Departamento de Química
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Browsing Departamento de Química by Author "Afonso, Ângela Maria Cerqueira Coelho"
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- Identification of biomarkers for quality control in human biological serum samplesPublication . Afonso, Ângela Maria Cerqueira Coelho; Lopes, Joana Caetano; Tomaz, Cândida Ascensão TeixeiraEasy access to human biological samples and respective detailed clinical information is pivotal to the progress of medical knowledge, contributing to minimize the direct and indirect health costs and improve the quality of life. The concept of biobank was born in this context, as an infrastructure composed by collections of biological samples and respective clinical information. The impact of biobanks is measured by the presence of good quality samples associated with clinical information. Biobanco-IMM, so far, has more than 80000 samples from more than 10000 donors being the most frequent sample type the serum. Quality control of serum sample has been the subject of intense debate in European meetings of biobanks. Therefore, this study aims to find potential biomarkers for quality control of the serum samples stored at Biobanco-IMM. 200 serum samples were grouped according to the time after sampling, time to freezing, duration of storage or number of freeze-thaw cycles and were compared with a control group of samples that were processed according to the Biobanco-IMM standards. CD40L, GM-CSF, IL- 1a, G-CSF and VEGF were measured in the serum by ELISA. The results show that IL-1a and G-CSF have significant differences in the study groups wherein the analysis of the ROC curves found a cutoff of 33.9 pg/ml with 100% sensitivity for IL-1a, and a cutoff of 78.4 pg/mL with 65% specificity to G-CSF. Thus, these can act as biomarkers for serum quality control.