Publication
Implications of epigallocatechin-3-gallate in cultured human Sertoli cells glycolytic and oxidative profile
dc.contributor.author | Dias, Tânia R. | |
dc.contributor.author | Alves, Marco G | |
dc.contributor.author | Silva, Joaquina | |
dc.contributor.author | Barros, Alberto | |
dc.contributor.author | Sousa, Mário | |
dc.contributor.author | Casal, Susana | |
dc.contributor.author | Silva, Branca M. | |
dc.contributor.author | Oliveira, Pedro F. | |
dc.date.accessioned | 2020-02-17T11:19:40Z | |
dc.date.available | 2020-02-17T11:19:40Z | |
dc.date.issued | 2017 | |
dc.description.abstract | Sertoli cells are crucial for the success of spermatogenesis, which is the biological process that ensures male fertility. These cells present high metabolic rates, being often subjected to high oxidative stress levels that, if uncontrolled, may compromise male fertility. Since the most abundant tea catechin, epigallocatechin-3-gallate (EGCG), has demonstrated a potent preventive activity against oxidative stress, we have evaluated its effect at concentrations of 5 and 50μM, on the metabolism, mitochondrial functionality and oxidative profile of human Sertoli cells (hSCs). While, the highest concentration of EGCG (50μM) increased glucose and pyruvate consumption, it decreased the conversion of pyruvate to alanine to sustain a regular lactate production. However, despite maintaining Krebs cycle functionality, EGCG (50μM) decreased mitochondrial membrane potential of hSCs, which could compromise the normal rates of ATP production. Interestingly, oxidative damages to proteins and lipids decreased in this experimental group, which may be valuable for the nutritional support of spermatogenesis. | pt_PT |
dc.description.version | info:eu-repo/semantics/publishedVersion | pt_PT |
dc.identifier.doi | 10.1016/j.tiv.2017.03.006 | pt_PT |
dc.identifier.uri | http://hdl.handle.net/10400.6/9281 | |
dc.language.iso | eng | pt_PT |
dc.peerreviewed | yes | pt_PT |
dc.relation | White tea WTEA as a promising agent against sufertility/infetility promoted by diabetes mellitus: synesgistic or single component effect | |
dc.relation | Obesitility – Leptin-ghrelin axis and testicular metabolism: mechanisms associated with obesity-related male infertility | |
dc.relation | Obesitility – Gut hormones and testicular metabolism: mechanisms associated with obesity-related male infertility | |
dc.subject | Alanine | pt_PT |
dc.subject | Catechin | pt_PT |
dc.subject | Cell Proliferation | pt_PT |
dc.subject | Cells Cultured | pt_PT |
dc.subject | Glucose | pt_PT |
dc.subject | Glucose Transporter Type 2 | pt_PT |
dc.subject | Glycolysis | pt_PT |
dc.subject | Humans | pt_PT |
dc.subject | L-Lactate Dehydrogenase | pt_PT |
dc.subject | Male | pt_PT |
dc.subject | Membrane Potential Mitochondrial | pt_PT |
dc.subject | Monocarboxylic Acid Transporters | pt_PT |
dc.subject | Muscle Proteins | pt_PT |
dc.subject | Oxidative Stress | pt_PT |
dc.subject | Pyruvic Acid | pt_PT |
dc.subject | Sertoli Cells | pt_PT |
dc.title | Implications of epigallocatechin-3-gallate in cultured human Sertoli cells glycolytic and oxidative profile | pt_PT |
dc.type | journal article | |
dspace.entity.type | Publication | |
oaire.awardTitle | White tea WTEA as a promising agent against sufertility/infetility promoted by diabetes mellitus: synesgistic or single component effect | |
oaire.awardTitle | Obesitility – Leptin-ghrelin axis and testicular metabolism: mechanisms associated with obesity-related male infertility | |
