme technologypt_PT
dc.subjectResponse surface methodologypt_PT
dc.subjectModelingpt_PT
dc.subjectOptimizationpt_PT
dc.titleEnzymatic removal of plant residues from wool: Application of experimental design techniques for optimization parameterspt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage165pt_PT
oaire.citation.issue2pt_PT
oaire.citation.startPage157pt_PT
oaire.citation.titleBiochemical Engineering Journalpt_PT
oaire.citation.volume41pt_PT
person.familyNameGouveia
person.familyNameQueiroz
person.givenNameIsabel Cristina
person.givenNameJoão
person.identifier626131
person.identifier.ciencia-id5711-86D0-A54B
person.identifier.ciencia-id931E-B66D-E341
person.identifier.orcid0000-0003-3290-9529
person.identifier.orcid0000-0002-3096-8325
person.identifier.ridK-1651-2014
person.identifier.ridL-3104-2014
person.identifier.scopus-author-id24328741500
person.identifier.scopus-author-id7003705645
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublicationa2024ec6-6edb-47e5-b8a0-594066a5036c
relation.isAuthorOfPublicationc798c033-c160-4f99-a8ad-04416923943f
relation.isAuthorOfPublication.latestForDiscoverya2024ec6-6edb-47e5-b8a0-594066a5036c

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