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Authors
Abstract(s)
Um laboratório clínico é um local específico para análise de produtos provenientes do corpo
humano, com o propósito de fornecer informações para o diagnóstico, prevenção ou
tratamento de qualquer doença. A bioquímica é elementar para a prática da medicina clínica
e um dos objetivos de qualquer laboratório é assegurar que esses dados estejam disponíveis
e que sejam obtidos eficientemente. O presente relatório retrata a passagem nos diferentes
setores do laboratório de Patologia Clínica do Centro Hospitalar Tondela-Viseu, focando
mais os setores de microbiologia e hematologia.
Novos fatores de resistência aparecem regularmente e as populações bacterianas resistentes
espalham-se quando os antibióticos exercem pressões seletivas que favorecem a resistência.
Os antibióticos podem eliminar populações microbianas suscetíveis, reduzindo a
competição e expandindo os recursos disponíveis para bactérias resistentes. A bactéria
Escherichia coli tem um lugar especial no mundo microbiológico, pois pode causar infeções
graves em humanos e animais, mas também representa uma parte significativa da
microbiota autóctone dos diferentes hospedeiros. Um dos antibióticos usados no
tratamento é a fosfomicina pois interfere na síntese da parede celular, bloqueando a
primeira etapa da síntese do peptidoglicano. O principal objetivo deste projeto de
investigação foi avaliar a utilidade do sistema comercial automatizado de microdiluição em
caldo – VITEK® 2 em estudos de rotina de modo a detetar a suscetibilidade de E. coli à
fosfomicina, comparando-a com o método de difusão em disco, designado como método de
Kirby-Bauer. Apesar do pequeno número de isolados resistentes, pode verificar-se que os
resultados apresentados pelo VITEK® 2 e os resultados obtidos pelo método da difusão em
disco não constituíram matéria de discordância durante o estudo, demonstrando assim a
possibilidade de se adotar em rotina apenas o método automático para o estudo de
fosfomicina em infeções por E. coli.
A clinical laboratory is a specific place for analyzing products from the human body in order to provide information for the diagnosis, prevention or treatment of any disease. Biochemistry is elemental to the practice of clinical medicine, and one of the goals of any laboratory is to ensure that this data is available and that it is obtained efficiently. This report portrays the passage in the different sectors of the Clinical Pathology laboratory of Centro Hospitalar Tondela-Viseu, focusing more on the microbiology and hematology sectors. New resistance factors appear regularly, and resistant bacterial populations spread when antibiotics exert selective pressures that favor resistance. Antibiotics can eliminate susceptible microbial populations, reducing competition and expanding the resources available to resistant bacteria. The bacterium Escherichia coli has a special place in the microbiological world, as it can cause serious infections in humans and animals, but also represents a significant part of the indigenous microbiota of different hosts. One of the antibiotics used for treatment is fosfomycin because it interferes with cell wall synthesis by blocking the first step of peptidoglycan synthesis., The main objective of this research project was to evaluate the usefulness of the commercial automated broth microdilution system – VITEK® 2 in routine studies to detect the susceptibility of E. coli to fosfomycin, comparing it with the disk diffusion method, referred to as the Kirby-Bauer method. Despite the small number of resistant isolates, it can be verified that the results presented by the VITEK® 2 and the results obtained by the disk diffusion method showed agreement during the study, thus demonstrating the possibility of routinely adopting only the automated method for the study of fosfomycin in E. coli infections.
A clinical laboratory is a specific place for analyzing products from the human body in order to provide information for the diagnosis, prevention or treatment of any disease. Biochemistry is elemental to the practice of clinical medicine, and one of the goals of any laboratory is to ensure that this data is available and that it is obtained efficiently. This report portrays the passage in the different sectors of the Clinical Pathology laboratory of Centro Hospitalar Tondela-Viseu, focusing more on the microbiology and hematology sectors. New resistance factors appear regularly, and resistant bacterial populations spread when antibiotics exert selective pressures that favor resistance. Antibiotics can eliminate susceptible microbial populations, reducing competition and expanding the resources available to resistant bacteria. The bacterium Escherichia coli has a special place in the microbiological world, as it can cause serious infections in humans and animals, but also represents a significant part of the indigenous microbiota of different hosts. One of the antibiotics used for treatment is fosfomycin because it interferes with cell wall synthesis by blocking the first step of peptidoglycan synthesis., The main objective of this research project was to evaluate the usefulness of the commercial automated broth microdilution system – VITEK® 2 in routine studies to detect the susceptibility of E. coli to fosfomycin, comparing it with the disk diffusion method, referred to as the Kirby-Bauer method. Despite the small number of resistant isolates, it can be verified that the results presented by the VITEK® 2 and the results obtained by the disk diffusion method showed agreement during the study, thus demonstrating the possibility of routinely adopting only the automated method for the study of fosfomycin in E. coli infections.
Description
Keywords
Difusão
em Disco Escherichia Coli Fosfomicina Hematologia Microbiologia Resistência Vitek® 2