Logo do repositório

Percorrer por autor "Pires, A S"

A mostrar 1 - 2 de 2
  • Beyond the limits of oxygen: effects of hypoxia in a hormone-independent prostate cancer cell line
    Publication . Mamede, AC; Abrantes, Ana M; Pedrosa, L; Lopes, João Casalta; Pires, A S; Teixo, R J; Gonçalves, A C; Ribeiro, A B Sarmento; Maia, C J; Botelho, M F
    Prostate cancer (PCa) has a high incidence worldwide. One of the major causes of PCa resistance is intratumoral hypoxia. In solid tumors, hypoxia is strongly associated with malignant progression and resistance to therapy, which is an indicator of poor prognosis. The antiproliferative effect and induced death caused by doxorubicin, epirubicin, cisplatin, and flutamide in a hormone-independent PCa cell line will be evaluated. The hypoxia effect on drug resistance to these drugs, as well as cell proliferation and migration, will be also analyzed. All drugs induced an antiproliferative effect and also cell death in the cell line under study. Hypoxia made the cells more resistant to all drugs. Moreover, our results reveal that long time cell exposure to hypoxia decreases cellular proliferation and migration. Hypoxia can influence cellular resistance, proliferation, and migration. This study shows that hypoxia may be a key factor in the regulation of PCa.
    2013Artigo científico Acesso aberto Ver mais
  • Effect of amniotic membrane proteins in human cancer cell lines: an exploratory study
    Publication . Mamede, AC; Laranjo, Marta; Carvalho, Maria J; Abrantes, Ana M; Pires, A S; Brito, A F; Moura, P; Maia, C J; Botelho, M F
    Human amniotic membrane (hAM) has recently drawn attention as an upcoming anti-cancer therapy. Regarding the strategies which have already investigated, little is known about hAM protein extracts (hAMPE) effect on cancer. So, this work aims to study the effect of hAMPE in metabolic activity of several human cancer cell lines. hAMPE were mechanically obtained, thus avoiding the effect of detergents and other reagents commonly used in protein extraction under the cell lines studied. After quantification of proteins in hAMPE, their effect on the metabolic activity of 21 human cancer cell lines was assessed by 3-(4,5-dimethylthia-zolyl-2)2,5-diphenyltetrazolium bromide (MTT) assay. Our results indicate that there is an inhibition of metabolic activity until 25 and 50% in two and seven cell lines, respectively. Five cell lines proved to be very sensitive to hAMPE, being its metabolic activity more than 50% inhibited. Our results show that hAMPE can inhibit the metabolic activity of some human cancer cell lines. However, research about this cell line-dependent response to hAMPE becomes indispensable.
    2014-04Artigo científico Acesso restrito Ver mais