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iTRAQ Quantitative Proteomic Analysis of Vitreous from Patients with Retinal Detachment

2018Journal article Open access
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dc.contributor.authorSantos, F.M.
dc.contributor.authorGaspar, Leonor Isabel Mesquita
dc.contributor.authorCiordia, Sergio
dc.contributor.authorRocha, Ana
dc.contributor.authorSousa, João Paulo Castro De
dc.contributor.authorParadela, Alberto
dc.contributor.authorPassarinha, LA
dc.contributor.authorTomaz, C. T.
dc.date.accessioned2020-02-07T17:24:32Z
dc.date.available2020-02-07T17:24:32Z
dc.date.issued2018
dc.description.abstractRhegmatogenous retinal detachment (RRD) is a potentially blinding condition characterized by a physical separation between neurosensory retina and retinal pigment epithelium. Quantitative proteomics can help to understand the changes that occur at the cellular level during RRD, providing additional information about the molecular mechanisms underlying its pathogenesis. In the present study, iTRAQ labeling was combined with two-dimensional LC-ESI-MS/MS to find expression changes in the proteome of vitreous from patients with RRD when compared to control samples. A total of 150 proteins were found differentially expressed in the vitreous of patients with RRD, including 96 overexpressed and 54 underexpressed. Several overexpressed proteins, several such as glycolytic enzymes (fructose-bisphosphate aldolase A, gamma-enolase, and phosphoglycerate kinase 1), glucose transporters (GLUT-1), growth factors (metalloproteinase inhibitor 1), and serine protease inhibitors (plasminogen activator inhibitor 1) are regulated by HIF-1, which suggests that HIF-1 signaling pathway can be triggered in response to RRD. Also, the accumulation of photoreceptor proteins, including phosducin, rhodopsin, and s-arrestin, and vimentin in vitreous may indicate that photoreceptor degeneration occurs in RRD. Also, the accumulation of photoreceptor proteins, including phosducin, rhodopsin, and s-arrestin, and vimentin in vitreous may indicate that photoreceptor degeneration occurs in RRD. Nevertheless, the differentially expressed proteins found in this study suggest that different mechanisms are activated after RRD to promote the survival of retinal cells through complex cellular responses.pt_PT
dc.description.sponsorshipCENTRO-07-ST24-FEDER-002014; POCI-01-0145-FEDER-007491; CNB-CSIC proteomics lab is a member of ProteoRed, supported by PRB2-ISCIII grant [PT13/0001]; Novartis Farma-Produtos Farmacêuticos; PhD fellowship of Sciences Faculty financed by ICI and Santander.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.doi10.3390/ijms19041157pt_PT
dc.identifier.urihttp://hdl.handle.net/10400.6/9155
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherMDPIpt_PT
dc.relationVitreous humor biomarkers in Age-related Macular Degeneration
dc.subjectVitreous Proteomept_PT
dc.subjectRetinapt_PT
dc.subjectRetinal Detachmentpt_PT
dc.subjectRhodopsinpt_PT
dc.subjectiTRAQpt_PT
dc.subjectquantitative proteomicspt_PT
dc.titleiTRAQ Quantitative Proteomic Analysis of Vitreous from Patients with Retinal Detachmentpt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleVitreous humor biomarkers in Age-related Macular Degeneration
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/POR_CENTRO/SFRH%2FBD%2F112526%2F2015/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/5876/UID%2FMulti%2F00709%2F2013/PT
oaire.citation.issue4pt_PT
oaire.citation.startPage1157pt_PT
oaire.citation.titleInternational Journal of Molecular Sciencespt_PT
oaire.citation.volume19pt_PT
oaire.fundingStreamPOR_CENTRO
oaire.fundingStream5876
person.familyNameMilhano dos Santos
person.familyNameGaspar
person.familyNameCastro de Sousa
person.familyNameParadela
person.familyNamePaulino Passarinha
person.familyNameTomaz
person.givenNameFátima Raquel
person.givenNameLeonor Isabel Mesquita
person.givenNameJoão Paulo
person.givenNameAlberto
person.givenNameLuís António
person.givenNameCândida
person.identifierhttps://www.researchgate.net/profile/Luis_Passarinha
person.identifier2198714
person.identifier.ciencia-idD01A-2666-0E20
person.identifier.ciencia-id3216-14E9-65E0
person.identifier.ciencia-idD81B-D3DE-23D2
person.identifier.ciencia-id621B-74BF-F9A7
person.identifier.ciencia-idFE13-0C44-13D1
person.identifier.orcid0000-0002-4041-1504
person.identifier.orcid0000-0002-5106-1888
person.identifier.orcid0000-0002-0025-1841
person.identifier.orcid0000-0001-6837-7056
person.identifier.orcid0000-0001-6910-7576
person.identifier.orcid0000-0002-0927-2331
person.identifier.ridH-2113-2015
person.identifier.ridF-9367-2014
person.identifier.scopus-author-id56262249200
person.identifier.scopus-author-id6602585990
person.identifier.scopus-author-id12445439600
person.identifier.scopus-author-id7004546303
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
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