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Abstract(s)
O presente trabalho encontra-se subdividido em dois capĂtulos que descrevem a
minha experiĂȘncia profissionalizante para obtenção do grau de Mestre em CiĂȘncias
FarmacĂȘuticas, apresentando uma componente de investigação, bem como uma
componente de estĂĄgio realizado em FarmĂĄcia ComunitĂĄria.
O primeiro capĂtulo, corresponde Ă componente de investigação e intitula-se
âEstudo do efeito de frequĂȘncias sonoras no crescimento de Escherichia coliâ.
Atualmente, sabe-se que Escherichia coli constitui o principal agente responsĂĄvel por
infeçÔes do trato urinĂĄrio. Este facto, aliado Ă crescente problemĂĄtica da resistĂȘncia aos
antibióticos, torna cada vez mais crucial a adequada seleção da antibioterapia com base
na suscetibilidade de cada microrganismo. No entanto, este processo leva,
habitualmente, 48 a 72 horas, o que torna evidente uma necessidade de inovação, de
forma a possibilitar a obtenção de um antibiograma de forma mais precoce. Sendo o
som uma perturbação fĂsica de origem vibratĂłria, este poderĂĄ possuir a capacidade de
produzir alteraçÔes no crescimento celular. Assim, foram selecionadas duas frequĂȘncias
sonoras (1.000Hz e 10.000Hz) para aplicação (durante 1 hora) nas diversas fases de
crescimento de E. coli, recorrendo a dois ensaios independentes, realizados em
duplicado. Verificou-se que o som possui, de facto, capacidade de induzir alteraçÔes no
crescimento celular, provocando um modesto aumento do mesmo, sendo que a fase de
latĂȘncia possui o maior interesse para aplicação do estĂmulo sonoro. No entanto, os
resultados obtidos nĂŁo apresentaram significĂąncia estatĂstica. Concluiu-se que sĂŁo
necessĂĄrios estudos futuros, utilizando, nomeadamente, novas frequĂȘncias, de modo a
otimizar a relação entre o estĂmulo sonoro e o incremento do crescimento celular.
O segundo capĂtulo ilustra as competĂȘncias adquiridas ao longo do estĂĄgio em
FarmĂĄcia ComunitĂĄria, realizado na FarmĂĄcia Avenida (Leiria), que se revelou fulcral
para a minha formação enquanto futura farmacĂȘutica. Neste capĂtulo, encontram-se
descritas as diversas vertentes em que assentam o papel do farmacĂȘutico comunitĂĄrio
em 2020, focando ainda o impacto que a pandemia Covid-19 provocou na farmĂĄcia.
This project is divided in two chapters, describing my professionalizing experience to obtain the masterâs degree in Pharmaceutical Sciences, consisting in an investigation component, as well as another part, which lays upon my internship in community pharmacy. The first chapter corresponds to the investigation part, named âStudy of the effect of sound frequencies on the growth of Escherichia coliâ. Nowadays, it is known that Escherichia coli is responsible for most of the urinary tract infections. This, along with the rising of antibiotic resistance rates, makes the need of selecting the adequate antibiotic therapy, based on the susceptibility of each specific bacteria, something essential. However, this process usually takes between 48 to 72 hours, making the need of innovation something that is evident, in order to obtain a microbiological diagnosis earlier. Sound, a physical disturbance of vibratory nature, could have the ability to induce changes in cellular growth. Therefore, two different sound frequencies were selected (1.000Hz and 10.000Hz) to apply (for 1 hour) on the different growth phases of E. coli, resorting to two independent essays, each in duplicate. It was found that sound stimulation could, in fact, induce changes in cellular growth, causing a modest growth increase, lag phase being the most interesting towards the application of the sound stimulus. Nevertheless, no statistically significant results were obtained. It was concluded that future studies are needed to optimize the relation between sound stimuli and cellular growth boost. The second chapter illustrates the skills that I acquired during my internship in community pharmacy, that took place at FarmĂĄcia Avenida (Leiria), which was fundamental in my training towards becoming a future pharmacist. In this chapter, the several spectra in which lays the role of the community pharmacist in 2020 are described, also mentioning the impact that the Covid-19 pandemic had on the pharmacy.
This project is divided in two chapters, describing my professionalizing experience to obtain the masterâs degree in Pharmaceutical Sciences, consisting in an investigation component, as well as another part, which lays upon my internship in community pharmacy. The first chapter corresponds to the investigation part, named âStudy of the effect of sound frequencies on the growth of Escherichia coliâ. Nowadays, it is known that Escherichia coli is responsible for most of the urinary tract infections. This, along with the rising of antibiotic resistance rates, makes the need of selecting the adequate antibiotic therapy, based on the susceptibility of each specific bacteria, something essential. However, this process usually takes between 48 to 72 hours, making the need of innovation something that is evident, in order to obtain a microbiological diagnosis earlier. Sound, a physical disturbance of vibratory nature, could have the ability to induce changes in cellular growth. Therefore, two different sound frequencies were selected (1.000Hz and 10.000Hz) to apply (for 1 hour) on the different growth phases of E. coli, resorting to two independent essays, each in duplicate. It was found that sound stimulation could, in fact, induce changes in cellular growth, causing a modest growth increase, lag phase being the most interesting towards the application of the sound stimulus. Nevertheless, no statistically significant results were obtained. It was concluded that future studies are needed to optimize the relation between sound stimuli and cellular growth boost. The second chapter illustrates the skills that I acquired during my internship in community pharmacy, that took place at FarmĂĄcia Avenida (Leiria), which was fundamental in my training towards becoming a future pharmacist. In this chapter, the several spectra in which lays the role of the community pharmacist in 2020 are described, also mentioning the impact that the Covid-19 pandemic had on the pharmacy.
Description
Keywords
Crescimento Celular Escherichia Coli FarmĂĄcia ComunitĂĄria FrequĂȘncias Sonoras