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Cholinium-Based Good’s Buffers Ionic Liquids as Remarkable Stabilizers and Recyclable Preservation Media for Recombinant Small RNAs

2018Journal article Restricted access
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dc.contributor.authorPedro, Augusto
dc.contributor.authorPereira, Patrícia
dc.contributor.authorQuental, Maria J.
dc.contributor.authorCarvalho, André P.
dc.contributor.authorSantos, Sérgio M.
dc.contributor.authorQueiroz, João
dc.contributor.authorSousa, Fani
dc.contributor.authorFreire, Mara G.
dc.date.accessioned2020-01-09T16:56:47Z
dc.date.available2020-01-09T16:56:47Z
dc.date.issued2018
dc.description.abstractRNA is a biopolymer of high relevance in the biopharmaceuticals field and in fundamental and applied research; however, the preservation of the RNA stability is still a remarkable challenge. Herein, we demonstrate the enhanced potential of aqueous solutions of self-buffering cholinium-based Good's buffers ionic liquids (GB-ILs), at 20 and 50 % (w/w), as alternative preservation media of recombinant small RNAs. The thermal stability of RNA is highly enhanced by GB-ILs, with an increase of 14 °C in the biopolymer melting temperature - the highest increase observed up to date with ILs. Most GB-ILs investigated improve the stability of RNA at least up to 30-days, both at 25 °C and at 4 °C, without requiring the typical samples freezing. Molecular dynamics simulations were applied to better understand the molecular-level mechanisms responsible for the observed RNA improved stability. The number of IL cations surrounding the RNA chain is similar, yet with differences found for the IL anions, which are responsible for the overall charge of the biopolymer first solvation sphere. No cytotoxicity of the studied solutions containing RNA and ILs at 20 % (w/w) was observed onto two distinct human cell lines, reinforcing their potential to act as preservation media when foreseeing biopharmaceutical applications. Finally, RNA was successfully recovered from the ILs aqueous solutions, without changes in its structural integrity, and the ILs successfully recycled and reused.pt_PT
dc.description.sponsorshipEuropean Research Council (ERC) for the Starting Grant ERC-2013-StG-337753pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.doi10.1021/acssuschemeng.8b03900pt_PT
dc.identifier.urihttp://hdl.handle.net/10400.6/8182
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherACS Publicationspt_PT
dc.relationStrategic Project - UI 709 - 2011-2012
dc.relation.publisherversionhttps://pubs.acs.org/doi/10.1021/acssuschemeng.8b03900pt_PT
dc.subjectNucleic acidspt_PT
dc.subjectRecombinant RNApt_PT
dc.subjectIonic liquidspt_PT
dc.subjectGood’s Bufferspt_PT
dc.subjectStabilitypt_PT
dc.subjectRecyclable mediapt_PT
dc.titleCholinium-Based Good’s Buffers Ionic Liquids as Remarkable Stabilizers and Recyclable Preservation Media for Recombinant Small RNAspt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleStrategic Project - UI 709 - 2011-2012
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/5876/PEst-OE%2FSAU%2FUI0709%2F2014/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/5876/UID%2FMulti%2F00709%2F2013/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6820 - DCRRNI ID/PEst-C%2FSAU%2FUI0709%2F2011/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/5876/UID%2FCTM%2F50011%2F2013/PT
oaire.citation.endPage16656pt_PT
oaire.citation.issue12pt_PT
oaire.citation.startPage16645pt_PT
oaire.citation.titleACS Sustainable Chemistry and Engineeringpt_PT
oaire.citation.volume6pt_PT
oaire.fundingStream5876
oaire.fundingStream5876
oaire.fundingStream6820 - DCRRNI ID
oaire.fundingStream5876
person.familyNameQuaresma Henriques Pedro
person.familyNamePereira
person.familyNameQueiroz
person.familyNameSousa
person.familyNameFreire
person.givenNameAugusto
person.givenNamePatrícia
person.givenNameJoão
person.givenNameFani
person.givenNameMara
person.identifierhttps://scholar.google.com/citations?user=oer44UoAAAAJ&hl=pt-PT&oi=ao
person.identifier.ciencia-id7111-B2BE-336D
person.identifier.ciencia-id4715-067B-4FD0
person.identifier.ciencia-id931E-B66D-E341
person.identifier.ciencia-id991D-2E13-A840
person.identifier.ciencia-id6718-1145-692F
person.identifier.orcid0000-0001-8194-6022
person.identifier.orcid0000-0002-5665-5271
person.identifier.orcid0000-0002-3096-8325
person.identifier.orcid0000-0001-9996-2194
person.identifier.orcid0000-0001-8895-0614
person.identifier.ridL-3104-2014
person.identifier.ridA-2014-2017
person.identifier.ridB-8458-2008
person.identifier.scopus-author-id54080426100
person.identifier.scopus-author-id56111844900
person.identifier.scopus-author-id7003705645
person.identifier.scopus-author-id7005110268
person.identifier.scopus-author-id7102299235
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.embargofctCopyright cedido à editora no momento da publicaçãopt_PT
rcaap.rightsclosedAccesspt_PT
rcaap.typearticlept_PT
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