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Estrogenic regulation of testicular expression of stem cell factor and c-kit: implications in germ cell survival and male fertility

2014Journal article Open access
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dc.contributor.authorCorreia, Sara
dc.contributor.authorAlves, Mário Rui Castanheira
dc.contributor.authorCavaco, José E.
dc.contributor.authorOliveira, P.F.
dc.contributor.authorSocorro, Sílvia
dc.date.accessioned2025-02-19T17:34:52Z
dc.date.available2025-02-19T17:34:52Z
dc.date.issued2014
dc.description.abstractObjective: To study the effect of estrogens regulating the testicular expression of stem cell factor (SCF) and c-kit. Design: Experimental study. Setting: University research center. Animal(s): Male Wistar rats. Intervention(s): Rat seminiferous tubules (SeT) cultured in the presence or absence of 17β-estradiol (E2). Main outcome measure(s): Expression of SCF and c-kit as well as apoptotic factors, FasL, FasR, Bcl-2, and Bax analyzed via quantitative reverse transcription-polymerase and Western blot; enzymatic activity of apoptosis effector caspase-3 assessed by colorimetric assay; proliferation index in SeT epithelium determined via fluorescent immunohistochemistry of nuclear proliferation marker Ki67. Result(s): E2 (100 nM) induced a decrease in c-kit expression while increasing expression of SCF. Altered expression of the SCF/c-kit system relied on apoptosis of germ cells, as evidenced by the up-regulated expression of FasL/FasR, the increased ratio of proapoptotic/antiapoptotic proteins (Bax/Bcl-2), and the augmented activity of caspase-3. Decreased proliferation was also found in SeT in response to E2. Conclusion(s): A 100 nM dose of E2 unbalance the SCF/c-kit system, with a crucial impact on germ cell survival and thus male fertility. These findings contribute to our knowledge of the mechanisms underlying male idiopathic infertility associated with hyperestrogenism and open new perspectives on treatment targeting estrogen-signaling mechanisms.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.doi10.1016/j.fertnstert.2014.04.009pt_PT
dc.identifier.urihttp://hdl.handle.net/10400.6/15108
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.subjectEstrogenpt_PT
dc.subjectGerm cellpt_PT
dc.subjectApoptosispt_PT
dc.titleEstrogenic regulation of testicular expression of stem cell factor and c-kit: implications in germ cell survival and male fertilitypt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage306pt_PT
oaire.citation.startPage299pt_PT
oaire.citation.titleFertility and Sterilitypt_PT
oaire.citation.volume102pt_PT
person.familyNameCorreia
person.familyNameCavaco
person.familyNameOliveira
person.familyNameSocorro
person.givenNameSara
person.givenNameJose Eduardo
person.givenNamePedro Fontes
person.givenNameSílvia Cristina da Cruz Marques
person.identifier2358493
person.identifierhttps://scholar.google.pt/citations?user=PA3L_h8AAAAJ&hl=pt-PT
person.identifier.ciencia-id7518-7FBC-A42F
person.identifier.ciencia-id9F16-B704-C407
person.identifier.ciencia-idAB17-5C25-1F2C
person.identifier.orcid0000-0001-9199-8360
person.identifier.orcid0000-0002-3879-733X
person.identifier.orcid0000-0002-4989-5699
person.identifier.orcid0000-0001-8181-488X
person.identifier.ridN-7461-2013
person.identifier.ridG-4795-2010
person.identifier.scopus-author-id7004157751
person.identifier.scopus-author-id6603341330
person.identifier.scopus-author-id36198848500
person.identifier.scopus-author-id23097994600
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
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relation.isAuthorOfPublicationaf92f693-46e2-4ecb-a719-526b41ab6735
relation.isAuthorOfPublication1fb06949-0e6c-41a6-bb25-11885e7f0e9a
relation.isAuthorOfPublication.latestForDiscovery049d1335-73d4-46c8-a99d-b96d9e996f17

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