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Implications of epigallocatechin-3-gallate in cultured human Sertoli cells glycolytic and oxidative profile

2017Journal article Open access
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dc.contributor.authorDias, Tânia R.
dc.contributor.authorAlves, Marco G
dc.contributor.authorSilva, Joaquina
dc.contributor.authorBarros, Alberto
dc.contributor.authorSousa, Mário
dc.contributor.authorCasal, Susana
dc.contributor.authorSilva, Branca M.
dc.contributor.authorOliveira, Pedro F.
dc.date.accessioned2020-02-17T11:19:40Z
dc.date.available2020-02-17T11:19:40Z
dc.date.issued2017
dc.description.abstractSertoli cells are crucial for the success of spermatogenesis, which is the biological process that ensures male fertility. These cells present high metabolic rates, being often subjected to high oxidative stress levels that, if uncontrolled, may compromise male fertility. Since the most abundant tea catechin, epigallocatechin-3-gallate (EGCG), has demonstrated a potent preventive activity against oxidative stress, we have evaluated its effect at concentrations of 5 and 50μM, on the metabolism, mitochondrial functionality and oxidative profile of human Sertoli cells (hSCs). While, the highest concentration of EGCG (50μM) increased glucose and pyruvate consumption, it decreased the conversion of pyruvate to alanine to sustain a regular lactate production. However, despite maintaining Krebs cycle functionality, EGCG (50μM) decreased mitochondrial membrane potential of hSCs, which could compromise the normal rates of ATP production. Interestingly, oxidative damages to proteins and lipids decreased in this experimental group, which may be valuable for the nutritional support of spermatogenesis.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.doi10.1016/j.tiv.2017.03.006pt_PT
dc.identifier.urihttp://hdl.handle.net/10400.6/9281
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.relationWhite tea WTEA as a promising agent against sufertility/infetility promoted by diabetes mellitus: synesgistic or single component effect
dc.relationObesitility – Leptin-ghrelin axis and testicular metabolism: mechanisms associated with obesity-related male infertility
dc.relationObesitility – Gut hormones and testicular metabolism: mechanisms associated with obesity-related male infertility
dc.subjectAlaninept_PT
dc.subjectCatechinpt_PT
dc.subjectCell Proliferationpt_PT
dc.subjectCells Culturedpt_PT
dc.subjectGlucosept_PT
dc.subjectGlucose Transporter Type 2pt_PT
dc.subjectGlycolysispt_PT
dc.subjectHumanspt_PT
dc.subjectL-Lactate Dehydrogenasept_PT
dc.subjectMalept_PT
dc.subjectMembrane Potential Mitochondrialpt_PT
dc.subjectMonocarboxylic Acid Transporterspt_PT
dc.subjectMuscle Proteinspt_PT
dc.subjectOxidative Stresspt_PT
dc.subjectPyruvic Acidpt_PT
dc.subjectSertoli Cellspt_PT
dc.titleImplications of epigallocatechin-3-gallate in cultured human Sertoli cells glycolytic and oxidative profilept_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleWhite tea WTEA as a promising agent against sufertility/infetility promoted by diabetes mellitus: synesgistic or single component effect
oaire.awardTitleObesitility – Leptin-ghrelin axis and testicular metabolism: mechanisms associated with obesity-related male infertility
oaire.awardTitleObesitility – Gut hormones and testicular metabolism: mechanisms associated with obesity-related male infertility
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/POR_NORTE/SFRH%2FBD%2F109284%2F2015/PT
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oaire.citation.endPage222pt_PT
oaire.citation.startPage214pt_PT
oaire.citation.titleToxicology in Vitropt_PT
oaire.citation.volume41pt_PT
oaire.fundingStreamPOR_NORTE
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oaire.fundingStream9471 - RIDTI
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person.familyNameDias
person.familyNameAlves
person.familyNameCasal
person.familyNameSilva
person.familyNameOliveira
person.givenNameTânia
person.givenNameMarco G.
person.givenNameSusana
person.givenNameBranca Maria
person.givenNamePedro Fontes
person.identifier1033309
person.identifier2358493
person.identifier.ciencia-idC11D-CE16-7D36
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person.identifier.ciencia-idBD1F-B24D-89A9
person.identifier.ciencia-id9F16-B704-C407
person.identifier.orcid0000-0002-2336-8006
person.identifier.orcid0000-0001-7635-783X
person.identifier.orcid0000-0002-1686-3850
person.identifier.orcid0000-0002-5610-8024
person.identifier.orcid0000-0002-4989-5699
person.identifier.ridG-4795-2010
person.identifier.scopus-author-id55619567500
person.identifier.scopus-author-id57202054274
person.identifier.scopus-author-id7005615589
person.identifier.scopus-author-id36198848500
project.funder.identifierhttp://doi.org/10.13039/501100001871
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project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
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project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
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