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Avaliação da atividade antifúngica de óleos essenciais de erva-príncipe combinado com clotrimazol no combate à candidíase vaginal
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Authors
Abstract(s)
O presente relatório foi escrito no âmbito da Unidade Curricular “Estágio”, com lugar no
último ano letivo do Mestrado Integrado em Ciências Farmacêuticas (MICF) da
Universidade da Beira Interior (UBI). O relatório é composto por três capítulos distintos,
sendo eles referentes à investigação conduzida no Centro de Investigação em Ciências da
Saúde da Universidade da Beira Interior (CICS-UBI), à experiência profissionalizante na
vertente de Farmácia Comunitária e à experiência profissionalizante na vertente de
Farmácia Hospitalar.
O primeiro capítulo é referente à investigação laboratorial conduzida no CICS-UBI,
denominada de “Avaliação da atividade antifúngica de óleos essenciais de erva-príncipe
combinada com clotrimazol no combate à candidíase vaginal”. Foram realizados vários
ensaios para determinar o índice de Concentração Inibitória Fracionada (FIC) dos óleos
essenciais de Cymbopogon citratus, Cymbopogon winterianus e Cymbopogon
flexuosus em associação com o clotrimazol, segundo o método de diluições seriadas, na
inibição do crescimento de Candida albicans. Com a combinação do clotrimazol com o
óleo essencial (OE) de Cymbopogon flexuosus obtiveram-se resultados próximos da
sinergia. Posteriormente, avaliou-se o impacto na formação de biofilmes da mesma
espécie, comparando o comportamento dos ativos em combinação e isoladamente, onde
as concentrações 0,0624 µg/mL de clotrimazol e a 0,0313% (V/V) de OE, a combinação
(mix) revelou-se maioritariamente mais eficaz no combate à formação de biofilmes,
representando uma estratégia eficaz para superar as resistências aos antifúngicos. No
entanto, às concentrações dez vezes superiores dos ativos os resultados foram menos
promissores.
O segundo capítulo descreve a experiência profissionalizante em Farmácia Hospitalar,
concretamente no Hospital São Teotónio do Centro Hospitalar Tondela-Viseu (CHTV).
O estágio ocorreu de 4 de março de 2024 a 26 de abril de 2024. Neste relatório estão
descritas as atividades abordadas durante 2 meses de estágio, onde tive oportunidade de
passar por todos os setores dos Serviços Farmacêuticos do CHTV, desde a receção e
armazenamento à dispensa de medicação. O percurso pelo hospital foi fundamental para
o meu desenvolvimento enquanto Farmacêutica, onde complementei todos os
conhecimentos adquiridos ao longo do curso na vertente hospitalar O terceiro capítulo descreve a experiência profissionalizante em Farmácia Comunitária,
concretamente na Farmácia Grão Vasco, em Viseu. O estágio ocorreu de 29 de abril de
2024 a 19 de julho de 2024. No presente relatório está descrito o meu percurso pela
vertente de Farmácia Comunitária, com relato de algumas situações reais de dispensa ao
público. Tive oportunidade de acompanhar todos os setores da Farmácia Comunitária e
rematar este percurso académico com uma visão holística do que é ser Farmacêutica.
