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Effect of amniotic membrane proteins in human cancer cell lines: an exploratory study

2014-04Journal article Restricted access
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dc.contributor.authorMamede, Ana Catarina Manjolinha
dc.contributor.authorLaranjo, Marta
dc.contributor.authorCarvalho, Maria J
dc.contributor.authorAbrantes, Ana M
dc.contributor.authorPires, A S
dc.contributor.authorBrito, A F
dc.contributor.authorMoura, P
dc.contributor.authorMaia, C J
dc.contributor.authorBotelho, M F
dc.date.accessioned2019-12-02T16:45:37Z
dc.date.available2019-12-02T16:45:37Z
dc.date.issued2014-04
dc.description.abstractHuman amniotic membrane (hAM) has recently drawn attention as an upcoming anti-cancer therapy. Regarding the strategies which have already investigated, little is known about hAM protein extracts (hAMPE) effect on cancer. So, this work aims to study the effect of hAMPE in metabolic activity of several human cancer cell lines. hAMPE were mechanically obtained, thus avoiding the effect of detergents and other reagents commonly used in protein extraction under the cell lines studied. After quantification of proteins in hAMPE, their effect on the metabolic activity of 21 human cancer cell lines was assessed by 3-(4,5-dimethylthia-zolyl-2)2,5-diphenyltetrazolium bromide (MTT) assay. Our results indicate that there is an inhibition of metabolic activity until 25 and 50% in two and seven cell lines, respectively. Five cell lines proved to be very sensitive to hAMPE, being its metabolic activity more than 50% inhibited. Our results show that hAMPE can inhibit the metabolic activity of some human cancer cell lines. However, research about this cell line-dependent response to hAMPE becomes indispensable.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.doi10.1007/s00232-014-9642-3pt_PT
dc.identifier.urihttp://hdl.handle.net/10400.6/7647
dc.language.isoengpt_PT
dc.relationVITAMINA C E CANCRO. ESTUDO EXPERIMENTAL
dc.subjectAmniotic membranept_PT
dc.subjectCancer Cell Linespt_PT
dc.subjectMetabolic activitypt_PT
dc.subjectMTT Assaypt_PT
dc.titleEffect of amniotic membrane proteins in human cancer cell lines: an exploratory studypt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleVITAMINA C E CANCRO. ESTUDO EXPERIMENTAL
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/SFRH/SFRH%2FBD%2F73649%2F2010/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/SFRH/SFRH%2FBD%2F44957%2F2008/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/SFRH/SFRH%2FSINTD%2F60068%2F2009/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT//SFRH%2FBD%2F75300%2F2010/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/SFRH/SFRH%2FBD%2F61378%2F2009/PT
oaire.citation.endPage360pt_PT
oaire.citation.issue4pt_PT
oaire.citation.startPage357pt_PT
oaire.citation.titleJournal of Membrane Biologypt_PT
oaire.citation.volume247pt_PT
oaire.fundingStreamSFRH
oaire.fundingStreamSFRH
oaire.fundingStreamSFRH
oaire.fundingStreamSFRH
person.familyNameLaranjo
person.familyNameCarvalho
person.familyNameAbrantes
person.familyNamePires Lourenço
person.familyNameMaia Baptista
person.familyNameBotelho
person.givenNameMarta
person.givenNameMaria J
person.givenNameAna M
person.givenNameAna Salomé
person.givenNameCláudio Jorge
person.givenNameMaria Filomena
person.identifierI-6040-2012
person.identifier2085222
person.identifierR-000-10V
person.identifierH-6010-2018
person.identifierhttps://scholar.google.pt/citations?user=hFKOd_QAAAAJ&hl=pt-PT&oi=ao
person.identifierR-000-BFD
person.identifier.ciencia-id861A-EEAC-70E0
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person.identifier.ciencia-idD716-3387-EAC1
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person.identifier.orcid0000-0001-7994-4650
person.identifier.orcid0000-0002-5658-5445
person.identifier.orcid0000-0001-7202-1650
person.identifier.ridG-6913-2018
person.identifier.ridG-1860-2011
person.identifier.scopus-author-id7801545833
person.identifier.scopus-author-id55440132400
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person.identifier.scopus-author-id56401575000
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person.identifier.scopus-author-id7005751167
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
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project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.embargofctCopyright cedido à editora no momento da publicação.pt_PT
rcaap.rightsclosedAccesspt_PT
rcaap.typearticlept_PT
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