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Authors
Abstract(s)
A prevalência de infeções causadas por fungos emergentes é motivo de grande preocupação e debate na comunidade científica. A epidemiologia do género Candida está bem documentada. No entanto, a epidemiologia do género Rhodotorula subsiste de forma oblíqua. O presente estudo visa contribuir para a identificação destes agentes patogénicos bem como para a determinação do perfil de suscetibilidade in vitro a compostos azólicos. Adicionalmente, a estabilidade do genoma e as características fenotípicas dos isolados foram testadas. Quinze isolados da vulva de 12 mulheres sintomáticas foram identificados através do sistema Vitek®. A suscetibilidade ao fluconazol foi determinada de acordo com o método de microdiluição CLSI. Foram avaliadas as principais características macro morfológicas e o perfil genotípico foi determinado pela técnica de RAPD-PCR. Como principais resultados, observamos variações na identificação Vitek® versus métodos moleculares. Os isolados de Rhodotorula apresentaram resistência a fluconazol. Possíveis alterações nos padrões de amplificação por RAPD-PCR podem estar intrinsecamente relacionadas à capacidade adaptativa das leveduras. No entanto, mais investigações são necessárias neste campo. Com base nos dados obtidos é possível concluir sobre a necessidade de novas abordagens diagnósticas, para introdução de terapia específica, que permita a adoção de medidas de prevenção e controle destas infeções.
The prevalence of infections caused by emerging fungi is a matter of great concern and debate in the scientific community. The epidemiology of the Candida genus is well documented. However, the epidemiology of the genus Rhodotorula remains askew. The present study aims to contribute to the identification of these pathogens as well as the determination of the in vitro susceptibility profile to azole compounds. Furthermore, the stability of the genome and the phenotypic characteristics of the isolates were tested. Fifteen isolates from the vulva of 12 symptomatic women, were identified using the Vitek® system. Susceptibility to fluconazole was determined according to the CLSI microdilution method. The main macro-morphological characteristics were evaluated, and the genotypic profile was determined by the RAPD-PCR technique. As main results, we observed variations in Vitek® identification versus molecular methods. Rhodotorula isolates were resistant to fluconazole. Possible alterations in the amplification patterns by RAPD-PCR may be intrinsically related to the adaptive capacity of yeasts. However, further investigations are needed in this field. Based on the data obtained, it is possible to conclude the need for new diagnostic approaches, for the introduction of specific therapy, which allows the adoption of prevention and control measures for these infections.
The prevalence of infections caused by emerging fungi is a matter of great concern and debate in the scientific community. The epidemiology of the Candida genus is well documented. However, the epidemiology of the genus Rhodotorula remains askew. The present study aims to contribute to the identification of these pathogens as well as the determination of the in vitro susceptibility profile to azole compounds. Furthermore, the stability of the genome and the phenotypic characteristics of the isolates were tested. Fifteen isolates from the vulva of 12 symptomatic women, were identified using the Vitek® system. Susceptibility to fluconazole was determined according to the CLSI microdilution method. The main macro-morphological characteristics were evaluated, and the genotypic profile was determined by the RAPD-PCR technique. As main results, we observed variations in Vitek® identification versus molecular methods. Rhodotorula isolates were resistant to fluconazole. Possible alterations in the amplification patterns by RAPD-PCR may be intrinsically related to the adaptive capacity of yeasts. However, further investigations are needed in this field. Based on the data obtained, it is possible to conclude the need for new diagnostic approaches, for the introduction of specific therapy, which allows the adoption of prevention and control measures for these infections.
Description
Keywords
Candida Spp. Fenotipagem Genotipagem Rhodotorula Spp. Suscetibilidade