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Androgen-responsive and nonresponsive prostate cancer cells present a distinct glycolytic metabolism profile

2012-11Journal article Restricted access
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dc.contributor.authorVaz, Cátia Alexandra Vicente
dc.contributor.authorAlves, Marco G.
dc.contributor.authorMarques, Ricardo
dc.contributor.authorMoreira, Paula I
dc.contributor.authorOliveira, P.F.
dc.contributor.authorBaptista, Cláudio
dc.contributor.authorSocorro, Sílvia
dc.date.accessioned2019-11-27T17:07:57Z
dc.date.available2019-11-27T17:07:57Z
dc.date.issued2012-11
dc.description.abstractProstate cancer (PCa) progresses from an early stage, confined to prostate, to a more aggressive metastasized cancer related with loss of androgen responsiveness. Although, it has been recognized that PCa cells have unique metabolic features, their glycolytic profile in androgen-dependent and androgen-independent stages of disease is much less known. Hence, the main purpose of this study was to compare glucose metabolism in androgen-responsive (LNCaP) and androgen-nonresponsive (PC3) PCa cells. Cell culture medium was collected and differences in glucose consumption and, lactate and alanine production were measured using Proton Nuclear Magnetic Resonance ((1)H NMR) spectra analysis. The mRNA and protein expression of glucose transporters (GLUT1 and GLUT3), phosphofructokinase 1 (PFK1), lactate dehydrogenase (LDH) and monocarboxylate transporter (MCT4) were determined by real-time PCR and Western Blot, respectively. The obtained results demonstrate that androgen-responsive (LNCaP) and androgen-nonresponsive (PC3) cells consumed similar amounts of glucose, whereas PC3 cells present higher lactate production. This increase in lactate production was concomitant with higher levels of MCT4 protein, increased LDH activity and higher lactate/alanine ratio, also suggesting increased levels of oxidative stress in PC3 cells. However, protein levels of LDH, associated with lactate metabolism, and GLUT3, involved in glucose uptake, were decreased in PC3 comparatively with LNCaP. Androgen-responsive and nonresponsive PCa cells present distinct glycolytic metabolism profiles, which suggest that targeting LDH and MCT4 metabolic pathways may be an important step for the development of new diagnostic and therapeutic strategies in the different stages of PCa.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.doi10.1016/j.biocel.2012.08.013pt_PT
dc.identifier.urihttp://hdl.handle.net/10400.6/7633
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.subjectPC3pt_PT
dc.subjectLNCaPpt_PT
dc.subjectAndrogenpt_PT
dc.subjectNMRpt_PT
dc.subjectMetabolomicspt_PT
dc.titleAndrogen-responsive and nonresponsive prostate cancer cells present a distinct glycolytic metabolism profilept_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage2084pt_PT
oaire.citation.issue11pt_PT
oaire.citation.startPage2077pt_PT
oaire.citation.titleThe International Journal of Biochemistry & Cell Biologypt_PT
oaire.citation.volume44pt_PT
person.familyNameVaz
person.familyNameAlves
person.familyNameMarques
person.familyNameMoreira
person.familyNameOliveira
person.familyNameMaia Baptista
person.familyNameSocorro
person.givenNameCátia
person.givenNameMarco G.
person.givenNameRicardo
person.givenNamePaula
person.givenNamePedro Fontes
person.givenNameCláudio Jorge
person.givenNameSílvia Cristina da Cruz Marques
person.identifier1232713
person.identifier4564
person.identifier2358493
person.identifierhttps://scholar.google.pt/citations?user=hFKOd_QAAAAJ&hl=pt-PT&oi=ao
person.identifierhttps://scholar.google.pt/citations?user=PA3L_h8AAAAJ&hl=pt-PT
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person.identifier.orcid0000-0002-7720-4234
person.identifier.orcid0000-0001-7635-783X
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person.identifier.scopus-author-id57202054274
person.identifier.scopus-author-id35358208900
person.identifier.scopus-author-id36198848500
person.identifier.scopus-author-id23097426600
person.identifier.scopus-author-id23097994600
rcaap.embargofctCopyright cedido à editora no momento da publicaçãopt_PT
rcaap.rightsclosedAccesspt_PT
rcaap.typearticlept_PT
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