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Abstract(s)
As plantas medicinais são um recurso cada vez mais procurado em diversas indústrias devido às suas propriedades antioxidantes, antimicrobianas e anti-inflamatórias. A Tuberaria lignosa (tradicionalmente conhecida como Erva Loba) é uma planta medicinal da família Cistaceae, rasteira e de flor amarela e está muito presente em pinhais e zonas áridas, nas regiões do Nordeste Português. As folhas da Tuberaria lignosa são uma fonte de compostos fenólicos, elagitaninos, flavonoides, açúcares e ácido ascórbico. Tem também uma ação inibitória da transcriptase reversa do HIV-1. A micropropagação de plantas é um processo importante para produzir plantas de interesse sem implicações ambientais. Desta forma é importante interligar métodos de análise de propriedades de interesse em plantas com a sua produção e comercialização de forma ininterrupta, rentável, sanificada e sem colocar em causa a extinção de espécies. Neste trabalho foram efetuadas sete tipos de extrações a partir de folhas frescas de Tuberaria lignosa (hidrodestilação, infusão, decocção, metanol 100%, etanol 96%, etanol 50% e n-Hexano) e uma a partir das flores (hidrodestilação), para posteriores testes antioxidantes (DPPH, Poder Redutor), Capacidade Redutora Total e antimicrobianos (difusão em disco e determinação dos valores da concentração mínima inibitória). Paralelamente foi iniciado um ensaio de multiplicação in vitro, pioneiro para esta espécie. Em conclusão, os extratos de Tuberaria lignosa demonstram ter propriedades interessantes sob o ponto de vista de atividade antioxidante, tendo as extrações aquosas demonstrado melhores resultados nos testes realizados. A nível antimicrobiano, o extrato da hidrodestilação das folhas apresenta um valor de MIC de 0.0195 mg/mL para a Candida albicans ATCC 90028 e para a Candida tropicalis ATCC 750, o que revela uma ligeira atividade sobre estas leveduras. O ensaio de multiplicação demonstrou que a luz LED tem uma forte influência no crescimento dos explantes e multiplicação da Tuberaria lignosa.
Medicinal plants are a resource increasingly sought in various industries due to its antioxidant, antimicrobial and anti-inflammatory properties. Tuberaria lignosa (traditionally known as Erva Loba) is a medicinal ground plant of the family Cistaceae presenting yellow flower, which is often present in pine forests and arid zones in the regions of Northeast of Portugal. Tuberaria lignosa leaves are a source of phenolic compounds, elagitanins, flavonoids, sugars and ascorbic acid. It also presents an inhibitory action on HIV-1 reverse transcriptase. Micropropagation of plants is an important process to produce plants of interest without environmental implications. In this way, it is important to interconnect methods of analysis of properties of interest in plants with their production in an uninterrupted, profitable and sanitized way, together with the prevention of the extinction of species. In this work, seven types of extractions were performed from fresh leaves of Tuberaria lignosa (hydrodistillation, infusion, decoction, 100% methanol, 96% ethanol, 50% ethanol and n-Hexane) and one from flowers (hydrodistillation) to perform antioxidant tests (DPPH, Reduction Power), Total Reducing Capacity and antimicrobial activity evaluation (disk diffusion and determination of minimum inhibitory concentration). In parallel, a pioneering in vitro multiplication assay was started for this species. In conclusion, Tuberaria lignosa showed interesting antioxidant properties, with aqueous extracts showing better results in the tests performed. Regarding the antimicrobial tests, leaves hydrodistillation extract has a MIC value of 0.0195 mg/mL for Candida albicans ATCC 90028 and for Candida tropicalis ATCC 750, which reveals a slight activity on these yeasts. The multiplication test demonstrated that LED light has a strong influence on explant growth and multiplication of Tuberaria lignosa.
Medicinal plants are a resource increasingly sought in various industries due to its antioxidant, antimicrobial and anti-inflammatory properties. Tuberaria lignosa (traditionally known as Erva Loba) is a medicinal ground plant of the family Cistaceae presenting yellow flower, which is often present in pine forests and arid zones in the regions of Northeast of Portugal. Tuberaria lignosa leaves are a source of phenolic compounds, elagitanins, flavonoids, sugars and ascorbic acid. It also presents an inhibitory action on HIV-1 reverse transcriptase. Micropropagation of plants is an important process to produce plants of interest without environmental implications. In this way, it is important to interconnect methods of analysis of properties of interest in plants with their production in an uninterrupted, profitable and sanitized way, together with the prevention of the extinction of species. In this work, seven types of extractions were performed from fresh leaves of Tuberaria lignosa (hydrodistillation, infusion, decoction, 100% methanol, 96% ethanol, 50% ethanol and n-Hexane) and one from flowers (hydrodistillation) to perform antioxidant tests (DPPH, Reduction Power), Total Reducing Capacity and antimicrobial activity evaluation (disk diffusion and determination of minimum inhibitory concentration). In parallel, a pioneering in vitro multiplication assay was started for this species. In conclusion, Tuberaria lignosa showed interesting antioxidant properties, with aqueous extracts showing better results in the tests performed. Regarding the antimicrobial tests, leaves hydrodistillation extract has a MIC value of 0.0195 mg/mL for Candida albicans ATCC 90028 and for Candida tropicalis ATCC 750, which reveals a slight activity on these yeasts. The multiplication test demonstrated that LED light has a strong influence on explant growth and multiplication of Tuberaria lignosa.
Description
Keywords
Antioxidantes Capacidade Redutora Total Difusão Em Disco Dpph Efeito Antimicrobiano Multiplicação In Vitro Poder Redutor Resazurina Tuberaria Lignosa