Percorrer por autor "Rocha, Pedro Gabriel Plácido de Mendonça"
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- Tragopogon porrifolius: phytochemical characterization and evaluation of the potential antitumor effect of the extract on the prostate cellsPublication . Rocha, Pedro Gabriel Plácido de Mendonça; Duarte, Ana Paula Coelho; Socorro, Silvia Cristina; Fonseca, Lara Raquel dos SantosThe present document is divided into 2 chapters being part of the unit "Internship in Pharmaceutical Sciences" incorporated in the curricular plan of the Integrated Master´s in Pharmaceutical Sciences, culminating in its defense in public evidence. The first chapter refers to a detailed subjective testimony based of the internship made in Farmácia Modelar from october 2021 to january 2022, under the supervision of technical director João Paiva. During this time, I explored a variety of areas, from management to filling patients prescriptions, gaining practical experience. In the second part, an experimental study was conducted to assess the potential antitumor effect of an extract on prostate cells, under the supervision of teachers Ana Duarte and Silvia Socorro and also master Lara Fonseca. In the previous mentioned study, Tragopogon porrifolius, commonly named salsify, is an edible herb used in traditional medicine with proven anti-tumorigenic activity in human breast and colon cancer cells, controlling oxidative stress, proliferation, and apoptosis. However, the effect of this plant in prostate cancer remains largely unknown. This study aims to investigate the effect of T. porrifolius extract in modulating cell proliferation in human prostate cells. T. porrifolius extract was obtained by an ultrasound-assisted solid-liquid extraction with ethanol/water (70/30) followed by a solvent evaporation in a rotovapor. The chemical characterization was performed by determination of the total phenolic compounds using the Folin-Ciocalteu colorimetric method and the total flavonoid content by the Aluminum Chloride colorimetric method. The antioxidant activity was determined by DPPH and ß-carotene methods. Non-neoplastic (PNT1A) and neoplastic (PC3) cells were treated with 10 – 2000 µg/mL of extract for 24, 48 and 72 hours. The results showed that this plant extract takes time to exercise his effects. Cell viability and proliferation were evaluated by the MTT essay. The T. porrifolius extract exhibited significant contents of total phenolics and total flavonoids, as well as a strong antioxidant activity using the DPPH method in reducing the radical to hydrazine. Although preliminary, the results obtained indicate that T. porrifolius can inhibit prostate cancer cell growth with more pronounced effects in neoplasic cells. These findings support the further investigation on the possible use of this good costaccessibility plant as a new potential therapeutic tool.