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Highly selective capture of minicircle DNA biopharmaceuticals by a novel zinc-histidine peptide conjugate

2016-10-31Journal article Restricted access
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dc.contributor.authorGaspar, Vítor Manuel Abreu
dc.contributor.authorCruz, Carla Patrícia Alves Freire Madeira
dc.contributor.authorQueiroz, João
dc.contributor.authorPichon, Chantal
dc.contributor.authorCorreia, Ilídio
dc.contributor.authorSousa, Fani
dc.date.accessioned2018-03-22T08:45:52Z
dc.date.available2018-03-22T08:45:52Z
dc.date.issued2016-10-31
dc.description.abstractThe use of minicircle DNA (mcDNA) biomolecules as a pharmaceutical product holds remarkable potential due to their improved therapeutic efficacy in comparison with standard non-viral gene expression vectors. However, mcDNA translation into clinical application is still highly restricted due to the lack of robust technologies for minicircles detection and purification. In this study, the potential of a zinc-binding histidine-based peptide to function as a novel ligand for mcDNA recovery was investigated by using high-throughput surface plasmon resonance (SPR) analysis. The histidine-based peptide successfully bound zinc cationic ions and had affinity towards mcDNA biomolecules as confirmed by the dynamic binding responses obtained in SPR experiments. Notably, the obtained results indicate that not only zinc-peptide ligands are able to bind mcDNA with very high affinity (KD = 4.21 × 10−10 M), but also that this interaction is mostly dependent on buffer type. In general, the findings indicated that Zn2+ bound peptide has high affinity to mcDNA in low ionic strength buffers, whereas with high salt buffers no binding is detected. Overall, the novel zinc-binding peptide has shown to have suitable properties for mcDNA binding and recovery under experimental conditions that assure genetic material stability. More importantly, the straightforward approach of employing simple biomimetic ligands for mcDNA capture will contribute for development of new technologies to purify DNA biopharmaceuticals.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationGaspar, V. M., Cruz, C., Queiroz, J. A., Pichon, C., Correia, I. J., e Sousa, F. (2017) “Highly selective capture of minicircle DNA biopharmaceuticals by a novel zinc-histidine peptide conjugate.” Separation and Purification Technology, Vol.174, pp.417-424pt_PT
dc.identifier.doi10.1016/j.seppur.2016.10.054pt_PT
dc.identifier.urihttp://hdl.handle.net/10400.6/4693
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherElsevierpt_PT
dc.relationStrategic Project - UI 709 - 2011-2012
dc.relationG-quadruplex binders for cancer therapy
dc.relationPurification, detection and stabilization of G-quadruplex for cancer therapy
dc.relation.publisherversionhttps://www.sciencedirect.com/science/article/pii/S1383586616313661#f0035pt_PT
dc.subjectMinicircle DNApt_PT
dc.subjectAffinity interactionspt_PT
dc.subjectBiopharmaceuticalspt_PT
dc.subjectBiomimetic peptidept_PT
dc.titleHighly selective capture of minicircle DNA biopharmaceuticals by a novel zinc-histidine peptide conjugatept_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleStrategic Project - UI 709 - 2011-2012
oaire.awardTitleG-quadruplex binders for cancer therapy
oaire.awardTitlePurification, detection and stabilization of G-quadruplex for cancer therapy
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6820 - DCRRNI ID/PEst-C%2FSAU%2FUI0709%2F2011/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/5876-PPCDTI/EXPL%2FQEQ-MED%2F1068%2F2013/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT//SFRH%2FBPD%2F100015%2F2014/PT
oaire.citation.endPage424pt_PT
oaire.citation.startPage417pt_PT
oaire.citation.titleSeparation and Purification Technologypt_PT
oaire.citation.volume174pt_PT
oaire.fundingStream6820 - DCRRNI ID
oaire.fundingStream5876-PPCDTI
person.familyNameGaspar
person.familyNameQueiroz
person.familyNameJoaquim Sobreira Correia
person.familyNameSousa
person.givenNameVítor
person.givenNameJoão
person.givenNameIlídio
person.givenNameFani
person.identifierUMbJ1KMAAAAJ
person.identifier.ciencia-id6F16-3640-73E3
person.identifier.ciencia-id931E-B66D-E341
person.identifier.ciencia-idF610-7373-DC81
person.identifier.ciencia-id991D-2E13-A840
person.identifier.orcid0000-0002-0372-2493
person.identifier.orcid0000-0002-3096-8325
person.identifier.orcid0000-0003-1613-9675
person.identifier.orcid0000-0001-9996-2194
person.identifier.ridB-1602-2017
person.identifier.ridL-3104-2014
person.identifier.ridA-2014-2017
person.identifier.scopus-author-id36968590900
person.identifier.scopus-author-id7003705645
person.identifier.scopus-author-id7003557499
person.identifier.scopus-author-id7005110268
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.embargofctCopyright cedido à editora no momento da publicaçãopt_PT
rcaap.rightsclosedAccesspt_PT
rcaap.typearticlept_PT
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