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Biofunctionalization of cellulosic fibres with l-cysteine: Assessment of antibacterial properties and mechanism of action against Staphylococcus aureus and Klebsiella pneumoniae

2013Journal article Open access
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dc.contributor.authorCaldeira, Estela
dc.contributor.authorPiskin, Erhan
dc.contributor.authorGranadeiro, Luiza Breitenfeld
dc.contributor.authorSilva, Filomena
dc.contributor.authorGouveia, Isabel C.
dc.date.accessioned2022-01-17T10:20:25Z
dc.date.available2022-01-17T10:20:25Z
dc.date.issued2013
dc.description.abstractThe main purpose of this work is to obtain a cotton-based textile material functionalized with l-cysteine (l-cys) to achieve an antimicrobial effect with potential application in biomedical, geriatric or pediatric textiles. The binding capacity of l-cys to cotton fibres was assessed through different functionalization strategiesā€”surface activation and exhaustion processes. A subsequent analysis of the possible antibacterial action against Staphylococcus aureus and Klebsiella pneumoniae was performed according with the Japanese International standard (JISL, 2008). To determine the mechanism of action of l-cys on the selected strains, flow cytometry was used. The results revealed that the exhaustion process was performed with success to confer bioactivity to the treated fabric, as assessed by an effective antibacterial effect against both Gram-positive and Gram-negative bacteria, and successfully linkage of l-cys was observed via FTIR with a durable effect demonstrated after the washing tests (fastness to washing). It was also observed that l-cys exerts a bacteriostatic effect against both bacterial strains, since there were alterations in the metabolic activity ofthe microorganisms after the application of the bioactive textile which was shown by the CTC (cyanoditolyl tetrazolium chloride) staining used in flow cytometry. This study shows a new and successful biotechnological process to develop antibacterial textiles through the functionalization of cotton fibres with l-cys which presents a broad range of applications in healthcare, since l-cys is a natural antibacterial compound, non-toxic and affects pathogenic bacteria related to hospital infections.pt_PT
dc.description.sponsorshipThe authors would like to thank FundacĀø Ć£o para a CiĆŖncia e Tecnologia (FCT) for the funding granted concerning the projectā€”PTDC/EBB-BIO/113671/2009 (FCOMP-01-0124-FEDER014752) Skin2Tex. We also would like to thank Fundo Europeu de Desenvolvimento Regional (FEDER) through COMPETEā€“Programa Operacional Factores de Competitividade (POFC) for the cofunding.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.doi10.1016/j.jbiotec.2013.10.021pt_PT
dc.identifier.urihttp://hdl.handle.net/10400.6/11838
dc.language.isoengpt_PT
dc.relationSkin2Tex Old peptides with new faces: A new strategy to develop non-toxic antimicrobial textiles for healthcare applications.
dc.subjectAntimicrobial-textilespt_PT
dc.subjectCellulosept_PT
dc.subjectAntimicrobial agentspt_PT
dc.subjectStaphylococcus aureuspt_PT
dc.subjectKlebsiella pneumoniaept_PT
dc.subjectFlow cytometrypt_PT
dc.titleBiofunctionalization of cellulosic fibres with l-cysteine: Assessment of antibacterial properties and mechanism of action against Staphylococcus aureus and Klebsiella pneumoniaept_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleSkin2Tex Old peptides with new faces: A new strategy to develop non-toxic antimicrobial textiles for healthcare applications.
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/5876-PPCDTI/PTDC%2FEBB-BIO%2F113671%2F2009/PT
oaire.citation.endPage435pt_PT
oaire.citation.issue4pt_PT
oaire.citation.startPage426pt_PT
oaire.citation.titleJournal of Biotechnologypt_PT
oaire.citation.volume168pt_PT
oaire.fundingStream5876-PPCDTI
person.familyNameGranadeiro
person.familyNameSilva
person.familyNameGouveia
person.givenNameLuiza Breitenfeld
person.givenNameFilomena
person.givenNameIsabel Cristina
person.identifier274116
person.identifier1396147
person.identifier626131
person.identifier.ciencia-idE71F-B2E5-D657
person.identifier.ciencia-idF91E-2365-F208
person.identifier.ciencia-id5711-86D0-A54B
person.identifier.orcid0000-0001-8550-1645
person.identifier.orcid0000-0003-2931-4583
person.identifier.orcid0000-0003-3290-9529
person.identifier.ridP-1852-2015
person.identifier.ridA-3091-2013
person.identifier.ridK-1651-2014
person.identifier.scopus-author-id34467456600
person.identifier.scopus-author-id35723100200
person.identifier.scopus-author-id24328741500
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundaĆ§Ć£o para a CiĆŖncia e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
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relation.isAuthorOfPublication634daea1-f9c3-4554-bdcc-0e65a3967251
relation.isAuthorOfPublicationa2024ec6-6edb-47e5-b8a0-594066a5036c
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