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Quorum sensing inhibition evaluation method: An experiment-based microbiology laboratory course

2024-12-12Journal article Restricted access
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datacite.subject.fosCiências Médicas
datacite.subject.sdg03:Saúde de Qualidade
dc.contributor.authorLuís, Ângelo Filipe Santos
dc.contributor.authorDomingues, Fernanda
dc.date.accessioned2025-08-22T11:17:13Z
dc.date.available2025-08-22T11:17:13Z
dc.date.issued2024-12-12
dc.description.abstractBacteria have developed a cell-to-cell communication system called quorum sensing (QS), allowing them to regulate group behavior and synchronize the expression of virulence factors, responsible for increasing their infection capacity and resistance to antimicrobials. Although the control of microbial infections through the inhibition of microbial growth has traditionally been the basis of antimicrobial chemotherapy, the emergence of antimicrobial resistance has led to the search for new microbial control strategies, namely through the inhibition of QS. Among the agents studied to inhibit this bacterial communication are essential oils (EO), which are considered very effective QS inhibitors. When searching for new QS inhibitor agents, it is essential to have a cheap and easy-to-perform method that allows the evaluation of this activity. Chromobacterium violaceum is a Gram-negative bacterium that has been widely used as a model organism in QS research laboratories because it produces the violet-colored pigment violacein, which is regulated by QS and is an easily observable and quantifiable characteristic marker. The objective of this work is to describe a method to evaluate the inhibition of the QS using Cymbopogon martinii EO as a potential inhibitory agent for violacein production by C. violaceum, which can be applied in the Microbiology laboratory course as a part of the programs of several science degrees. The proposed method is inexpensive and does not require specific equipment, enabling its easy implementation by the laboratory team and professors.eng
dc.identifier.doi10.1002/bmb.21874
dc.identifier.urihttp://hdl.handle.net/10400.6/18915
dc.language.isoeng
dc.peerreviewedyes
dc.publisherInternational Union of Biochemistry and Molecular Biology (IUBMB)
dc.relationHealth Sciences Research Centre
dc.relationNot Available
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/
dc.subjectChromobacterium Violaceum
dc.subjectCymbopogon Martinii
dc.subjectEssential Oil
dc.subjectMicrobiology Course
dc.subjectQuorum Sensing
dc.titleQuorum sensing inhibition evaluation method: An experiment-based microbiology laboratory courseeng
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleHealth Sciences Research Centre
oaire.awardTitleNot Available
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F00709%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/DL 57%2F2016/DL 57%2F2016%2FCP1354%2FCT0010/PT
oaire.citation.titleBiochemistry and Molecular Biology Education
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStreamDL 57/2016
oaire.versionhttp://purl.org/coar/version/c_970fb48d4fbd8a85
person.familyNameLuís
person.familyNameDomingues
person.givenNameÂngelo Filipe Santos
person.givenNameFernanda
person.identifier.ciencia-id3D14-798B-65D3
person.identifier.ciencia-id6514-0498-3E8F
person.identifier.orcid0000-0003-0712-6522
person.identifier.orcid0000-0002-9540-0853
person.identifier.ridN-7295-2013
person.identifier.scopus-author-id10139481400
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
relation.isAuthorOfPublicationa2d7c872-99db-41e1-9bc9-82c2d47b4661
relation.isAuthorOfPublication8d5fe6e2-21af-4557-9c46-84103254a18a
relation.isAuthorOfPublication.latestForDiscoverya2d7c872-99db-41e1-9bc9-82c2d47b4661
relation.isProjectOfPublicationc7cee677-067c-47b2-ab19-b2dd5b0fda9d
relation.isProjectOfPublicatione4a98845-fa46-4626-b118-1ba89fd7067f
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