Repository logo
 
Publication

Polyethylene glycol (PEG) molecular weight influences the ClearT2 optical clearing method for spheroids imaging by confocal laser scanning microscopy

2018Journal article Restricted access
Simple item page
dc.contributor.authorCosta, Elisabete C.
dc.contributor.authorMoreira, André
dc.contributor.authorDiogo, Duarte de Melo
dc.contributor.authorCorreia, Ilídio Joaquim Sobreira
dc.date.accessioned2018-04-18T09:38:30Z
dc.date.available2018-04-18T09:38:30Z
dc.date.issued2018
dc.description.abstractSome fluorescence microscopies, like confocal laser scanning microscopy (CLSM), have limited penetration depth. Consequently, the visualization and imaging of 3D cell cultures, such as spheroids, can be a significant challenge. Therefore, to improve the imaging of 3D tissues, clearing methods have been optimized to render transparency to the opaque spheroids. In this work, the influence of the polyethylene glycol (PEG) molecular weight (MW) used in the ClearT2 method for the imaging of PI stained spheroids was investigated. The results demonstrated that ClearT2 clearing method contribution for spheroids transparency and preservation of the PI fluorescence intensity is independent on the PEG MW. However, the ClearT2 method performed using PEG 4000 Da allowed a better PI signal penetration depth and cross-section depth. Overall, the optimization of PEG MW can improve the imaging of intact spheroids by CLSM. Further, this work may also contribute to increase the application of 3D cell culture models by pharmaceutical industry in therapeutics high-throughput screening.pt_PT
dc.description.versioninfo:eu-repo/semantics/acceptedVersionpt_PT
dc.identifier.citationCosta, E.C., Moreira, A.F., de Melo-Diogo, D., Correia, I.J. (2018) " Polyethylene glycol (PEG) molecular weight influences the ClearT2 optical clearing method for spheroids imaging by confocal laser scanning microscopy”, Journal of Biomedical Optics, accepted for publication.pt_PT
dc.identifier.urihttp://hdl.handle.net/10400.6/4740
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherSPIEpt_PT
dc.relationHealth Sciences Research Centre
dc.relationDevelopment of layer-by-layer polymeric microneedles for localized delivery of therapeutics to cervical cancer
dc.relationMULTIFUNCTIONAL GRAPHENE-OXIDE NANOCARRIERS FOR DRUG DELIVERY AND PHOTOTHERMAL THERAPY OF LUNG CANCER
dc.relation3D multicellular spheroids as high throughput platforms to screen novel combinatory therapies for treatment of pancreatic cancer
dc.subjectClearT2pt_PT
dc.subjectCLSMpt_PT
dc.subjectPEGpt_PT
dc.subjectPIpt_PT
dc.subjectSpheroidspt_PT
dc.titlePolyethylene glycol (PEG) molecular weight influences the ClearT2 optical clearing method for spheroids imaging by confocal laser scanning microscopypt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleHealth Sciences Research Centre
oaire.awardTitleDevelopment of layer-by-layer polymeric microneedles for localized delivery of therapeutics to cervical cancer
oaire.awardTitleMULTIFUNCTIONAL GRAPHENE-OXIDE NANOCARRIERS FOR DRUG DELIVERY AND PHOTOTHERMAL THERAPY OF LUNG CANCER
oaire.awardTitle3D multicellular spheroids as high throughput platforms to screen novel combinatory therapies for treatment of pancreatic cancer
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UID%2FMulti%2F00709%2F2013/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/POR_CENTRO/SFRH%2FBD%2F109482%2F2015/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT//SFRH%2FBD%2F103506%2F2014/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT//SFRH%2FBD%2F103507%2F2014/PT
oaire.citation.titleJournal of Biomedical Opticspt_PT
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStreamPOR_CENTRO
person.familyNameda Rocha Costa
person.familyNameMoreira
person.familyNamede Melo-Diogo
person.familyNameCorreia
person.givenNameElisabete Cristina
person.givenNameAndré Ferreira
person.givenNameDuarte
person.givenNameIlídio Joaquim Sobreira
person.identifier1161643
person.identifierC2z5x04AAAAJ
person.identifierUMbJ1KMAAAAJ
person.identifier.ciencia-idB314-4CF0-6633
person.identifier.ciencia-id4C1E-012B-83CE
person.identifier.ciencia-id9C10-9BD8-634E
person.identifier.ciencia-idF610-7373-DC81
person.identifier.orcid0000-0002-0490-0095
person.identifier.orcid0000-0002-0604-2506
person.identifier.orcid0000-0001-9984-3603
person.identifier.orcid0000-0003-1613-9675
person.identifier.scopus-author-id55805850100
person.identifier.scopus-author-id56401521300
person.identifier.scopus-author-id56266511300
person.identifier.scopus-author-id7003557499
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.embargofctCopyright cedido à editora no momento da publicaçãopt_PT
rcaap.rightsclosedAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublicationbfc28f1b-bd1b-4564-97e3-ae6a0c3b6c0c
relation.isAuthorOfPublicatione20bf724-86f1-4fc8-800d-39ff19b624b1
relation.isAuthorOfPublication8525f1be-ff8f-4e46-b158-e59928e8a3e5
relation.isAuthorOfPublicationf2c3e779-34a2-4309-950a-80687664c6ad
relation.isAuthorOfPublication.latestForDiscovery8525f1be-ff8f-4e46-b158-e59928e8a3e5
relation.isProjectOfPublication1bea56d9-b91c-42af-a6d6-8004792eaac0
relation.isProjectOfPublicationaf6b5d57-b2b5-4241-bd65-d80fb27f7e9a
relation.isProjectOfPublicationa61e1cb2-bd08-4ddf-806d-76b237068ee3
relation.isProjectOfPublicationf7269f43-1c1e-4809-9b13-8bf68efc42c6
relation.isProjectOfPublication.latestForDiscovery1bea56d9-b91c-42af-a6d6-8004792eaac0

Files

Original bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
2018 Costa JBO_.pdf
Size:
8.76 MB
Format:
Adobe Portable Document Format
Download
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
1.71 KB
Format:
Item-specific license agreed upon to submission
Description:
Download

Collections

FCS - DCM | Documentos por Auto-Depósito