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Preparation of well-defined brush-like block copolymers for gene delivery applications under biorelevant reaction conditions

2018Journal article Restricted access
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dc.contributor.authorGóis, Joana R.
dc.contributor.authorReis, Fábio
dc.contributor.authorAlmeida, Ana Margarida
dc.contributor.authorPereira, Patrícia
dc.contributor.authorSousa, Fani
dc.contributor.authorSerra, Armenio
dc.contributor.authorCoelho, Jorge
dc.date.accessioned2020-01-09T16:48:33Z
dc.date.available2020-01-09T16:48:33Z
dc.date.issued2018
dc.description.abstractWell-defined oligo(ethylene glycol) methyl ether methacrylate (OEOMA) based block copolymers with cationic segments composed by N,N-(dimethylamino) ethyl methacrylate (DMAEMA) and/or 2-(diisopropylamino) ethyl methacrylate (DPA) were developed under biorelevant reaction conditions. These brush-type copolymers were synthesized through supplemental activator and reducing agent (SARA) atom transfer radical polymerization (ATRP) using sodium dithionite as SARA agent. The synthesis was carried out using an eco-friendly solvent mixture, very low copper catalyst concentration, and mild reaction conditions. The structure of the block copolymers was characterized by size exclusion chromatography (SEC) analysis and 1H nuclear magnetic resonance (NMR) spectroscopy. The pH-dependent protonation of these copolymers enables the efficient complexation with plasmid DNA (pDNA), yielding polyplexes with sizes ranging from 200 up to 700 nm, depending on the molecular weight of the copolymers, composition and concentration used. Agarose gel electrophoresis confirmed the successful pDNA encapsulation. No cytotoxicity effect was observed, even for N/P ratios higher than 50, for human fibroblasts and cervical cancer cell lines cells. The in vitro cellular uptake experiments demonstrated that the pDNA-loaded block copolymers were efficiently delivered into nucleus of cervical cancer cells. The polymerization approach, the unique structure of the block copolymers and the efficient DNA encapsulation presented can open new avenues for development of efficient tailor made gene delivery systems under biorelevant conditions.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.doi10.1016/j.colsurfb.2018.05.004pt_PT
dc.identifier.urihttp://hdl.handle.net/10400.6/8181
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherElsevierpt_PT
dc.relationLight activated permanent antimicrobial surfaces
dc.relation.publisherversionhttps://www.sciencedirect.com/science/article/pii/S0927776518302807?via%3Dihubpt_PT
dc.subjectCell Linept_PT
dc.subjectCell Nucleuspt_PT
dc.subjectCell Survivalpt_PT
dc.subjectDNApt_PT
dc.subjectElectrophoresis - Agar Gelpt_PT
dc.subjectEthylene Glycolspt_PT
dc.subjectHumanspt_PT
dc.subjectMethylmethacrylatept_PT
dc.subjectParticle Sizept_PT
dc.subjectPlasmidspt_PT
dc.subjectPolymerizationpt_PT
dc.subjectPolymerspt_PT
dc.subjectSurface Propertiespt_PT
dc.subjectGene Transfer Techniquespt_PT
dc.titlePreparation of well-defined brush-like block copolymers for gene delivery applications under biorelevant reaction conditionspt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleLight activated permanent antimicrobial surfaces
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/9471 - RIDTI/PTDC%2FCTM-POL%2F6138%2F2014/PT
oaire.citation.endPage117pt_PT
oaire.citation.startPage107pt_PT
oaire.citation.titleColloids and Surfaces B: Biointerfacespt_PT
oaire.citation.volume169pt_PT
oaire.fundingStream9471 - RIDTI
person.familyNameGóis
person.familyNameAlmeida
person.familyNamePereira
person.familyNameSousa
person.familyNameSerra
person.familyNameJordão Coelho
person.givenNameJoana Rita
person.givenNameAna Margarida
person.givenNamePatrícia
person.givenNameFani
person.givenNameArmenio
person.givenNameJorge Fernando
person.identifier1073396
person.identifierhttps://scholar.google.com/citations?user=fiYocSIAAAAJ&hl=en
person.identifier.ciencia-idCC15-A4B6-AA64
person.identifier.ciencia-id4715-067B-4FD0
person.identifier.ciencia-id991D-2E13-A840
person.identifier.ciencia-id5711-73B5-EAAE
person.identifier.ciencia-id6C1B-87C8-2C4A
person.identifier.orcid0000-0003-1828-0728
person.identifier.orcid0000-0002-9313-6649
person.identifier.orcid0000-0002-5665-5271
person.identifier.orcid0000-0001-9996-2194
person.identifier.orcid0000-0001-8664-2757
person.identifier.ridA-2828-2014
person.identifier.ridA-2014-2017
person.identifier.ridC-9698-2014
person.identifier.scopus-author-id35733669300
person.identifier.scopus-author-id56111844900
person.identifier.scopus-author-id7005110268
person.identifier.scopus-author-id7201849354
person.identifier.scopus-author-id7101841069
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.embargofctCopyright cedido à editora no momento da publicaçãopt_PT
rcaap.rightsclosedAccesspt_PT
rcaap.typearticlept_PT
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