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HPV-16 targeted DNA vaccine expression: The role of purification

2018Journal article Restricted access
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dc.contributor.authorAlmeida, Ana Margarida
dc.contributor.authorTomás, Joana
dc.contributor.authorPereira, Patrícia
dc.contributor.authorQueiroz, João
dc.contributor.authorSousa, Fani
dc.contributor.authorSousa, Ângela
dc.date.accessioned2020-01-09T16:36:21Z
dc.date.available2020-01-09T16:36:21Z
dc.date.issued2018
dc.description.abstractDNA vaccines have come to light in the last decades as an alternative method to prevent many infectious diseases, but they can also be used for the treatment of specific diseases, such as cervical cancer caused by Human Papillomavirus (HPV). This virus produces E6 and E7 oncoproteins, which alter the cell cycle regulation and can interfere with the DNA repairing system. These features can ultimately lead to the progression of cervical cancer, after cell infection by HPV. Thus, the development of a DNA vaccine targeting both proteins arises as an interesting option in the treatment of this pathology. Nonetheless, before evaluating its therapeutic potential, the purity levels of a biopharmaceutical must meet the regulatory agency specifications. Previously, our research group successfully purified the supercoiled isoform of the recombinant HPV-16 E6/E7 DNA vaccine with virtual 100% purity by affinity chromatography. The present work was designed to evaluate the effect that pDNA sample purity levels may exert in the expression of a target protein. Thus, in vitro studies were performed to assess the vaccine ability to produce the target proteins and to compare the expression efficiency between the pDNA sample obtained by affinity chromatography, which only presents the sc isoform and fulfils the regulatory agency recommendations, and the same DNA vaccine retrieved by a commercial purification kit, which contains different pDNA isoforms. Our achievements suggest that the E6/E7 DNA vaccine purified by affinity chromatography promotes higher E6 and E7 mRNA and protein expression levels than the DNA vaccine purified with the commercial kit. Overall, these results underline the importance that a purification strategy may present in the therapeutic outcome of recombinant DNA vaccines, envisaging their further application as biopharmaceuticals. © 2018 American Institute of Chemical Engineers Biotechnol. Prog., 34:546-551, 2018.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.doi10.1002/btpr.2603pt_PT
dc.identifier.urihttp://hdl.handle.net/10400.6/8179
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherAmerican Institute of Chemical Engineerspt_PT
dc.relationBiosynthesis and purification of minicircle DNA encoding pri-miR-375 for treatment of Human Papillomavirus infection
dc.relation.publisherversionhttps://aiche.onlinelibrary.wiley.com/doi/abs/10.1002/btpr.2603pt_PT
dc.subjectCancer Vaccinespt_PT
dc.subjectDNA Repairpt_PT
dc.subjectFemalept_PT
dc.subjectHuman papillomavirus 16pt_PT
dc.subjectHumanspt_PT
dc.subjectOncogene Proteins - Viralpt_PT
dc.subjectPapillomavirus E7 Proteinspt_PT
dc.subjectRepressor Proteinspt_PT
dc.subjectUterine Cervical Neoplasmspt_PT
dc.subjectVaccines - DNApt_PT
dc.titleHPV-16 targeted DNA vaccine expression: The role of purificationpt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleBiosynthesis and purification of minicircle DNA encoding pri-miR-375 for treatment of Human Papillomavirus infection
oaire.awardURIinfo:eu-repo/grantAgreement/FCT//SFRH%2FBD%2F102284%2F2014/PT
oaire.citation.endPage551pt_PT
oaire.citation.issue2pt_PT
oaire.citation.startPage546pt_PT
oaire.citation.titleBiotechnology Progresspt_PT
oaire.citation.volume34pt_PT
person.familyNameAlmeida
person.familyNameMelfe Tomás
person.familyNamePereira
person.familyNameQueiroz
person.familyNameSousa
person.familyNameSousa
person.givenNameAna Margarida
person.givenNameJoana Filipa
person.givenNamePatrícia
person.givenNameJoão
person.givenNameFani
person.givenNameÂngela
person.identifier817754
person.identifier.ciencia-idF51D-5365-D95D
person.identifier.ciencia-id4715-067B-4FD0
person.identifier.ciencia-id931E-B66D-E341
person.identifier.ciencia-id991D-2E13-A840
person.identifier.ciencia-idFB1F-08DE-23FE
person.identifier.orcid0000-0002-9313-6649
person.identifier.orcid0000-0001-8796-5152
person.identifier.orcid0000-0002-5665-5271
person.identifier.orcid0000-0002-3096-8325
person.identifier.orcid0000-0001-9996-2194
person.identifier.orcid0000-0001-9155-7581
person.identifier.ridL-3104-2014
person.identifier.ridA-2014-2017
person.identifier.scopus-author-id56111844900
person.identifier.scopus-author-id7003705645
person.identifier.scopus-author-id7005110268
person.identifier.scopus-author-id24330268800
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.embargofctCopyright cedido à editora no momento da publicaçãopt_PT
rcaap.rightsclosedAccesspt_PT
rcaap.typearticlept_PT
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