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Implications of epigallocatechin-3-gallate in cultured human Sertoli cells glycolytic and oxidative profile

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dc.contributor.authorDias, Tânia R.
dc.contributor.authorAlves, Marco G
dc.contributor.authorSilva, Joaquina
dc.contributor.authorBarros, Alberto
dc.contributor.authorSousa, Mário
dc.contributor.authorCasal, Susana
dc.contributor.authorSilva, Branca M.
dc.contributor.authorOliveira, Pedro F.
dc.date.accessioned2020-02-17T11:19:40Z
dc.date.available2020-02-17T11:19:40Z
dc.date.issued2017
dc.description.abstractSertoli cells are crucial for the success of spermatogenesis, which is the biological process that ensures male fertility. These cells present high metabolic rates, being often subjected to high oxidative stress levels that, if uncontrolled, may compromise male fertility. Since the most abundant tea catechin, epigallocatechin-3-gallate (EGCG), has demonstrated a potent preventive activity against oxidative stress, we have evaluated its effect at concentrations of 5 and 50μM, on the metabolism, mitochondrial functionality and oxidative profile of human Sertoli cells (hSCs). While, the highest concentration of EGCG (50μM) increased glucose and pyruvate consumption, it decreased the conversion of pyruvate to alanine to sustain a regular lactate production. However, despite maintaining Krebs cycle functionality, EGCG (50μM) decreased mitochondrial membrane potential of hSCs, which could compromise the normal rates of ATP production. Interestingly, oxidative damages to proteins and lipids decreased in this experimental group, which may be valuable for the nutritional support of spermatogenesis.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.doi10.1016/j.tiv.2017.03.006pt_PT
dc.identifier.urihttp://hdl.handle.net/10400.6/9281
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.relationBolsa de Doutoramento FCT: White tea WTEA as a promising agent against sufertility/infetility promoted by diabetes mellitus: synesgistic or single component effect [SFRH/BD/109284/2015]
dc.relationBolsa de pós-doutoramento FCT: Biomedicina e reprodução masculina [SFRH/BPD/80451/2011]
dc.relationBolsa de Pós-Doutoramento FCT: Obesitility – Leptin-ghrelin axis and testicular metabolism: mechanisms associated with obesity-related male infertility [SFRH/BPD/108837/2015]
dc.relationFinanciamento base atribuído ao CICS-UBI [UID/Multi/00709/2013]
dc.relationUMIB - Projeto Estratégico - UI 215 - 2014 [PEst-OE/SAU/UI0215/2014]
dc.relationLaboratório Associado para a Química Verde - Tecnologias e Processos Limpos [UID/QUI/50006/2013]
dc.relationObesitility – Gut hormones and testicular metabolism: mechanisms associated with obesity-related male infertility
dc.relationAQUAPORINAS - Aquaporinas como parceiros moleculares do CFTR na Barreira Hematotesticular: uma intersecção entre a infertilidade masculina humana e a fibrose cística? [PTDC/BBB-BQB/1368/2014]
dc.subjectAlaninept_PT
dc.subjectCatechinpt_PT
dc.subjectCell Proliferationpt_PT
dc.subjectCells Culturedpt_PT
dc.subjectGlucosept_PT
dc.subjectGlucose Transporter Type 2pt_PT
dc.subjectGlycolysispt_PT
dc.subjectHumanspt_PT
dc.subjectL-Lactate Dehydrogenasept_PT
dc.subjectMalept_PT
dc.subjectMembrane Potential Mitochondrialpt_PT
dc.subjectMonocarboxylic Acid Transporterspt_PT
dc.subjectMuscle Proteinspt_PT
dc.subjectOxidative Stresspt_PT
dc.subjectPyruvic Acidpt_PT
dc.subjectSertoli Cellspt_PT
dc.titleImplications of epigallocatechin-3-gallate in cultured human Sertoli cells glycolytic and oxidative profilept_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardNumberSFRH/BD/109284/2015
oaire.awardNumberSFRH/BPD/80451/2011
oaire.awardNumberSFRH/BPD/108837/2015
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oaire.awardTitleBolsa de Doutoramento FCT: White tea WTEA as a promising agent against sufertility/infetility promoted by diabetes mellitus: synesgistic or single component effect [SFRH/BD/109284/2015]
oaire.awardTitleBolsa de pós-doutoramento FCT: Biomedicina e reprodução masculina [SFRH/BPD/80451/2011]
oaire.awardTitleBolsa de Pós-Doutoramento FCT: Obesitility – Leptin-ghrelin axis and testicular metabolism: mechanisms associated with obesity-related male infertility [SFRH/BPD/108837/2015]
oaire.awardTitleFinanciamento base atribuído ao CICS-UBI [UID/Multi/00709/2013]
oaire.awardTitleUMIB - Projeto Estratégico - UI 215 - 2014 [PEst-OE/SAU/UI0215/2014]
oaire.awardTitleLaboratório Associado para a Química Verde - Tecnologias e Processos Limpos [UID/QUI/50006/2013]
oaire.awardTitleObesitility – Gut hormones and testicular metabolism: mechanisms associated with obesity-related male infertility
oaire.awardTitleAQUAPORINAS - Aquaporinas como parceiros moleculares do CFTR na Barreira Hematotesticular: uma intersecção entre a infertilidade masculina humana e a fibrose cística? [PTDC/BBB-BQB/1368/2014]
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oaire.citation.endPage222pt_PT
oaire.citation.startPage214pt_PT
oaire.citation.titleToxicology in Vitropt_PT
oaire.citation.volume41pt_PT
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person.givenNameTânia
person.givenNameMarco G.
person.givenNameSusana
person.givenNameBranca Maria
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rcaap.typearticlept_PT
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