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Regucalcin is under-expressed in human breast and prostate cancers: Effect of sex steroid hormones

2009Journal article Restricted access
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dc.contributor.authorMaia, C J
dc.contributor.authorSantos, Cecília
dc.contributor.authorSchmitt, Fernando
dc.contributor.authorSocorro, Sílvia
dc.date.accessioned2019-11-26T17:13:57Z
dc.date.available2019-11-26T17:13:57Z
dc.date.issued2009
dc.description.abstractRegucalcin plays an important role in maintenance of intracellular Ca(2+) homeostasis, suppresses cell proliferation, inhibits expression of oncogenes, and increases the expression of tumour suppressor genes. This suggests that regucalcin functions may be altered in cancer tissues. In this study the regucalcin expression in breast and prostate cancer cases was analysed by RT-PCR and immunohistochemistry showing that the mRNA and/or protein are under-expressed in these tumors. The effect of sex steroid hormones on regucalcin expression in breast and prostate cancer cells was determined by real-time PCR. MCF-7 and LNCaP cells were stimulated with 0, 1, and 10 nM of 17beta-estradiol (E(2)) or 5alpha-dihydrotestosterone (DHT), respectively, for 0, 6, 12, 24, and 48 h. MCF-7 cells were also stimulated with E(2) conjugated to BSA (E(2)-BSA). To explore the mechanisms underlying the sex steroid regulation of regucalcin expression, control treatments with ICI 182,780, flutamide and cyclohexamide were carried out. E(2) effects regulating regucalcin expression were not abrogated in the presence of ICI 182,780, and were similar to those observed with E(2)-BSA, which suggests the involvement of a membrane-bound estrogen receptor. In LNCaP cells, DHT down-regulated regucalcin expression, an effect inhibited by the presence of both flutamide and cyclohexamide, suggesting the involvement of androgen receptor and de novo protein synthesis. The loss of regucalcin expression in breast and prostate cancer cases and the regulation of its expression by sex steroid hormones suggest that it may be associated with development and progression of these human tumors.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.doi10.1002/jcb.22158pt_PT
dc.identifier.urihttp://hdl.handle.net/10400.6/7622
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.subjectRegucalcinpt_PT
dc.subject17-beta-estradiolpt_PT
dc.subjectDHTpt_PT
dc.subjectSteroid hormonespt_PT
dc.subjectProstate cancerpt_PT
dc.titleRegucalcin is under-expressed in human breast and prostate cancers: Effect of sex steroid hormonespt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage676pt_PT
oaire.citation.issue4pt_PT
oaire.citation.startPage667pt_PT
oaire.citation.titleJournal of Cellular Biochemistrypt_PT
oaire.citation.volume107pt_PT
person.familyNameMaia Baptista
person.familyNameSANTOS
person.familyNameSchmitt
person.familyNameSocorro
person.givenNameCláudio Jorge
person.givenNameCecília Reis Alves
person.givenNameFernando
person.givenNameSílvia Cristina da Cruz Marques
person.identifierhttps://scholar.google.pt/citations?user=hFKOd_QAAAAJ&hl=pt-PT&oi=ao
person.identifierhttps://scholar.google.pt/citations?user=PA3L_h8AAAAJ&hl=pt-PT
person.identifier.ciencia-id5115-FCBD-A31F
person.identifier.ciencia-id761A-7ED2-D68F
person.identifier.ciencia-idAB17-5C25-1F2C
person.identifier.orcid0000-0002-5658-5445
person.identifier.orcid0000-0001-6074-7825
person.identifier.orcid0000-0003-1006-6946
person.identifier.orcid0000-0001-8181-488X
person.identifier.scopus-author-id23097426600
person.identifier.scopus-author-id7202545664
person.identifier.scopus-author-id23097994600
rcaap.embargofctCopyright cedido à editora no momento da publicaçãopt_PT
rcaap.rightsclosedAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublication1080c532-c737-4b60-bc44-1d18e3c877d7
relation.isAuthorOfPublication21e86061-71ea-4cfa-bc85-0fcd6e847911
relation.isAuthorOfPublication8dd07c9e-eb0d-4e4a-a8d1-2e3787e7f7bf
relation.isAuthorOfPublication1fb06949-0e6c-41a6-bb25-11885e7f0e9a
relation.isAuthorOfPublication.latestForDiscovery21e86061-71ea-4cfa-bc85-0fcd6e847911

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