oaire.awardTitle | Obesitility – Gut hormones and testicular metabolism: mechanisms associated with obesity-related male infertility | |
oaire.awardURI | info:eu-repo/grantAgreement/FCT/POR_NORTE/SFRH%2FBD%2F109284%2F2015/PT | |
oaire.awardURI | info:eu-repo/grantAgreement/FCT/SFRH/SFRH%2FBPD%2F80451%2F2011/PT | |
oaire.awardURI | info:eu-repo/grantAgreement/FCT//SFRH%2FBPD%2F108837%2F2015/PT | |
oaire.awardURI | info:eu-repo/grantAgreement/FCT/5876/UID%2FMulti%2F00709%2F2013/PT | |
oaire.awardURI | info:eu-repo/grantAgreement/FCT/5876/PEst-OE%2FSAU%2FUI0215%2F2014/PT | |
oaire.awardURI | info:eu-repo/grantAgreement/FCT/5876/UID%2FQUI%2F50006%2F2013/PT | |
oaire.awardURI | info:eu-repo/grantAgreement/FCT/9471 - RIDTI/PTDC%2FBIM-MET%2F4712%2F2014/PT | |
oaire.awardURI | info:eu-repo/grantAgreement/FCT/9471 - RIDTI/PTDC%2FBBB-BQB%2F1368%2F2014/PT | |
oaire.citation.endPage | 222 | pt_PT |
oaire.citation.startPage | 214 | pt_PT |
oaire.citation.title | Toxicology in Vitro | pt_PT |
oaire.citation.volume | 41 | pt_PT |
oaire.fundingStream | POR_NORTE | |
oaire.fundingStream | SFRH | |
oaire.fundingStream | 5876 | |
oaire.fundingStream | 5876 | |
oaire.fundingStream | 5876 | |
oaire.fundingStream | 9471 - RIDTI | |
oaire.fundingStream | 9471 - RIDTI | |
person.familyName | Dias | |
person.familyName | Alves | |
person.familyName | Casal | |
person.familyName | Silva | |
person.familyName | Oliveira | |
person.givenName | Tânia | |
person.givenName | Marco G. | |
person.givenName | Susana | |
person.givenName | Branca Maria | |
person.givenName | Pedro Fontes | |
person.identifier | 1033309 | |
person.identifier | 2358493 | |
person.identifier.ciencia-id | C11D-CE16-7D36 | |
person.identifier.ciencia-id | 7915-53C7-40C2 | |
person.identifier.ciencia-id | F915-3FEA-C748 | |
person.identifier.ciencia-id | BD1F-B24D-89A9 | |
person.identifier.ciencia-id | 9F16-B704-C407 | |
person.identifier.orcid | 0000-0002-2336-8006 | |
person.identifier.orcid | 0000-0001-7635-783X | |
person.identifier.orcid | 0000-0002-1686-3850 | |
person.identifier.orcid | 0000-0002-5610-8024 | |
person.identifier.orcid | 0000-0002-4989-5699 | |
person.identifier.rid | G-4795-2010 | |
person.identifier.scopus-author-id | 55619567500 | |
person.identifier.scopus-author-id | 57202054274 | |
person.identifier.scopus-author-id | 7005615589 | |
person.identifier.scopus-author-id | 36198848500 | |
project.funder.identifier | http://doi.org/10.13039/501100001871 | |
project.funder.identifier | http://doi.org/10.13039/501100001871 | |
project.funder.identifier | http://doi.org/10.13039/501100001871 | |
project.funder.identifier | http://doi.org/10.13039/501100001871 | |
project.funder.identifier | http://doi.org/10.13039/501100001871 | |
project.funder.identifier | http://doi.org/10.13039/501100001871 | |
project.funder.identifier | http://doi.org/10.13039/501100001871 | |
project.funder.identifier | http://doi.org/10.13039/501100001871 | |
project.funder.name | Fundação para a Ciência e a Tecnologia | |
project.funder.name | Fundação para a Ciência e a Tecnologia | |
project.funder.name | Fundação para a Ciência e a Tecnologia | |
project.funder.name | Fundação para a Ciência e a Tecnologia | |
project.funder.name | Fundação para a Ciência e a Tecnologia | |
project.funder.name | Fundação para a Ciência e a Tecnologia | |
project.funder.name | Fundação para a Ciência e a Tecnologia | |
project.funder.name | Fundação para a Ciência e a Tecnologia | |
rcaap.rights | openAccess | pt_PT |
rcaap.type | article | pt_PT |
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