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In Vivo High-Content Evaluation of Three-Dimensional Scaffolds Biocompatibility

2014-02-25Journal article Restricted access
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dc.contributor.authorOliveira, Mariana
dc.contributor.authorRibeiro, MP.
dc.contributor.authorMiguel, Sónia
dc.contributor.authorNeto, Ana
dc.contributor.authorCoutinho, Paula Isabel Teixeira Gonçalves
dc.contributor.authorCorreia, Ilídio Joaquim Sobreira
dc.contributor.authorMano, João F.
dc.date.accessioned2018-03-20T14:53:03Z
dc.date.available2018-03-20T14:53:03Z
dc.date.issued2014-02-25
dc.description.abstractWhile developing tissue engineering strategies, inflammatory response caused by biomaterials is an unavoidable aspect to be taken into consideration, as it may be an early limiting step of tissue regeneration approaches. We demonstrate the application of flat and flexible films exhibiting patterned high-contrast wettability regions as implantable platforms for the high-content in vivo study of inflammatory response caused by biomaterials. Screening biomaterials by using high-throughput platforms is a powerful method to detect hit spots with promising properties and to exclude uninteresting conditions for targeted applications. High-content analysis of biomaterials has been mostly restricted to in vitro tests where crucial information is lost, as in vivo environment is highly complex. Conventional biomaterials implantation requires the use of high numbers of animals, leading to ethical questions and costly experimentation. Inflammatory response of biomaterials has also been highly neglected in high-throughput studies. We designed an array of 36 combinations of biomaterials based on an initial library of four polysaccharides. Biomaterials were dispensed onto biomimetic superhydrophobic platforms with wettable regions and processed as freeze-dried three-dimensional scaffolds with a high control of the array configuration. These chips were afterward implanted subcutaneously in Wistar rats. Lymphocyte recruitment and activated macrophages were studied on-chip, by performing immunocytochemistry in the miniaturized biomaterials after 24 h and 7 days of implantation. Histological cuts of the surrounding tissue of the implants were also analyzed. Localized and independent inflammatory responses were detected. The integration of these data with control data proved that these chips are robust platforms for the rapid screening of early-stage in vivo biomaterials' response.pt_PT
dc.description.sponsorshipM.B.O. acknowledges Fundação para a Ciência e para a Tecnologia for the PhD grant SFRH/BD/71396/2010. The research leading to these results has received funding from the European Union’s Seventh Framework Programme (FP7/ 2007-2013) under grant agreement no. REGPOT-CT2012- 316331-POLARIS. The research was also funded by FEDER through the Competitive Factors Operation Program— COMPETE and by National funds through FCT—Fundação para a Ciência e a Tecnologia in the scope of the projects PTDC/CTM-BIO/1814/2012, Pest-C/SAU/UI0709/2011 and PEst-OE/EGE/UI4056/2011.
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationOliveira, M.B., Ribeiro, M.P., Miguel, S.P., Neto, A.I., Coutinho, P., Correia, I.J. e Mano, J.F. (2014) “In vivo high-content evaluation of three-dimensional scaffolds biocompatibility”, Tissue Engineering, Vol. 20(11): pp. 851-864pt_PT
dc.identifier.doi10.1089/ten.TEC.2013.0738pt_PT
dc.identifier.urihttp://hdl.handle.net/10400.6/4661
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherMary Ann Liebertpt_PT
dc.relationHIGH THROUGHPUT PHYSICOCHEMICAL CHARACTERIZATION AND CYTOCOMPATIBILITY ASSESSMENT OF CHITOSAN BASED SCAFFOLDS FOR TISSUE ENGINEERING
dc.relationMultilayered and hierarchically polymeric particles production for bone tissue engineering using a new biomimetic platform inspired on natural repellent substrates
dc.relationStrategic Project - UI 709 - 2011-2012
dc.relationStrategic Project - UI 4056 - 2011-2012
dc.relation.publisherversionhttps://www.liebertpub.com/doi/full/10.1089/ten.tec.2013.0738pt_PT
dc.subjectScaffoldspt_PT
dc.subjectBiocompatibilitypt_PT
dc.subjectThree Dimensionalpt_PT
dc.titleIn Vivo High-Content Evaluation of Three-Dimensional Scaffolds Biocompatibilitypt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleHIGH THROUGHPUT PHYSICOCHEMICAL CHARACTERIZATION AND CYTOCOMPATIBILITY ASSESSMENT OF CHITOSAN BASED SCAFFOLDS FOR TISSUE ENGINEERING
oaire.awardTitleMultilayered and hierarchically polymeric particles production for bone tissue engineering using a new biomimetic platform inspired on natural repellent substrates
oaire.awardTitleStrategic Project - UI 709 - 2011-2012
oaire.awardTitleStrategic Project - UI 4056 - 2011-2012
oaire.awardURIinfo:eu-repo/grantAgreement/FCT//SFRH%2FBD%2F71396%2F2010/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/5876-PPCDTI/PTDC%2FCTM-BIO%2F1814%2F2012/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6820 - DCRRNI ID/PEst-C%2FSAU%2FUI0709%2F2011/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/PEst-OE%2FEGE%2FUI4056%2F2011/PT
oaire.citation.endPage864pt_PT
oaire.citation.startPage851pt_PT
oaire.citation.titleTissue Engineeringpt_PT
oaire.citation.volume20pt_PT
oaire.fundingStream5876-PPCDTI
oaire.fundingStream6820 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
person.familyNameRibeiro
person.familyNamePereira Miguel
person.familyNameNeto
person.familyNameJoaquim Sobreira Correia
person.familyNameMano
person.givenNameMaximiano
person.givenNameSónia Alexandra
person.givenNameAna
person.givenNameIlídio
person.givenNameJoão
person.identifierGX0L2QAAAAJ
person.identifierUMbJ1KMAAAAJ
person.identifier.ciencia-idBD11-6497-FD37
person.identifier.ciencia-id1A13-ABC0-C32C
person.identifier.ciencia-idF610-7373-DC81
person.identifier.orcid0000-0001-6357-8115
person.identifier.orcid0000-0001-7274-0399
person.identifier.orcid0000-0001-5454-161X
person.identifier.orcid0000-0003-1613-9675
person.identifier.orcid0000-0002-2342-3765
person.identifier.ridO-1973-2017
person.identifier.ridA-4418-2009
person.identifier.scopus-author-id26658759200
person.identifier.scopus-author-id55638166400
person.identifier.scopus-author-id7003557499
person.identifier.scopus-author-id26643558900
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.embargofctCopyright cedido à editora no momento da publicaçãopt_PT
rcaap.rightsclosedAccesspt_PT
rcaap.typearticlept_PT
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