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Pichia pastoris: a recombinant microfactory for antibodies and human membrane proteins

2013-05Journal article Restricted access
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dc.contributor.authorGonçalves, A M
dc.contributor.authorPedro, Augusto
dc.contributor.authorMaia, C J
dc.contributor.authorSousa, Fani
dc.contributor.authorQueiroz, João
dc.contributor.authorPassarinha, L A
dc.date.accessioned2019-11-28T17:21:24Z
dc.date.available2019-11-28T17:21:24Z
dc.date.issued2013-05
dc.description.abstractDuring the last few decades, it has become evident that the compatibility of the yeast biochemical environment with the ability to process and translate the RNA transcript, along with its capacity to modify a translated protein, are relevant requirements for selecting this host cell for protein expression in several pharmaceutical and clinical applications. In particular, Pichia pastoris is used as an industrial host for recombinant protein and metabolite production, showing a powerful capacity to meet required biomolecular target production levels in high-throughput assays for functional genomics and drug screening. In addition, there is a great advantage to using P. pastoris for protein secretion, even at high molecular weights, since the recovery and purification steps are simplified owing to relatively low levels of endogenous proteins in the extracellular medium. Clearly, no single microexpression system can provide all of the desired properties for human protein production. Moreover, chemical and physical bioprocess parameters, including culture medium formulation, temperature, pH, agitation, aeration rates, induction, and feeding strategies, can highly influence product yield and quality. In order to benefit from the currently available wide range of biosynthesis strategies using P. pastoris, this mini review focuses on the developments and technological fermentation achievements, providing both a comparative and an overall integration analysis. The main aim is to highlight the relevance and versatility of the P. pastoris biosystem to the design of more cost-effective microfactories to meet the increasing demands for recombinant membrane proteins and clinical antibodies for several therapeutic applications.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.doi10.4014/jmb.1210.10063pt_PT
dc.identifier.urihttp://hdl.handle.net/10400.6/7642
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.relationStrategic Project - UI 709 - 2011-2012
dc.subjectPichia pastorispt_PT
dc.subjectHuman Membrane Proteinspt_PT
dc.subjectAntibodiespt_PT
dc.subjectBioprocess Designpt_PT
dc.titlePichia pastoris: a recombinant microfactory for antibodies and human membrane proteinspt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleStrategic Project - UI 709 - 2011-2012
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/5876-PPCDTI/EXPL%2FBBB-BQB%2F0960%2F2012/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/SFRH/SFRH%2FBD%2F81222%2F2011/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6820 - DCRRNI ID/PEst-C%2FSAU%2FUI0709%2F2011/PT
oaire.citation.endPage601pt_PT
oaire.citation.issue5pt_PT
oaire.citation.startPage587pt_PT
oaire.citation.titleInternational Journal of Microbiology and Biotechnologypt_PT
oaire.citation.volume23pt_PT
oaire.fundingStream5876-PPCDTI
oaire.fundingStreamSFRH
oaire.fundingStream6820 - DCRRNI ID
person.familyNameQuaresma Henriques Pedro
person.familyNameMaia Baptista
person.familyNameSousa
person.familyNameQueiroz
person.familyNamePaulino Passarinha
person.givenNameAugusto
person.givenNameCláudio Jorge
person.givenNameFani
person.givenNameJoão
person.givenNameLuís António
person.identifierhttps://scholar.google.com/citations?user=oer44UoAAAAJ&hl=pt-PT&oi=ao
person.identifierhttps://scholar.google.pt/citations?user=hFKOd_QAAAAJ&hl=pt-PT&oi=ao
person.identifierhttps://www.researchgate.net/profile/Luis_Passarinha
person.identifier.ciencia-id7111-B2BE-336D
person.identifier.ciencia-id5115-FCBD-A31F
person.identifier.ciencia-id991D-2E13-A840
person.identifier.ciencia-id931E-B66D-E341
person.identifier.ciencia-id621B-74BF-F9A7
person.identifier.orcid0000-0001-8194-6022
person.identifier.orcid0000-0002-5658-5445
person.identifier.orcid0000-0001-9996-2194
person.identifier.orcid0000-0002-3096-8325
person.identifier.orcid0000-0001-6910-7576
person.identifier.ridA-2014-2017
person.identifier.ridL-3104-2014
person.identifier.scopus-author-id54080426100
person.identifier.scopus-author-id23097426600
person.identifier.scopus-author-id7005110268
person.identifier.scopus-author-id7003705645
person.identifier.scopus-author-id12445439600
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.embargofctCopyright cedido à editora no momento da publicação.pt_PT
rcaap.rightsclosedAccesspt_PT
rcaap.typearticlept_PT
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