Repository logo
 
Publication

Arcobacter butzleri Ciprofloxacin Resistance: Point Mutations in DNA Gyrase A and Role on Fitness Cost

2018Journal article Restricted access
Simple item page
dc.contributor.authorFerreira, Susana
dc.contributor.authorCorreia, Daniela R.
dc.contributor.authorOleastro, Mónica
dc.contributor.authorDomingues, F.C.
dc.date.accessioned2020-01-29T11:40:44Z
dc.date.available2020-01-29T11:40:44Z
dc.date.issued2018
dc.description.abstractArcobacter butzleri is a widely distributed emerging pathogen resistant to various classes of antimicrobial agents, namely fluoroquinolones. A. butzleri resistance to fluoroquinolones is conferred by point mutations at the antibiotic target. The aim of this study was to evaluate mutations at gyrA associated with ciprofloxacin resistance and evaluate whether acquisition of resistance impacts on fitness and stress tolerance of A. butzleri. A. butzleri ciprofloxacin mutants were generated by laboratory induction. Identification of mutations associated with ciprofloxacin resistance was performed by gyrA sequencing. Growth kinetics, cost of fitness, biofilm formation ability, and stress tolerance were assessed. Two amino acid substitutions in the quinolone resistance-determining region of GyrA were identified in the mutant strains, one previously described (Thr-85-Ile) and a new substitution (Asp-89-Tyr). No differences in growth kinetics were recorded between parental and mutant strains; however, fitness cost was variable, according to the genetic background of the strains, and independently of ciprofloxacin resistance. Overall, the ciprofloxacin resistance development did not significantly affect stress tolerance, motility, or biofilm-forming ability. In conclusion, acquisition of ciprofloxacin resistance in A. butzleri is associated with mutations in gyrA and is likely well compensated, with cost of fitness reflecting the diversity in genetic background of this bacterium.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.doi10.1089/mdr.2017.0295pt_PT
dc.identifier.urihttp://hdl.handle.net/10400.6/8879
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherMary Ann Liebertpt_PT
dc.relationStudies on the distribution and antibiotic resistance of Arcobacter
dc.relation.publisherversionhttps://www.liebertpub.com/doi/10.1089/mdr.2017.0295pt_PT
dc.subjectArcobacter butzleript_PT
dc.subjectCiprofloxacin resistancept_PT
dc.subjectDNA Gyrasept_PT
dc.subjectDrug Resistance - Bacterialpt_PT
dc.subjectFluoroquinolonespt_PT
dc.subjectPoint Mutationpt_PT
dc.subjectFitnesspt_PT
dc.subjectStress tolerancept_PT
dc.titleArcobacter butzleri Ciprofloxacin Resistance: Point Mutations in DNA Gyrase A and Role on Fitness Costpt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleStudies on the distribution and antibiotic resistance of Arcobacter
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/5876/UID%2FMulti%2F00709%2F2013/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT//SFRH%2FBPD%2F101959%2F2014/PT
oaire.citation.endPage922pt_PT
oaire.citation.issue7pt_PT
oaire.citation.startPage915pt_PT
oaire.citation.titleMicrobial Drug Resistancept_PT
oaire.citation.volume24pt_PT
oaire.fundingStream5876
person.familyNameFerreira
person.familyNameOleastro
person.familyNameDomingues
person.givenNameSusana
person.givenNameMónica
person.givenNameFernanda
person.identifier269435
person.identifier.ciencia-idF718-3569-E911
person.identifier.ciencia-id4115-04A9-FBCB
person.identifier.ciencia-id6514-0498-3E8F
person.identifier.orcid0000-0001-8308-2862
person.identifier.orcid0000-0001-6360-2576
person.identifier.orcid0000-0002-9540-0853
person.identifier.ridA-6810-2016
person.identifier.ridN-7295-2013
person.identifier.scopus-author-id34874668400
person.identifier.scopus-author-id6603067224
person.identifier.scopus-author-id10139481400
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.embargofctCopyright cedido à editora no momento da publicaçãopt_PT
rcaap.rightsclosedAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublication9f4d39d4-7d9c-4c17-8a8a-523d32d4de20
relation.isAuthorOfPublicationa88128e4-2c67-4a98-afd4-64cc3775dcc4
relation.isAuthorOfPublication8d5fe6e2-21af-4557-9c46-84103254a18a
relation.isAuthorOfPublication.latestForDiscovery9f4d39d4-7d9c-4c17-8a8a-523d32d4de20
relation.isProjectOfPublicationcb4688cf-40da-4197-9eb0-c55bad699ad3
relation.isProjectOfPublication1678a975-212c-45a7-a9ba-d2b2015c7824
relation.isProjectOfPublication.latestForDiscoverycb4688cf-40da-4197-9eb0-c55bad699ad3

Files

Original bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
2018. Arcobacter butzleri Ciprofloxacin Resistance- Point Mutations in DNA Gyrase A and Role on Fitness Cost.pdf
Size:
347.83 KB
Format:
Adobe Portable Document Format
Download
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
1.71 KB
Format:
Item-specific license agreed upon to submission
Description:
Download

Collections

ICI - CICS | Documentos por Auto-Depósito