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Isolation of Human Umbilical Arterial Smooth Muscle Cells (HUASMC)

2010-03-07Journal article Restricted access
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dc.contributor.authorRibeiro, MP.
dc.contributor.authorRelvas, Ricardo
dc.contributor.authorChiquita, Samuel
dc.contributor.authorCorreia, Ilídio Joaquim Sobreira
dc.date.accessioned2018-04-03T16:11:18Z
dc.date.available2018-04-03T16:11:18Z
dc.date.issued2010-03-07
dc.description.abstractThe human umbilical cord (UC) is a biological sample that can be easily obtained just after birth. This biological sample is, most of the time, discarded and their collection does not imply any added risk to the newborn or mother s health. Moreover no ethical concerns are raised. The UC is composed by one vein and two arteries from which both endothelial cells (ECs) 1 and smooth muscle cells (SMCs) 2, two of the main cellular components of blood vessels, can be isolated. In this project the SMCs were obtained after enzymatic treatment of the UC arteries accordingly the experimental procedure previously described by Jaffe et al 3. After cell isolation they were kept in t-flash with DMEM-F12 supplemented with 5% of fetal bovine serum and were cultured for several passages. Cells maintained their morphological and other phenotypic characteristics in the different generations. The aim of this study was to isolate smooth muscle cells in order to use them as models for future assays with constrictor drugs, isolate and structurally characterize L-type calcium channels, to study cellular and molecular aspects of the vascular function 4 and to use them in tissue engineering.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationRibeiro, M.P., Relvas, R., Chiquita, S. e Correia I.J. (2010) “Isolation of Human Umbilical Arterial Smooth Muscle Cells (HUASMC)”, Journal of Visualized Experiments, 41, pii: e1940pt_PT
dc.identifier.doi10.3791/1940pt_PT
dc.identifier.urihttp://hdl.handle.net/10400.6/4733
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherJournal of Visualized Experimentspt_PT
dc.relation.publisherversionhttps://www.jove.com/video/1940/isolation-of-human-umbilical-arterial-smooth-muscle-cells-huasmcpt_PT
dc.subjectUmbilical arterial smooth muscle cellspt_PT
dc.subjectIsolationpt_PT
dc.subjectHuman umbilical cordpt_PT
dc.titleIsolation of Human Umbilical Arterial Smooth Muscle Cells (HUASMC)pt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.startPagee1940pt_PT
oaire.citation.titleJournal of Visualized Experimentspt_PT
oaire.citation.volume41pt_PT
person.familyNameRibeiro
person.familyNameJoaquim Sobreira Correia
person.givenNameMaximiano
person.givenNameIlídio
person.identifierUMbJ1KMAAAAJ
person.identifier.ciencia-idBD11-6497-FD37
person.identifier.ciencia-idF610-7373-DC81
person.identifier.orcid0000-0001-6357-8115
person.identifier.orcid0000-0003-1613-9675
person.identifier.scopus-author-id26658759200
person.identifier.scopus-author-id7003557499
rcaap.embargofctCopyright cedido à editora no momento da publicaçãopt_PT
rcaap.rightsclosedAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublication3fe7a59f-e867-4bed-bb6e-7d7631330af5
relation.isAuthorOfPublicationf2c3e779-34a2-4309-950a-80687664c6ad
relation.isAuthorOfPublication.latestForDiscoveryf2c3e779-34a2-4309-950a-80687664c6ad

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