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Abstract(s)
Introdução: O octilmetoxicinamato é um dos filtros de radiação ultravioleta B mais utilizados
em protetores solares e produtos cosméticos. Após alguns estudos in vitro e in vivo, há a
suspeita de que possa atuar como disruptor endócrino, com possível atividade estrogénica e
anti-tiroideia. É absorvido pela pele, sendo detetado no plasma depois da sua aplicação tópica.
Também foi detetado em amostras do leite materno humano. Dada a sua natureza lipofílica,
tende a acumular-se no ambiente e foi considerado uma substância de grande preocupação
relativamente ao seu risco para a saúde. Ainda não foram elaborados estudos de modo a avaliar
a sua ação no sistema cardiovascular, mas pensa-se que poderá ter efeitos semelhantes aos do
estrogénio. A sua ação no músculo liso pode ser desencadeada por vias genómicas e não
genómicas que podem ser dependentes ou independentes do endotélio.
Objetivo: Avaliar os efeitos do octilmetoxicinamato no músculo liso das artérias umbilicais
humanas sem endotélio, de modo a analisar os seus possíveis efeitos não genómicos
independentes do endotélio, e se estes se assemelham aos do estrogénio, a nível vascular.
Material e Métodos: Utilizando a técnica de banho de órgãos, anéis de artérias umbilicais
humanas sem endotélio foram contraídas com serotonina 1µM e cloreto de potássio 60mM. O
efeito do octilmetoxicinamato a diferentes concentrações foi analisado. Todos os
procedimentos realizados foram aprovados pelo Comité de ética do Centro Hospitalar da Cova
da Beira E.P.E.
Resultados: Observou-se um efeito um efeito de vasorelaxamento, nas artérias umbilicais
humanas sem endotélio contraídas com serotonina 1µM e com cloreto de potássio 60mM.
Ocorreu um relaxamento semelhante entre os dois agentes contráteis nas diferentes
concentrações, com efeito máximo na concentração 5x10-5M, para os dois agentes contracteis
analisados.
Discussão: O octilmetoxicinamato poderá desencadear o vasorelaxamento, de uma forma
semelhante ao 17ß-Estradiol, por um mecanismo não genómico e independente do endotélio.
Não existem estudos publicados relativamente ao efeito do octilmetoxicinamato a nível
vascular, bem como o seu possível papel como disruptor endócrino e no desenvolvimento de
patologias cardiovasculares. O presente estudo foi pioneiro nesse sentido. São necessários
estudos adicionais de modo a averiguar se o seu mecanismo de ação está relacionado com a
ativação do recetor GPR30. Conclusão: O octilmetoxicinamato tem um efeito não genómico vasorelaxante em artérias
umbilicais humanas através de um mecanismo independente do endotélio.
Introduction: Octyl-methoxycinnamate is one of the most commonly used ultraviolet B radiation filters in sunscreens and other cosmetic products. After some in vitro e in vivo studies, it is suspected that it can act as an endocrine disruptor, possibly with estrogenic and antithyroid actions. It is absorbed through the skin and can be detected in the plasma after topical application. It is detected in human milk samples as well. It is a lipophilic molecule, tends to bioaccumulate in the environment, and it was considered as a substance of high concern in relation to human risk and health. There is no studies evaluating the action of octylmethoxycinnamate on the cardiovascular system but there is a possibility that it can have similar effects to those of estrogens in different arteries. It’s action on vascular smooth muscle cells can be trigged through genomic and non-genomic pathways that can be dependent or independent of the endothelium. Objective: Assess octyl-methoxycinnamate’s effects on the smooth muscle of human umbilical arteries without endothelium in order to analyze its potential non-genomic effects independent of endothelium, and see if they are similar to those of estrogen at a vascular level. Material and Methods: Using organ bath techniques, rings of human umbilical arteries without endothelium were contracted by serotonin 1µM and potassium chloride 60mM. The effects of octyl-methoxycinnamate were analyzed at different concentrations. All procedures were approved by the ethics committee of Cova da Beira’s Hospital Center. Results: Octyl-methoxycinnamate relaxed human umbilical arteries without endothelium contracted with both serotonin 1µM and potassium chloride 60mM. There was a similar vasorelaxant effect between the two contractile agents at different, with maximum effect at 5x10-5M, in both contractile agents. Discussion: Octyl-methoxycinnamate could trigger a vasorelaxant effect in a similar way that of 17ß-Estradiol, through a non-genomic and endothelium independent mechanism. There isn’t published data regarding octyl-methoxycinnamate effects at a vascular level, as well as it’s possible action as an endocrine disruptor and in the development of cardiovascular diseases. The present study is a pioneer in that way. It is necessary more data to evaluate if the mechanism of action of octyl-methoxycinnamate is related with the activation of the receptor GPR30. Conclusion: Octyl-methoxycinnamate has a non-genomic vasorelaxant effect in human umbilical arteries through an endothelium independent mechanism.
Introduction: Octyl-methoxycinnamate is one of the most commonly used ultraviolet B radiation filters in sunscreens and other cosmetic products. After some in vitro e in vivo studies, it is suspected that it can act as an endocrine disruptor, possibly with estrogenic and antithyroid actions. It is absorbed through the skin and can be detected in the plasma after topical application. It is detected in human milk samples as well. It is a lipophilic molecule, tends to bioaccumulate in the environment, and it was considered as a substance of high concern in relation to human risk and health. There is no studies evaluating the action of octylmethoxycinnamate on the cardiovascular system but there is a possibility that it can have similar effects to those of estrogens in different arteries. It’s action on vascular smooth muscle cells can be trigged through genomic and non-genomic pathways that can be dependent or independent of the endothelium. Objective: Assess octyl-methoxycinnamate’s effects on the smooth muscle of human umbilical arteries without endothelium in order to analyze its potential non-genomic effects independent of endothelium, and see if they are similar to those of estrogen at a vascular level. Material and Methods: Using organ bath techniques, rings of human umbilical arteries without endothelium were contracted by serotonin 1µM and potassium chloride 60mM. The effects of octyl-methoxycinnamate were analyzed at different concentrations. All procedures were approved by the ethics committee of Cova da Beira’s Hospital Center. Results: Octyl-methoxycinnamate relaxed human umbilical arteries without endothelium contracted with both serotonin 1µM and potassium chloride 60mM. There was a similar vasorelaxant effect between the two contractile agents at different, with maximum effect at 5x10-5M, in both contractile agents. Discussion: Octyl-methoxycinnamate could trigger a vasorelaxant effect in a similar way that of 17ß-Estradiol, through a non-genomic and endothelium independent mechanism. There isn’t published data regarding octyl-methoxycinnamate effects at a vascular level, as well as it’s possible action as an endocrine disruptor and in the development of cardiovascular diseases. The present study is a pioneer in that way. It is necessary more data to evaluate if the mechanism of action of octyl-methoxycinnamate is related with the activation of the receptor GPR30. Conclusion: Octyl-methoxycinnamate has a non-genomic vasorelaxant effect in human umbilical arteries through an endothelium independent mechanism.
Description
Keywords
Octilmetoxicinamato · Disruptor Endócrino · Artéria Umbilical Humana · Estrogénio · Gpr30