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Abstract(s)
A maior causa de consulta médica por parte das mulheres são as infecções vaginais e tem sido descrita uma procura maior de produtos com base em compostos naturais para o seu tratamento. A bacteriose vaginal é a infecção genital mais comum considerando-se que a presença de Gardnerella vaginalis parece ser a principal causa etilógica visto estar descrita em 95% dos casos. Esta bactéria é anaeróbia, Gram negativa mas com alguma variabilidade na coloração, dependendo da fase de crescimento em que se encontra e possui uma grande capacidade de adesão importante na patologia e diagnóstico de bacteriose vaginal pela formação de células epiteliais vaginais esfoliadas revestidas com G. vaginalis, conhecidas como ‘’clue cells’’. Diversos estudos têm como objecto a actividade antimicrobiana de compostos naturais devido ao aumento das resistências dos microrganismos aos antibióticos convencionais, criando assim uma terapêutica alternativa. Por outro lado a população lactobacilar parece desempenhar um papel protector, visto o seu papel probiótico, sendo a sua presença utilizada como um dos marcadores de flora vaginal normal. O uso de chá de Camellia sinensis em estudos científicos indica que é uma fonte natural e pouco dispendiosa de polifenóis, tais como os derivados de catequinas, que são conhecidos pelas suas propriedades antimicrobianas. Os benefícios do chá estão relacionados com a elevada quantidade de catequinas que constituem cerca de 30% do peso seco das folhas, sendo as principais a (-) epicatequina (EC), (-) epicatequina-3-galhato (ECG), (-) epigalhocatequina (EGC) e (-) epigalhocatequina-3-galhato (EGCG), sendo esta última catequina a que se encontra em maior quantidade no chá verde. Neste trabalho laboratorial avaliou-se o potencial antimicrobiano do extrato aquoso de chá verde dos Açores sobre estirpes de Gardnerella vaginalis, verificou-se a sobrevivência dos Lactobacillus spp. aquando da aplicação deste extrato aquoso e avaliou-se o mecanismo de acção do extrato aquoso de chá verde em Gardnerella vaginalis por citometria de fluxo. Os resultados sugerem que as estirpes de Lactobacillus spp. sobrevivem na presença do extrato aquoso de chá verde e as estirpes de Gardnerella vaginalis são susceptíveis ao mesmo extracto em concentração de CMI de 0,63mg/mL. Os resultados de citometria mostraram-se inconclusivos para este estudo. Deste modo, o extrato aquoso de chá verde dos Açores apresenta-se como um potencial agente terapêutico natural e de baixo custo, com potencial interesse nomeadamente na bacteriose vaginal.
Vaginal infections are the biggest cause of medical counsel by women and has been described an increased on demand for products based on natural compounds for their treatment. Bacterial vaginosis is the most frequent genital infection and the main etiological cause seems to be the presence of Gardnerella vaginalis, described in 95% of clinical cases. This is an anaerobic bacteria and Gram negative with coloration variable depending its growth stage and its adhesion capacity have an important role on diagnosis and pathology of bacterial vaginosis on formation of revested exfoliated vaginal epithelial cells with G. vaginalis, known as clue cells. Several studies are focused in antimicrobial activity of natural compounds due to increasing resistance of microorganisms to conventional antibiotics thus originate an alternative therapy. The lactobacilar population plays an importante role on protection due to its probiotic activity and its presence has been used as marken on normal vaginal flora. The use of Camellia sinensis tea on cientific studies presents that this tea is a natural and inexpensive source of polyphenols, as catechins derivates, known by its antimicrobial properties. Tea benefits are related with high amount of catechins, representing 30% of their dry weight of leaves, which the principals catechins are epicatechin (EC), (-) epicatechin-3-gallate (ECG), (-) epigallocatechin (EGC) and (-) epigallocatechin-3-gallate (EGCG), the last one is present in large amount in green tea. On this laboratorial study the antimicrobial potencial of aquous green tea from Azores extract has been rated on G. vaginalis strains and on Lactobacillus spp. survival and the action mechanism of green tea extract has been tested by flux cytometry. The results sugest strains of Lactobacillus spp. survive on green tea extract presence and strains of Gardnerella vaginalis present susceptibility at this extract which MIC value is 0,63mg/mL. The results of flux cytometry were inconclusive on this study. Thus, aquous green tea from Azores extract can be a potential natural terapeuthical agent and inexpensive against genital infections, mainly in bacterial vaginosis.
Vaginal infections are the biggest cause of medical counsel by women and has been described an increased on demand for products based on natural compounds for their treatment. Bacterial vaginosis is the most frequent genital infection and the main etiological cause seems to be the presence of Gardnerella vaginalis, described in 95% of clinical cases. This is an anaerobic bacteria and Gram negative with coloration variable depending its growth stage and its adhesion capacity have an important role on diagnosis and pathology of bacterial vaginosis on formation of revested exfoliated vaginal epithelial cells with G. vaginalis, known as clue cells. Several studies are focused in antimicrobial activity of natural compounds due to increasing resistance of microorganisms to conventional antibiotics thus originate an alternative therapy. The lactobacilar population plays an importante role on protection due to its probiotic activity and its presence has been used as marken on normal vaginal flora. The use of Camellia sinensis tea on cientific studies presents that this tea is a natural and inexpensive source of polyphenols, as catechins derivates, known by its antimicrobial properties. Tea benefits are related with high amount of catechins, representing 30% of their dry weight of leaves, which the principals catechins are epicatechin (EC), (-) epicatechin-3-gallate (ECG), (-) epigallocatechin (EGC) and (-) epigallocatechin-3-gallate (EGCG), the last one is present in large amount in green tea. On this laboratorial study the antimicrobial potencial of aquous green tea from Azores extract has been rated on G. vaginalis strains and on Lactobacillus spp. survival and the action mechanism of green tea extract has been tested by flux cytometry. The results sugest strains of Lactobacillus spp. survive on green tea extract presence and strains of Gardnerella vaginalis present susceptibility at this extract which MIC value is 0,63mg/mL. The results of flux cytometry were inconclusive on this study. Thus, aquous green tea from Azores extract can be a potential natural terapeuthical agent and inexpensive against genital infections, mainly in bacterial vaginosis.
Description
Keywords
Actividade Antimicrobiana Bacteriose Vaginal Camellia Sinensis Chá Verde dos Açores Gardnerella Vaginalis