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Optimization of liquid overlay technique to formulate heterogenic 3D co‐cultures models

2014-02-25Journal article Restricted access
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dc.contributor.authorCosta, Elisabete C.
dc.contributor.authorGaspar, Vítor Manuel Abreu
dc.contributor.authorCoutinho, Paula
dc.contributor.authorCorreia, Ilídio Joaquim Sobreira
dc.date.accessioned2018-03-20T10:38:37Z
dc.date.available2018-03-20T10:38:37Z
dc.date.issued2014-02-25
dc.description.abstractThree‐dimensional (3D) cell culture models of solid tumors are currently having a tremendous impact in the in vitro screening of candidate anti‐tumoral therapies. These 3D models provide more reliable results than those provided by standard 2D in vitro cell cultures. However, 3D manufacturing techniques need to be further optimized in order to increase the robustness of these models and provide data that can be properly correlated with the in vivo situation. Therefore, in the present study the parameters used for producing multicellular tumor spheroids (MCTS) by liquid overlay technique (LOT) were optimized in order to produce heterogeneous cellular agglomerates comprised of cancer cells and stromal cells, during long periods. Spheroids were produced under highly controlled conditions, namely: (i) agarose coatings; (ii) horizontal stirring, and (iii) a known initial cell number. The simultaneous optimization of these parameters promoted the assembly of 3D characteristic cellular organization similar to that found in the in vivo solid tumors. Such improvements in the LOT technique promoted the assembly of highly reproducible, individual 3D spheroids, with a low cost of production and that can be used for future in vitro drug screening assays.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationCosta, E.C., Gaspar, V.M., Coutinho, P. e Correia, I.J. (2014) “Optimization of Liquid Overlay Technique to Formulate Heterogenic 3D Co-Cultures models”, Biotechnology and Bioengineering, Vol. 111 (8), pp. 1672-1685pt_PT
dc.identifier.doi10.1002/bit.25210pt_PT
dc.identifier.urihttp://hdl.handle.net/10400.6/4650
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherWileypt_PT
dc.relationBIOSYNTHESIS AND PURIFICATION OF MINICIRCLE DNA FOR APPLICATION IN DIABETES CELL-SPECIFIC GENE THERAPY
dc.relation.publisherversionhttps://onlinelibrary.wiley.com/doi/abs/10.1002/bit.25210pt_PT
dc.subjectCancerpt_PT
dc.subjectIn vitro modelspt_PT
dc.subjectLOTpt_PT
dc.subject3D MCTSpt_PT
dc.subjectTumor microenvironmentpt_PT
dc.titleOptimization of liquid overlay technique to formulate heterogenic 3D co‐cultures modelspt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleBIOSYNTHESIS AND PURIFICATION OF MINICIRCLE DNA FOR APPLICATION IN DIABETES CELL-SPECIFIC GENE THERAPY
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/FARH/SFRH%2FBD%2F80402%2F2011/PT
oaire.citation.endPage1685pt_PT
oaire.citation.startPage1672pt_PT
oaire.citation.titleBiotechnology and Bioengineeringpt_PT
oaire.citation.volume111pt_PT
oaire.fundingStreamFARH
person.familyNameda Rocha Costa
person.familyNameGaspar
person.familyNameTeixeira Gonçalves Coutinho Borges
person.familyNameJoaquim Sobreira Correia
person.givenNameElisabete Cristina
person.givenNameVítor
person.givenNamePaula Isabel
person.givenNameIlídio
person.identifierH-5423-2013
person.identifierUMbJ1KMAAAAJ
person.identifier.ciencia-idB314-4CF0-6633
person.identifier.ciencia-id6F16-3640-73E3
person.identifier.ciencia-id2311-7DBC-FA07
person.identifier.ciencia-idF610-7373-DC81
person.identifier.orcid0000-0002-0490-0095
person.identifier.orcid0000-0002-0372-2493
person.identifier.orcid0000-0001-6832-3082
person.identifier.orcid0000-0003-1613-9675
person.identifier.ridB-1602-2017
person.identifier.scopus-author-id55805850100
person.identifier.scopus-author-id36968590900
person.identifier.scopus-author-id36709479900
person.identifier.scopus-author-id7003557499
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.embargofctCopyright cedido à editora no momento da publicaçãopt_PT
rcaap.rightsclosedAccesspt_PT
rcaap.typearticlept_PT
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