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Solid-phase extraction for gas chromatographic mass spectrometric determination of the veterinary drug xylazine in human blood.

dc.contributor.authorGallardo, Eugenia
dc.contributor.authorBarroso, Mário
dc.contributor.authorMargalho, Cláudia
dc.contributor.authorDevesa, N.pt
dc.contributor.authorPimentel, J.pt
dc.contributor.authorVieira, Duarte Nuno
dc.date.accessioned2010-04-28T10:07:30Z
dc.date.available2010-04-28T10:07:30Z
dc.date.issued2007
dc.description.abstractThis paper presents a method for the determination of xylazine in whole blood using solid-phase extraction and gas chromatography–mass spectrometry. This technique required only 0.5 mL of sample, and protriptyline was used as internal standard (IS). Limits of detection and quantitation (LOQ) were 2 and 10 ng/mL, respectively. The method was found to be linear between the LOQ and 3.50 ìg/mL, with correlation coefficients higher than 0.9922. Precision (intra- and interday) and accuracy were in conformity with the criteria normally accepted in bioanalytical method validation. The analyte was stable in the matrix for at least 18 h at room temperature and for at least three freeze/thaw cycles. Mean recovery, calculated at three concentration levels, was 87%. To the best of our knowledge, this is the first time that solid-phase extraction is used as sample preparation technique for the determination of this compound in biological media. Because of its simplicity and speed when compared to other extraction techniques, the herein described method can be successfully applied in the diagnosis of intoxications by xylazine.pt
dc.identifier.urihttp://hdl.handle.net/10400.6/626
dc.languageeng
dc.titleSolid-phase extraction for gas chromatographic mass spectrometric determination of the veterinary drug xylazine in human blood.pt
dc.typejournal article
dspace.entity.typePublication
oaire.citation.issue37pt
oaire.citation.titleJournal of Analytical Toxicologypt
person.familyNameGallardo
person.familyNameBarroso
person.familyNameMargalho
person.familyNameVieira
person.givenNameEugenia
person.givenNameMário
person.givenNameCláudia
person.givenNameDuarte Nuno
person.identifier908303
person.identifier.ciencia-idF617-5CA5-EF61
person.identifier.ciencia-id5110-F476-ECCC
person.identifier.ciencia-idA211-521D-90CA
person.identifier.orcid0000-0002-1802-8998
person.identifier.orcid0000-0001-8848-2734
person.identifier.orcid0000-0003-1661-0367
person.identifier.orcid0000-0002-7366-6765
person.identifier.ridM-7410-2017
person.identifier.ridKII-2916-2024
person.identifier.scopus-author-id24721485300
person.identifier.scopus-author-id35329243100
person.identifier.scopus-author-id8655072100
person.identifier.scopus-author-id7005983374
rcaap.rightsopenAccess
rcaap.typearticlept
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relation.isAuthorOfPublication.latestForDiscoveryc1d97221-83ff-4292-9134-ce701c3adda7

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