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Optimization of gold core-mesoporous silica shell functionalization with TPGS and PEI for cancer therapy
Publication . Rodrigues, Ana Carolina Félix; Reis, Catarina A.; Moreira, André; Ferreira, Paula; Correia, I.J.
Photothermal therapy (PTT) has captured the attention of different researchers around the world, since the
application of NIR light responsive-nanomaterials has shown promising results in cancer therapy. Gold-core
mesoporous silica shell (Au-MSS) nanoparticles allow the combination of gold mediated PTT with the drug
delivery in order to improve their therapeutic potential. In this study, two different methodologies, electrostatic
or chemical linkage, were explored to functionalize Au-MSS nanorods with TPGS and PEI. For that purpose, the
TPGS and PEI were chemically coupled to each other or modified with 3-(triethoxysilyl)propyl isocyanate. The
produced Au-MSS nanorods display a uniform morphology and a well-defined gold nucleus and silica shell.
Further, the particles surface charge was dependent on the synthesis methodology. The particles modified by
electrostatic interactions (Au-MSS/TPGS-PEI) were slightly negative (−16.9 and −5.1 mV) whereas the formulations
produced by chemical linkage (Au-MSS/TPGS/PEI) resulted in positively charged nanoparticles (30.9
and 6.8 mV). The successful incorporation of the polymers was confirmed by Fourier Transformed Infrared
spectroscopy and thermogravimetric analysis. Moreover, the Au-MSS functionalization did not affect the particles
PTT capacity. However, the Au-MSS/TPGS/PEI nanorods displayed a decreased drug encapsulation efficiency.
In vitro assays demonstrated the cytocompatibility of Au-MSS up to concentrations of 200 μg/mL,
however the positively charged formulations only remained biocompatible until 100 and 125 μg/mL. Overall,
the attained data confirm the successful modification of Au-MSS nanorods with TPGS and PEI as well as their
applicability as PTT and drug delivery agents.
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Fundação para a Ciência e a Tecnologia
Programa de financiamento
5876
Número da atribuição
UID/Multi/04378/2013