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Authors
Abstract(s)
O Vírus do Papiloma Humano (HPV) é uma das doenças sexualmente transmissíveis
mais comum em todo o mundo. Ambos os sexos estão envolvidos na cadeia
epidemiológica, podendo ser portadores assintomáticos, transmissores e vítimas deste
vírus. O vírus pode não ser eliminado pelo sistema imunológico, proporcionando o
desenvolvimento de certos cancros, como o cancro do colo do útero. Mais de 200
genótipos de HPV já foram sequenciados e caraterizados, encontrando-se divididos em
dois grupos: HPV de alto risco ou oncogénicos e HPV de baixo risco ou não oncogénicos.
Nas estirpes de HPV de alto risco destacam-se os tipos 16 e 18 sendo os mais cancerígenos
e com maior associação ao desenvolvimento de cancro do colo do útero.
As S-transferases da glutationa (GSTs) são uma superfamília de enzimas envolvidas na
manutenção da integridade celular, stress oxidativo e proteção contra danos no ADN,
reduzindo compostos através da conjugação com a glutationa (GSH) nas reações
metabólicas de fase II. A glutationa S-transferase mu 1 (GSTM1), pertencente a esta
família, é capaz de codificar uma enzima de fase II, responsável por destoxificar alguns
xenobióticos. Os polimorfismos do gene GSTM1 têm vindo a ser estudados devido ao seu
envolvimento em processos patológicos, como é o caso do cancro do colo do útero. O
polimorfismo nulo do GSTM1 tem como consequência a não codificação da enzima
GSTM1, o que compromete a destoxificação da célula, uma vez que reduz a metabolização
de substâncias tóxicas, favorecendo a carcinogénese. Assim, avaliar a possível relação
entre a deleção do GSTM1 e a infeção por HPV em lesões do colo do útero poderá ser um
elemento-chave para investigações futuras.
O objetivo principal deste trabalho, consistiu na determinação dos genótipos do
GSTM1 e das estirpes de HPV, em 37 mulheres com lesões no colo do útero, de modo a
avaliar uma possível relação entre a infeção por HPV e o genótipo do GSTM1. A
identificação do genótipo e das estirpes de HPV foi realizada a partir de tecido de lâminas
de tecido parafinado. A identificação dos genótipos do GSTM1 foi realizada por PCR em
tempo real, e as estirpes de HPV foram identificadas através do sistema Anyplex™ II HPV
HR Detection (Anyplex HR; Seegene, Seoul, Korea).
Os resultados foram analisados estatisticamente, tendo-se verificado que parece não
haver associação entre a deleção do GSTM1 e o desenvolvimento de lesões da cérvix
uterina causadas por HPV.
The Human Papilloma Virus (HPV) is one of the most common sexually transmitted diseases worldwide. Both sexes are involved in the epidemiological chain and may be asymptomatic carriers, transmitters, or victims of this virus. The immune system presents a crucial role in the elimination of this virus. However, if not eliminated, it may lead to the development of certain cancers, such as cervical cancer. More than 200 HPV genotypes have been sequenced and characterized being divided into 2 groups: high-risk or oncogenic HPV and low-risk or non-oncogenic HPV. High-risk HPV strains include types 16 and 18, which are the most carcinogenic and most associated with the development of cervical cancer. Glutathione S-transferases (GSTs) are a superfamily of enzymes that regulate cell integrity, oxidative stress, and protection against DNA damage by reducing compounds through conjugation with glutathione (GSH) in phase II metabolic reactions. Glutathione S-transferase mu 1 (GSTM1), belonging to this family, can encode a phase II enzyme responsible for detoxifying some xenobiotics. The gene GSTM1 polymorphism’s interest arises owing to their involvement in the pathological processes of cervical cancer. The null polymorphism of GSTM1 results in the non-coding of the GSTM1 enzyme, which compromises cell detoxification since it reduces the metabolisation of toxic substances, favouring carcinogenesis. Thus, assessing the possible relationship between GSTM1 deletion and HPV infection in cervical lesions may be a key element for future research. The study focuses were to determine the GSTM1 genotypes and HPV strains in 37 women with cervical lesions in order to evaluate a possible relationship between HPV infection and the GSTM1 genotype. Genotype and HPV strain identification was performed from paraffin-embedded tissue slides. GSTM1 genotype identification was performed by real-time PCR, and HPV strains were identified using the Anyplex™ II HPV HR Detection System (Anyplex HR; Seegene, Seoul, Korea). The results obtained showed that there seems to be no association between GSTM1 deletion, and the development of uterine cervix lesions caused by HPV.
The Human Papilloma Virus (HPV) is one of the most common sexually transmitted diseases worldwide. Both sexes are involved in the epidemiological chain and may be asymptomatic carriers, transmitters, or victims of this virus. The immune system presents a crucial role in the elimination of this virus. However, if not eliminated, it may lead to the development of certain cancers, such as cervical cancer. More than 200 HPV genotypes have been sequenced and characterized being divided into 2 groups: high-risk or oncogenic HPV and low-risk or non-oncogenic HPV. High-risk HPV strains include types 16 and 18, which are the most carcinogenic and most associated with the development of cervical cancer. Glutathione S-transferases (GSTs) are a superfamily of enzymes that regulate cell integrity, oxidative stress, and protection against DNA damage by reducing compounds through conjugation with glutathione (GSH) in phase II metabolic reactions. Glutathione S-transferase mu 1 (GSTM1), belonging to this family, can encode a phase II enzyme responsible for detoxifying some xenobiotics. The gene GSTM1 polymorphism’s interest arises owing to their involvement in the pathological processes of cervical cancer. The null polymorphism of GSTM1 results in the non-coding of the GSTM1 enzyme, which compromises cell detoxification since it reduces the metabolisation of toxic substances, favouring carcinogenesis. Thus, assessing the possible relationship between GSTM1 deletion and HPV infection in cervical lesions may be a key element for future research. The study focuses were to determine the GSTM1 genotypes and HPV strains in 37 women with cervical lesions in order to evaluate a possible relationship between HPV infection and the GSTM1 genotype. Genotype and HPV strain identification was performed from paraffin-embedded tissue slides. GSTM1 genotype identification was performed by real-time PCR, and HPV strains were identified using the Anyplex™ II HPV HR Detection System (Anyplex HR; Seegene, Seoul, Korea). The results obtained showed that there seems to be no association between GSTM1 deletion, and the development of uterine cervix lesions caused by HPV.
Description
Keywords
Cancro do Colo do Útero Gstm1 Hpv Polimorfismo