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Advisor(s)
Abstract(s)
This paper presents a method for the determination of
xylazine in whole blood using solid-phase extraction and gas
chromatography–mass spectrometry. This technique required only
0.5 mL of sample, and protriptyline was used as internal standard
(IS). Limits of detection and quantitation (LOQ) were 2 and 10
ng/mL, respectively. The method was found to be linear between
the LOQ and 3.50 ìg/mL, with correlation coefficients higher than
0.9922. Precision (intra- and interday) and accuracy were in
conformity with the criteria normally accepted in bioanalytical
method validation. The analyte was stable in the matrix for at least
18 h at room temperature and for at least three freeze/thaw
cycles. Mean recovery, calculated at three concentration levels,
was 87%. To the best of our knowledge, this is the first time that
solid-phase extraction is used as sample preparation technique for
the determination of this compound in biological media. Because
of its simplicity and speed when compared to other extraction
techniques, the herein described method can be successfully
applied in the diagnosis of intoxications by xylazine.