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Optical clearing methods: An overview of the techniques used for the imaging of 3D spheroids

2019-06-24Journal article Open access
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dc.contributor.authorCosta, Elisabete C
dc.contributor.authorSilva, Daniel N.
dc.contributor.authorMoreira, André
dc.contributor.authorCorreia, I.J.
dc.date.accessioned2019-08-26T09:20:34Z
dc.date.available2021-08-09T00:30:09Z
dc.date.issued2019-06-24
dc.description.abstractSpheroids have emerged as in vitro models that reproduce in a great extent the architectural microenvironment found in human tissues. However, the imaging of 3D cell cultures is highly challenging due to its high thickness, which results in a light-scattering phenomenon that limits light penetration. Therefore, several optical clearing methods, widely used in the imaging of animal tissues, have been recently explored to render spheroids with enhanced transparency. These methods are aimed to homogenize the microtissue refractive index (RI) and can be grouped into four different categories, namely (a) simple immersion in an aqueous solution with high RI; (b) delipidation and dehydration followed by RI matching; (c) delipidation and hyperhydration followed by RI matching; and (d) hydrogel embedding followed by delipidation and RI matching. In this review, the main optical clearing methods, their mechanism of action, advantages, and disadvantages are described. Furthermore, the practical examples of the optical clearing methods application for the imaging of 3D spheroids are highlighted.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationCosta EC, Silva DN, Moreira AF, Correia IJ. Optical clearing methods: An overview of the techniques used for the imaging of 3D spheroids. Biotechnology and Bioengineering. 2019;1–22.pt_PT
dc.identifier.doi10.1002/bit.27105pt_PT
dc.identifier.urihttp://hdl.handle.net/10400.6/7177
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherWileypt_PT
dc.relation3D multicellular spheroids as high throughput platforms to screen novel combinatory therapies for treatment of pancreatic cancer
dc.relationDevelopment of layer-by-layer polymeric microneedles for localized delivery of therapeutics to cervical cancer
dc.relation.publisherversionhttps://onlinelibrary.wiley.com/doi/epdf/10.1002/bit.27105pt_PT
dc.subjectImagingpt_PT
dc.subjectLight scatteringpt_PT
dc.subjectOptical clearingpt_PT
dc.subjectOptical microscopypt_PT
dc.subjectSpheroidspt_PT
dc.titleOptical clearing methods: An overview of the techniques used for the imaging of 3D spheroidspt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitle3D multicellular spheroids as high throughput platforms to screen novel combinatory therapies for treatment of pancreatic cancer
oaire.awardTitleDevelopment of layer-by-layer polymeric microneedles for localized delivery of therapeutics to cervical cancer
oaire.awardURIinfo:eu-repo/grantAgreement/FCT//SFRH%2FBD%2F103507%2F2014/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/POR_CENTRO/SFRH%2FBD%2F109482%2F2015/PT
oaire.citation.conferencePlaceEUApt_PT
oaire.citation.endPage22pt_PT
oaire.citation.startPage1pt_PT
oaire.citation.titleBiotechnology and Bioengineeringpt_PT
oaire.fundingStreamPOR_CENTRO
person.familyNameda Rocha Costa
person.familyNameMoreira
person.familyNameJoaquim Sobreira Correia
person.givenNameElisabete Cristina
person.givenNameAndré Ferreira
person.givenNameIlídio
person.identifier1161643
person.identifierUMbJ1KMAAAAJ
person.identifier.ciencia-idB314-4CF0-6633
person.identifier.ciencia-id4C1E-012B-83CE
person.identifier.ciencia-idF610-7373-DC81
person.identifier.orcid0000-0002-0490-0095
person.identifier.orcid0000-0002-0604-2506
person.identifier.orcid0000-0003-1613-9675
person.identifier.scopus-author-id55805850100
person.identifier.scopus-author-id56401521300
person.identifier.scopus-author-id7003557499
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.embargofctOs direitos do artigo foram cedidos à editorapt_PT
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
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