Loading...
Publication
Análise da influência dos genótipos do gene GSTT1 no desenvolvimento de lesão por HPV
| Name: | Description: | Size: | Format: | |
|---|---|---|---|---|
| 823.86 KB | Adobe PDF |
Authors
Abstract(s)
O cancro do colo do útero é um dos cancros mais comuns na população feminina. Esta
neoplasia pode desenvolver-se a partir de lesões intraepiteliais que podem ter origem em
infeções persistentes por HPV de alto risco. Estas infeções se não forem detetadas e
tratadas precocemente, podem evoluir para cancro do colo do útero.
O vírus do papiloma humano é um vírus de ADN que pertence à família Papillomaviridae,
sendo um dos vírus sexualmente mais transmissíveis. O HPV tem mais de 200 subtipos
podendo ser dividido em grupos de alto e de baixo risco, estando os grupos de alto risco
mais relacionados com infeções persistentes e o desenvolvimento de patologias.
Neste trabalho pretendeu-se estudar a presença e a influência do genótipo “null” do GSTT1
no desenvolvimento de lesão por HPV. A GSTT1 pertence a uma família de enzimas de fase
II que atuam no metabolismo xenobiótico conjugando metabolitos com a glutationa
tornando-os mais hidrossolúveis e consequentemente reduzindo o efeito citotóxico.
Foram utilizadas amostras de tecido da cérvix uterina de 45 mulheres, das quais se extraiu
ADN genómico para posteriormente se identificar a estirpe de HPV através de PCR. A
determinação do genótipo do GSTT1 foi realizada por Real-Time PCR. Das 45 amostras
utilizadas, em 22 foi possível identificar o genótipo “null” do GSTT1. Na população em
estudo, parece não existir relação entre o polimorfismo “null” do gene GSTT1 e o
desenvolvimento de lesão por HPV.
Atualmente, na literatura existem ainda poucos estudos relacionados a este tema.
Investigações futuras poderão vir a clarificar o papel do genótipo “null” do GSTT1 no
desenvolvimento de lesão por HPV.
Cervical cancer is one of the most common cancers among women. This neoplasm can develop from intraepithelial lesions that may originate from persistent high-risk HPV infections. These infections, if not detected and treated early, can progress to cervical cancer. The human papilloma virus is a DNA virus that belongs to the Papillomaviridae family and is one of the most sexually transmitted viruses. HPV has more than 200 subtypes and can be divided into high and low risk groups, with high risk groups more related to persistent infections and the development of pathologies. The aim of this work was to study the presence and influence of the GSTT1 “null” genotype on the development of HPV lesions. GSTT1 belongs to a family of phase II enzymes that act in xenobiotic metabolism by conjugating metabolites with glutathione, making them more water soluble and consequently reducing the cytotoxic effect. Tissue samples from the uterine cervix of 45 women were used, from which genomic DNA was extracted in order to subsequently identify the HPV strain through PCR. The determination of GSTT1 genotype was performed by Real-Time PCR. Of the 45 samples used, in 22 it was possible to identify the “null” genotype of GSTT1. In the studied population, there seems to be no relationship between the “null” polymorphism of the GSTT1 gene and the development of lesions caused by HPV. Currently, there are still few studies related to this topic in the literature. Future investigations may clarify the role of the GSTT1 “null” genotype in the development of HPV lesions.
Cervical cancer is one of the most common cancers among women. This neoplasm can develop from intraepithelial lesions that may originate from persistent high-risk HPV infections. These infections, if not detected and treated early, can progress to cervical cancer. The human papilloma virus is a DNA virus that belongs to the Papillomaviridae family and is one of the most sexually transmitted viruses. HPV has more than 200 subtypes and can be divided into high and low risk groups, with high risk groups more related to persistent infections and the development of pathologies. The aim of this work was to study the presence and influence of the GSTT1 “null” genotype on the development of HPV lesions. GSTT1 belongs to a family of phase II enzymes that act in xenobiotic metabolism by conjugating metabolites with glutathione, making them more water soluble and consequently reducing the cytotoxic effect. Tissue samples from the uterine cervix of 45 women were used, from which genomic DNA was extracted in order to subsequently identify the HPV strain through PCR. The determination of GSTT1 genotype was performed by Real-Time PCR. Of the 45 samples used, in 22 it was possible to identify the “null” genotype of GSTT1. In the studied population, there seems to be no relationship between the “null” polymorphism of the GSTT1 gene and the development of lesions caused by HPV. Currently, there are still few studies related to this topic in the literature. Future investigations may clarify the role of the GSTT1 “null” genotype in the development of HPV lesions.
Description
Keywords
Cancro do Colo do Útero Gstt1 Hpv