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Circulating endothelial cells in patients with venous thromboembolism and myeloproliferative neoplasms

2013Journal article Open access
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dc.contributor.authorTorres, Cláudia
dc.contributor.authorFonseca, Ana Mafalda
dc.contributor.authorLeander, Magdalena
dc.contributor.authorMatos, Rui
dc.contributor.authorMorais, Sara
dc.contributor.authorCampos, Manuel
dc.contributor.authorLima, Margarida
dc.date.accessioned2020-02-28T15:24:30Z
dc.date.available2020-02-28T15:24:30Z
dc.date.issued2013
dc.description.abstractBackground: Circulating endothelial cells (CEC) may be a biomarker of vascular injury and pro-thrombotic tendency, while circulating endothelial progenitor cells (CEP) may be an indicator for angiogenesis and vascular remodelling. However, there is not a universally accepted standardized protocol to identify and quantify these cells and its clinical relevancy remains to be established. Objectives: To quantify CEC and CEP in patients with venous thromboembolism (VTE) and with myeloproliferative neoplasms (MPN), to characterize the CEC for the expression of activation (CD54, CD62E) and procoagulant (CD142) markers and to investigate whether they correlate with other clinical and laboratory data. Patients and Methods: Sixteen patients with VTE, 17 patients with MPN and 20 healthy individuals were studied. The CEC and CEP were quantified and characterized in the blood using flow cytometry, and the demographic, clinical and laboratory data were obtained from hospital records. Results: We found the CEC counts were higher in both patient groups as compared to controls, whereas increased numbers of CEP were found only in patients with MPN. In addition, all disease groups had higher numbers of CD62E+ CEC as compared to controls, whereas only patients with VTE had increased numbers of CD142+ and CD54+ CEC. Moreover, the numbers of total and CD62+ CEC correlated positively with the white blood cells (WBC) counts in both groups of patients, while the numbers of CEP correlated positively with the WBC counts only in patients with MPN. In addition, in patients with VTE a positive correlation was found between the numbers of CD54+ CEC and the antithrombin levels, as well as between the CD142+ CEC counts and the number of thrombotic events. Conclusions: Our study suggests that CEC counts may reveal endothelial injury in patients with VTE and MPN and that CEC may express different activation-related phenotypes depending on the disease status.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.doi10.1371/journal.pone.0081574pt_PT
dc.identifier.urihttp://hdl.handle.net/10400.6/9629
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/pt_PT
dc.subjectBiomarkerspt_PT
dc.subjectCell Countpt_PT
dc.subjectEndothelial Cellspt_PT
dc.subjectHumanspt_PT
dc.subjectPolycythemia Verapt_PT
dc.subjectRisk Factorspt_PT
dc.subjectStem Cellspt_PT
dc.subjectThrombocythemia Essentialpt_PT
dc.subjectVenous Thromboembolismpt_PT
dc.titleCirculating endothelial cells in patients with venous thromboembolism and myeloproliferative neoplasmspt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.issue12pt_PT
oaire.citation.startPagee81574pt_PT
oaire.citation.titlePLoS ONEpt_PT
oaire.citation.volume8pt_PT
person.familyNameLoureiro Fonseca
person.familyNameLeander
person.familyNameMorais
person.familyNameLima
person.givenNameAna Mafalda
person.givenNameMagdalena
person.givenNameSara
person.givenNameMargarida
person.identifierH-3836-2012
person.identifier.ciencia-idA61D-657A-6DD0
person.identifier.ciencia-id8410-F6C9-183F
person.identifier.orcid0000-0003-1118-5525
person.identifier.orcid0000-0002-3050-2672
person.identifier.orcid0000-0003-4266-4457
person.identifier.orcid0000-0001-9702-5260
person.identifier.ridS-7466-2016
person.identifier.scopus-author-id7007053742
person.identifier.scopus-author-id7202143317
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublication34cfbd2d-5537-4aba-8552-3e2e4d48b473
relation.isAuthorOfPublication41610d76-64f4-49bc-ad43-95ada8d9b1d3
relation.isAuthorOfPublication01b687ec-5a1d-4c83-806a-c7e28d61f3c9
relation.isAuthorOfPublication254a853c-4b12-4da5-aef9-8479bb4a2ba0
relation.isAuthorOfPublication.latestForDiscovery01b687ec-5a1d-4c83-806a-c7e28d61f3c9

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