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The biological performance of purified supercoiled p53 plasmid DNA in different cancer cell lines

dc.contributor.authorValente, J. F. A.
dc.contributor.authorSousa, A.
dc.contributor.authorGaspar, V. M.
dc.contributor.authorQueiroz, João
dc.contributor.authorFani, Sousa
dc.date.accessioned2020-01-09T17:00:11Z
dc.date.available2020-01-09T17:00:11Z
dc.date.issued2018
dc.description.abstractTumor suppressor p53 remains one of the most interesting therapeutic targets in cancer gene therapy due to itsconsistent mutation in numerous cancers. Thus, the reinstatement of the p53 expression and function can be seenas an effective alternative for cancer treatment, motivating research in thisfield. In this study,L-methioninematrix was used to purify the supercoiled topoisoform of a plasmid DNA encoding the p53 protein. This purebiopharmaceutical was conjugated with liposomes to comprehensively analyze itsin vitroperformance andtherapeutic potential in different cancer cell lines, including the lung and cervix models. A different profile ofcellular responses was attained after the transfection of these cancer cell lines with the p53-pDNA. Actually, thein vitrotransfection with pure sc p53-pDNA resulted in a higher expression of the tumor suppressor protein incancer cells when compared with the native pDNA samples (oc + sc topoisoforms). Also, wild-type p53 ex-pression following transfection was significantly higher in HeLa cervix cancer cells compared to that obtained inA549 lung cancer cells. Overall, ourfindings emphasize the potential of sc pDNA gene-based therapy, alsoraising awareness of the need to adjust the therapeutics, considering the feature of high heterogeneity of cancer cells.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.doi10.1016/j.procbio.2018.09.014pt_PT
dc.identifier.urihttp://hdl.handle.net/10400.6/8183
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherElsevierpt_PT
dc.relation.publisherversionhttps://www.sciencedirect.com/science/article/pii/S1359511318307554pt_PT
dc.subjectGene Therapypt_PT
dc.subjectL-methionine chromatographypt_PT
dc.subjectSupercoiled plasmid DNApt_PT
dc.subjectTumor suppressor p53pt_PT
dc.titleThe biological performance of purified supercoiled p53 plasmid DNA in different cancer cell linespt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/SFRH/SFRH%2FBD%2F96809%2F2013/PT
oaire.citation.endPage249pt_PT
oaire.citation.startPage240pt_PT
oaire.citation.titleProcess Biochemistrypt_PT
oaire.citation.volume75pt_PT
oaire.fundingStreamSFRH
person.familyNameValente
person.familyNameGaspar
person.familyNameQueiroz
person.familyNameSousa
person.givenNameJoana
person.givenNameVítor
person.givenNameJoão
person.givenNameFani
person.identifierVABrK9AAAAAJ
person.identifier.ciencia-id7515-7468-212D
person.identifier.ciencia-id6F16-3640-73E3
person.identifier.ciencia-id931E-B66D-E341
person.identifier.ciencia-id991D-2E13-A840
person.identifier.orcid0000-0002-2408-9762
person.identifier.orcid0000-0002-0372-2493
person.identifier.orcid0000-0002-3096-8325
person.identifier.orcid0000-0001-9996-2194
person.identifier.ridB-1602-2017
person.identifier.ridL-3104-2014
person.identifier.ridA-2014-2017
person.identifier.scopus-author-id36968590900
person.identifier.scopus-author-id7003705645
person.identifier.scopus-author-id7005110268
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.embargofctCopyright cedido à editora no momento da publicaçãopt_PT
rcaap.rightsclosedAccesspt_PT
rcaap.typearticlept_PT
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