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Spheroids formation on non‐adhesive surfaces by Liquid Overlay Technique: considerations and practical approaches

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dc.contributor.authorCosta, Elisabete C.
dc.contributor.authorDiogo, Duarte Miguel de Melo
dc.contributor.authorMoreira, André
dc.contributor.authorCarvalho, Marco António Paulo de
dc.contributor.authorCorreia, Ilídio Joaquim Sobreira
dc.date.accessioned2018-03-22T09:35:23Z
dc.date.available2018-03-22T09:35:23Z
dc.date.issued2018
dc.description.abstractScalable and reproducible production of 3D cellular spheroids is highly demanded, by pharmaceutical companies, for drug screening purposes during the pre‐clinical evaluation phase. These 3D cellular constructs, unlike the monolayer culture of cells, can mimic different features of human tissues, including cellular organization, cell–cell and cell‐extracellular matrix (ECM) interactions. Up to now, different techniques (scaffold‐based and ‐free) have been used for spheroids formation, being the Liquid Overlay Technique (LOT) one of the most explored methodologies, due to its low cost and easy handling. Additionally, during the last few decades, this technique has been widely investigated in order to enhance its potential for being applied in high‐throughput analysis. Herein, an overview of the LOT advances, practical approaches, and troubleshooting is provided for those researchers that intend to produce spheroids using LOT, for drug screening purposes. Moreover, the advantages of the LOT over the other scaffold‐free techniques used for the spheroids formation are also addressed.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationCosta, E. C., Melo‐Diogo, D., Moreira, A. F., Carvalho, M. P., e Correia, I. J. (2018). “Spheroids formation on non‐adhesive surfaces by Liquid Overlay Technique: considerations and practical approaches.” Biotechnology journal, Vol. 13, pp. 1700417pt_PT
dc.identifier.doi10.1002/biot.201700417pt_PT
dc.identifier.urihttp://hdl.handle.net/10400.6/4703
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherWileypt_PT
dc.relationHealth Sciences Research Centre
dc.relationDevelopment of layer-by-layer polymeric microneedles for localized delivery of therapeutics to cervical cancer
dc.relationMULTIFUNCTIONAL GRAPHENE-OXIDE NANOCARRIERS FOR DRUG DELIVERY AND PHOTOTHERMAL THERAPY OF LUNG CANCER
dc.relation3D multicellular spheroids as high throughput platforms to screen novel combinatory therapies for treatment of pancreatic cancer
dc.relation.publisherversionhttps://onlinelibrary.wiley.com/doi/abs/10.1002/biot.201700417pt_PT
dc.subject3D cell culturept_PT
dc.subjectHigh-throughput screeningpt_PT
dc.subjectLiquid overlay techniquept_PT
dc.subjectScaffold-free techniquespt_PT
dc.subjectTumor spheroidspt_PT
dc.titleSpheroids formation on non‐adhesive surfaces by Liquid Overlay Technique: considerations and practical approachespt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleHealth Sciences Research Centre
oaire.awardTitleDevelopment of layer-by-layer polymeric microneedles for localized delivery of therapeutics to cervical cancer
oaire.awardTitleMULTIFUNCTIONAL GRAPHENE-OXIDE NANOCARRIERS FOR DRUG DELIVERY AND PHOTOTHERMAL THERAPY OF LUNG CANCER
oaire.awardTitle3D multicellular spheroids as high throughput platforms to screen novel combinatory therapies for treatment of pancreatic cancer
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UID%2FMulti%2F00709%2F2013/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/POR_CENTRO/SFRH%2FBD%2F109482%2F2015/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT//SFRH%2FBD%2F103506%2F2014/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT//SFRH%2FBD%2F103507%2F2014/PT
oaire.citation.startPage1700417pt_PT
oaire.citation.titleBiotechnology Journalpt_PT
oaire.citation.volume13pt_PT
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStreamPOR_CENTRO
person.familyNameda Rocha Costa
person.familyNamede Melo-Diogo
person.familyNameMoreira
person.familyNameCorreia
person.givenNameElisabete Cristina
person.givenNameDuarte
person.givenNameAndré Ferreira
person.givenNameIlídio Joaquim Sobreira
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person.identifier1161643
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person.identifier.ciencia-idB314-4CF0-6633
person.identifier.ciencia-id9C10-9BD8-634E
person.identifier.ciencia-id4C1E-012B-83CE
person.identifier.ciencia-idF610-7373-DC81
person.identifier.orcid0000-0002-0490-0095
person.identifier.orcid0000-0001-9984-3603
person.identifier.orcid0000-0002-0604-2506
person.identifier.orcid0000-0003-1613-9675
person.identifier.scopus-author-id55805850100
person.identifier.scopus-author-id56266511300
person.identifier.scopus-author-id56401521300
person.identifier.scopus-author-id7003557499
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.embargofctCopyright cedido à editora no momento da publicaçãopt_PT
rcaap.rightsclosedAccesspt_PT
rcaap.typearticlept_PT
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