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- L-Theanine promotes cultured human Sertoli cells proliferation and modulates glucose metabolismPublication . Dias, T. R.; Bernardino, Raquel; Alves, Marco G; Silva, Joaquina; Barros, Alberto; Sousa, Mário; Casal, Susana; Silva, Branca M.; Oliveira, P.F.L-Theanine is the major free amino acid present in tea (Camellia sinensis L.). The effects of several tea constituents on male reproduction have been investigated, but L-theanine has been overlooked. Sertoli cells (SCs) are essential for the physical and nutritional support of germ cells. In this study, we aimed to investigate the ability of L-theanine to modulate important mechanisms of human SCs (hSCs) metabolism, mitochondrial function and oxidative profile, which are essential to prevent or counteract spermatogenesis disruption in several health conditions.