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Multimodal ionic liquid-based chromatographic supports for an effective RNA purification

2023Journal article Open access
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dc.contributor.authorCarapito, Ana Rita Mugeiro
dc.contributor.authorBernardo, Sandra C.
dc.contributor.authorPereira, Matheus M.
dc.contributor.authorNeves, Márcia C.
dc.contributor.authorFreire, Mara G.
dc.contributor.authorSousa, Fani
dc.date.accessioned2023-05-02T10:43:45Z
dc.date.available2023-05-02T10:43:45Z
dc.date.issued2023
dc.description.abstractNucleic acids have been considered interesting molecules to be used as biopharmaceuticals for the treatment of various diseases, in gene therapy strategies. In particular, RNA arises as the most promising approach because it does not require access to the nucleus of cells to exert its function; however, it is quite challenging due to its labile nature. To increase the possibility of translating RNA-based technology to clinical protocols, the bio- manufacturing of RNAs has been intensively exploited in the last few years. However, the standard RNA puri- fication processes remain time-consuming and present limitations regarding recovery yield and purity. This work describes the functionalization of chromatographic silica-based supports with four ionic liquids (ILs) composed of functional moieties that can promote distinct interactions with nucleic acids. After an initial screening to evaluate the binding and elution behavior of nucleic acids in the IL-based supports, SSi[C3C3NH2Im]Cl has shown to be the most promising for further purification assays. This support was studied for the RNA purification from different samples (clarified or more complex) and has shown to be highly effective, for all the conditions studied. Generally, it is here presented a new method for RNA isolation in a single step, using an IL-based chromato- graphic support, able to eliminate the usage of hazardous compounds often included in standard RNA extraction protocols.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.doi10.1016/j.seppur.2023.123676pt_PT
dc.identifier.urihttp://hdl.handle.net/10400.6/13304
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.relationHealth Sciences Research Centre
dc.relationHealth Sciences Research Centre
dc.relationCICECO-Aveiro Institute of Materials
dc.relationCICECO-Aveiro Institute of Materials
dc.relationChemical Process Engineering and Forest Products Research Centre
dc.relationChemical Process Engineering and Forest Products Research Centre
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/pt_PT
dc.subjectNucleic acidspt_PT
dc.subjectIonic liquidpt_PT
dc.subjectIonic liquid-based supportpt_PT
dc.subjectMultimodal ligandpt_PT
dc.subjectPurificationpt_PT
dc.titleMultimodal ionic liquid-based chromatographic supports for an effective RNA purificationpt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleHealth Sciences Research Centre
oaire.awardTitleHealth Sciences Research Centre
oaire.awardTitleCICECO-Aveiro Institute of Materials
oaire.awardTitleCICECO-Aveiro Institute of Materials
oaire.awardTitleChemical Process Engineering and Forest Products Research Centre
oaire.awardTitleChemical Process Engineering and Forest Products Research Centre
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F00709%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDP%2F00709%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/9471 - RIDTI/PTDC%2FBII-BBF%2F29496%2F2017/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F50011%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDP%2F50011%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F00102%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDP%2F00102%2F2020/PT
oaire.citation.startPage123676pt_PT
oaire.citation.titleSeparation and Purification Technologypt_PT
oaire.citation.volume315pt_PT
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream9471 - RIDTI
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
person.familyNameCarapito
person.familyNameda Silva Bernardo
person.familyNameM. Pereira
person.familyNameNeves
person.familyNameFreire
person.familyNameSousa
person.givenNameAna Rita Mugeiro
person.givenNameSandra Cristina
person.givenNameMatheus
person.givenNameMárcia C.
person.givenNameMara
person.givenNameFani
person.identifier1342598
person.identifier.ciencia-id8E11-013D-1419
person.identifier.ciencia-id0616-8D07-D7B0
person.identifier.ciencia-id3C1F-F5A0-205C
person.identifier.ciencia-id411A-AD38-6EB1
person.identifier.ciencia-id6718-1145-692F
person.identifier.ciencia-id991D-2E13-A840
person.identifier.orcid0000-0003-2872-3609
person.identifier.orcid0000-0003-3524-6900
person.identifier.orcid0000-0001-5611-4080
person.identifier.orcid0000-0001-8895-0614
person.identifier.orcid0000-0001-9996-2194
person.identifier.ridGRF-0544-2022
person.identifier.ridB-8458-2008
person.identifier.ridA-2014-2017
person.identifier.scopus-author-id56096325200
person.identifier.scopus-author-id7103415227
person.identifier.scopus-author-id7102299235
person.identifier.scopus-author-id7005110268
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
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project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
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