This report was written as part of the "Internship" course unit during the final academic year of the Integrated Master's in Pharmaceutical Sciences (MICF) at the University of Beira Interior (UBI). It is composed of three distinct chapters, which address the research conducted at the Health Sciences Research Center of the University of Beira Interior (CICS-UBI), professional experience in Community Pharmacy, and professional experience in Hospital Pharmacy. The first chapter focuses on laboratory research conducted at CICS-UBI under the title "Evaluation of the antifungal activity of lemongrass essential oils combined with clotrimazole in combating vaginal candidiasis." Several assays were carried out to determine the Fractional Inhibitory Concentration Index (FIC) of the essential oils of Cymbopogon citratus, Cymbopogon winterianus and Cymbopogon flexuosus in combination with clotrimazole, using the serial dilution method, in the inhibition of Candida albicans growth. The combination of clotrimazole with the essential oil (EO) of Cymbopogon flexuosus yielded results close to synergy. Subsequently, the impact on biofilm formation of the same species was evaluated, comparing the behavior of the agents in combination and individually. At concentrations of 0.0624 µg/mL of clotrimazole and 0.0313% (V/V) of EO, the combination proved to be more effective in combating biofilm formation, representing an effective strategy to overcome antifungal resistance. However, at tenfold higher concentrations of the active ingredients, the results were less promising. The second chapter describes the professional experience gained during an internship in Hospital Pharmacy at São Teotónio Hospital of the Tondela-Viseu Hospital Center (CHTV). The internship took place from March 4, 2024, to April 26, 2024. This chapter outlines the activities undertaken during the two-month internship, where I had the opportunity to work in all sectors of the CHTV’s Pharmaceutical Services, from medication reception and storage to dispensing. This hospital experience was fundamental in my development as a pharmacist, complementing the knowledge acquired throughout the course with a focus on the hospital setting. The third chapter details the professional experience gained during the internship in Community Pharmacy at Grão Vasco Pharmacy in Viseu. This internship took place from April 29, 2024, to July 19, 2024. This chapter describes my journey in Community Pharmacy, including real-life situations of dispensing medication to the public. I had the opportunity to work in all sectors of Community Pharmacy, concluding my academic path with a holistic understanding of what it means to be a pharmacist.
This report was written as part of the "Internship" course unit during the final academic year of the Integrated Master's in Pharmaceutical Sciences (MICF) at the University of Beira Interior (UBI). It is composed of three distinct chapters, which address the research conducted at the Health Sciences Research Center of the University of Beira Interior (CICS-UBI), professional experience in Community Pharmacy, and professional experience in Hospital Pharmacy. The first chapter focuses on laboratory research conducted at CICS-UBI under the title "Evaluation of the antifungal activity of lemongrass essential oils combined with clotrimazole in combating vaginal candidiasis." Several assays were carried out to determine the Fractional Inhibitory Concentration Index (FIC) of the essential oils of Cymbopogon citratus, Cymbopogon winterianus and Cymbopogon flexuosus in combination with clotrimazole, using the serial dilution method, in the inhibition of Candida albicans growth. The combination of clotrimazole with the essential oil (EO) of Cymbopogon flexuosus yielded results close to synergy. Subsequently, the impact on biofilm formation of the same species was evaluated, comparing the behavior of the agents in combination and individually. At concentrations of 0.0624 µg/mL of clotrimazole and 0.0313% (V/V) of EO, the combination proved to be more effective in combating biofilm formation, representing an effective strategy to overcome antifungal resistance. However, at tenfold higher concentrations of the active ingredients, the results were less promising. The second chapter describes the professional experience gained during an internship in Hospital Pharmacy at São Teotónio Hospital of the Tondela-Viseu Hospital Center (CHTV). The internship took place from March 4, 2024, to April 26, 2024. This chapter outlines the activities undertaken during the two-month internship, where I had the opportunity to work in all sectors of the CHTV’s Pharmaceutical Services, from medication reception and storage to dispensing. This hospital experience was fundamental in my development as a pharmacist, complementing the knowledge acquired throughout the course with a focus on the hospital setting. The third chapter details the professional experience gained during the internship in Community Pharmacy at Grão Vasco Pharmacy in Viseu. This internship took place from April 29, 2024, to July 19, 2024. This chapter describes my journey in Community Pharmacy, including real-life situations of dispensing medication to the public. I had the opportunity to work in all sectors of Community Pharmacy, concluding my academic path with a holistic understanding of what it means to be a pharmacist.
Description
Keywords
Farmácia Comunitária Biofilme Candida Albicans Candidíase Vaginal Clotrimazol Cymbopogon Flexuosus Farmácia Hospitalar Óleo Essencial