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8-(3-phenylpropyl)-1,3,7-triethylxanthine is a synthetic caffeine substitute with stronger metabolic modulator activity

2018Journal article Open access
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dc.contributor.authorCarrageta, David F
dc.contributor.authorDias, Tânia R.
dc.contributor.authorJarak, Ivana
dc.contributor.authorAlves, Marco G
dc.contributor.authorOliveira, P.F.
dc.contributor.authorWalt, Mietha Van Der
dc.contributor.authorTerre'Blanche, Gisella
dc.contributor.authorMonteiro, Mariana P.
dc.contributor.authorSilva, Branca M.
dc.date.accessioned2020-02-17T10:08:52Z
dc.date.available2020-02-17T10:08:52Z
dc.date.issued2018
dc.description.abstractCaffeine is one of the most worldwide consumed methylxanthines. It is well-known for its thermogenic and cell metabolism modulating effects. Based on methylxanthines' chemical structure, 8-(3-phenylpropyl)-1,3,7-triethylxanthine (PTX) is a novel adenosine antagonist with higher receptor affinity than caffeine. Therefore, we hypothesized that PTX metabolic effects could be stronger than those of caffeine. For that purpose, murine 3T3-L1 cells were cultured in the presence of increasing doses of PTX or caffeine (0.1, 1, 10 and 100 μM) for 24 h. Cytotoxicity was evaluated by reduction of tetrazolium salt (MTT) and lactate dehydrogenase (LDH) release. Cell metabolites released to the culture medium were identified and quantified by proton nuclear magnetic resonance (1H NMR). Cellular oxidative profile was also evaluated. Our results showed that PTX displayed no signs of cytotoxicity at all studied concentrations. When compared with caffeine, PTX increased glucose, pyruvate, and glutamine consumption, as well as lactate, alanine, and acetate production. Additionally, PTX decreased protein oxidation, thus protecting against oxidative stress-induced damage. These results illustrate that PTX is a stronger and less cytotoxic caffeine substitute with potential applications as metabolic modulator and a good candidate for novel drug design.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.doi10.1016/j.tiv.2018.08.002pt_PT
dc.identifier.urihttp://hdl.handle.net/10400.6/9279
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.relationWhite tea WTEA as a promising agent against sufertility/infetility promoted by diabetes mellitus: synesgistic or single component effect
dc.relationObesitility – Gut hormones and testicular metabolism: mechanisms associated with obesity-related male infertility
dc.subject3T3-L1 Cellspt_PT
dc.subjectAnimalspt_PT
dc.subjectCell Survivalpt_PT
dc.subjectGlycolysispt_PT
dc.subjectL-Lactate Dehydrogenasept_PT
dc.subjectMicept_PT
dc.subjectOxidation-Reductionpt_PT
dc.subjectXanthinespt_PT
dc.title8-(3-phenylpropyl)-1,3,7-triethylxanthine is a synthetic caffeine substitute with stronger metabolic modulator activitypt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleWhite tea WTEA as a promising agent against sufertility/infetility promoted by diabetes mellitus: synesgistic or single component effect
oaire.awardTitleObesitility – Gut hormones and testicular metabolism: mechanisms associated with obesity-related male infertility
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/POR_NORTE/SFRH%2FBD%2F109284%2F2015/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/5876/PEst-OE%2FSAU%2FUI0215%2F2014/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/9471 - RIDTI/PTDC%2FBIM-MET%2F4712%2F2014/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/9471 - RIDTI/PTDC%2FBBB-BQB%2F1368%2F2014/PT
oaire.citation.endPage120pt_PT
oaire.citation.startPage114pt_PT
oaire.citation.titleToxicology in Vitropt_PT
oaire.citation.volume53pt_PT
oaire.fundingStreamPOR_NORTE
oaire.fundingStream5876
oaire.fundingStream9471 - RIDTI
oaire.fundingStream9471 - RIDTI
person.familyNameCarrageta
person.familyNameDias
person.familyNameJarak
person.familyNameAlves
person.familyNameOliveira
person.familyNameVan der Walt
person.familyNameTerre'Blanche
person.familyNameMonteiro
person.familyNameSilva
person.givenNameDavid
person.givenNameTânia
person.givenNameIvana
person.givenNameMarco G.
person.givenNamePedro Fontes
person.givenNameMietha Magdalena
person.givenNameGisella
person.givenNameMariana
person.givenNameBranca Maria
person.identifierR-00H-H65
person.identifier2358493
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person.identifier.orcid0000-0002-1518-3925
person.identifier.orcid0000-0001-5586-3071
person.identifier.orcid0000-0002-0662-1831
person.identifier.orcid0000-0002-5610-8024
person.identifier.ridG-1183-2018
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person.identifier.ridD-2715-2019
person.identifier.scopus-author-id55619567500
person.identifier.scopus-author-id8383665600
person.identifier.scopus-author-id57202054274
person.identifier.scopus-author-id36198848500
person.identifier.scopus-author-id7801438801
person.identifier.scopus-author-id12243571300
person.identifier.scopus-author-id7005615589
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
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