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Arginine homopeptides for plasmid DNA purification using monolithic supports

2018Journal article Restricted access
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dc.contributor.authorCardoso, Sara
dc.contributor.authorSousa, Ângela
dc.contributor.authorQueiroz, João
dc.contributor.authorAzzoni, Adriano
dc.contributor.authorSousa, Fani
dc.date.accessioned2020-01-09T16:42:35Z
dc.date.available2020-01-09T16:42:35Z
dc.date.issued2018
dc.description.abstractPurification of plasmid DNA targeting therapeutic applications still presents many challenges, namely on supports and specific ligand development. Monolithic supports have emerged as interesting approaches for purifying pDNA due to its excellent mass transfer properties and higher binding capacity values. Moreover, arginine ligands were already described to establish specific and preferential interactions with pDNA. Additionally, some studies revealed the ability of arginine based cationic peptides to condense plasmid DNA, which increased lengthening can result in strongest interactions with higher binding capacities for chromatographic purposes of large molecules such as pDNA. In this work, arginine homopeptides were immobilized in monolithic supports and their performance was evaluated and compared with a single arginine monolithic column regarding supercoiled (sc) plasmid DNA purification. Specific interactions of arginine based peptides with several nucleic acids present in a clarified Escherichia coli lysate sample showed potential for the sc pDNA purification. Effectively, the immobilization of the arginine homopeptides became more functional compared with the single arginine amino acid, showing higher binding capacities, which was also reflected in the intensity of the interactions. The combination of structural versatilities of monoliths with the specificity of arginine peptides raised as a promising strategy for sc pDNA purification.pt_PT
dc.description.sponsorshipCNPq - Conselho Nacional de Desenvolvimento Científico e Tecnológico [grants 444412/2014-0, 307739/2015-5 and 140495/2016-9]; FAPESP - Fundação de Amparo à Pesquisa do Estado de São Paulo [grant 2013/23780-1].
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.doi10.1016/j.jchromb.2018.04.031pt_PT
dc.identifier.urihttp://hdl.handle.net/10400.6/8180
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherElsevierpt_PT
dc.relationPlatform for the biosynthesis and purification of HPV-16 E6/E7mutant DNA vaccine for cervical cancer treatment or prevention
dc.relation.publisherversionhttps://www.sciencedirect.com/science/article/pii/S1570023218301181?via%3Dihubpt_PT
dc.subjectArgininept_PT
dc.subjectChromatography - Affinitypt_PT
dc.subjectDNA - Superhelicalpt_PT
dc.subjectEscherichia colipt_PT
dc.subjectPeptidespt_PT
dc.subjectPlasmidspt_PT
dc.titleArginine homopeptides for plasmid DNA purification using monolithic supportspt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitlePlatform for the biosynthesis and purification of HPV-16 E6/E7mutant DNA vaccine for cervical cancer treatment or prevention
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/5876/UID%2FMulti%2F00709%2F2013/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT//SFRH%2FBPD%2F102716%2F2014/PT
oaire.citation.endPage157pt_PT
oaire.citation.startPage149pt_PT
oaire.citation.titleJournal of Chromatography Bpt_PT
oaire.citation.volume1087-1088pt_PT
oaire.fundingStream5876
person.familyNameSousa
person.familyNameQueiroz
person.familyNameAzzoni
person.familyNameSousa
person.givenNameÂngela
person.givenNameJoão
person.givenNameAdriano
person.givenNameFani
person.identifier817754
person.identifier.ciencia-idFB1F-08DE-23FE
person.identifier.ciencia-id931E-B66D-E341
person.identifier.ciencia-id991D-2E13-A840
person.identifier.orcid0000-0001-9155-7581
person.identifier.orcid0000-0002-3096-8325
person.identifier.orcid0000-0003-0696-4663
person.identifier.orcid0000-0001-9996-2194
person.identifier.ridL-3104-2014
person.identifier.ridA-2014-2017
person.identifier.scopus-author-id24330268800
person.identifier.scopus-author-id7003705645
person.identifier.scopus-author-id7003926883
person.identifier.scopus-author-id7005110268
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.embargofctCopyright cedido à editora no momento da publicaçãopt_PT
rcaap.rightsclosedAccesspt_PT
rcaap.typearticlept_PT
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