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Authors
Abstract(s)
A microbiota vaginal é alvo de diversos estudos devido a sua diversidade de
microrganismos. Um dos microrganismos que se podem estabelecer na microbiota
vaginal é o vírus do papiloma humano (HPV – Human Papillomavirus) que se estabelece
nas células imaturas do trato genital feminino. Após a infeção pelo HPV e subsequente
evasão do sistema imune, o vírus entra na sua fase de disseminação celular e pode
persistir por meses ou até mesmo por anos podendo ocasionar lesões intraepiteliais de
baixo ou alto grau.
Com este estudo, pretendem-se avaliar os diferentes tipos de microbiota cervical e
identificar as suas características no esfregaço de material residual de citologia em meio
líquido, associando com o HPV e com Chlamydia trachomatis, Neisseria gonorrhoeae,
Mycoplasma genitalium, Mycoplasma hominis, Ureaplasma urealyticum,
Trichomonas vaginalis.
Para a realização dos ensaios, utilizou-se Xpert HPV que utiliza PCR em tempo real (RTPCR) de segunda geração para deteção de infeção por HPV de alto risco de células
escamosas. Para a identificação dos agentes do painel de IST (Infeção Sexualmente
Transmissível), realizou-se o ensaio de PCR (Polymerase Chain Reaction) em tempo real
utilizando-se o multiplex Allplex™ STI Essentials Assay. Para a confeção das lâminas,
realizou-se a técnica de Gram.
Os resultados mostram que a heterogeneidade da microbiota, a quantidade e a
capacidade de formação de biofilmes são significativamente maiores em quadros de
vaginose bacteriana. Verificou-se que a Candida sp só foi evidenciada na vaginose
bacteriana do tipo 1, sem a presença de bacilos curvos, sugestivo de Mobiluncus sp.
Verificou-se que o agente Mycoplasma hominis e o HPV de alto risco possuem a maior
percentagem quando sobreposto a presença de biofilme. O agente Mycoplasma hominis
obteve resultado de 50% quando sobreposto aos parâmetros de vaginose bacteriana do
tipo 2.
Esses resultados mostram que o HPV de alto risco pode interferir na formação da
microbiota vaginal assim a vaginose bacteriana possui relação com os agentes do painel
de IST em especial o Mycoplasma hominis que representou 65% dos resultados.
The vaginal microbiota has been widely studied due to its microorganism’s diversity. One of the microorganisms that can settle the vaginal microbiota is the Human Papilloma Virus (HPV) infecting immature cells of the female genital tract. After HPV infection and subsequent evasion of the immune system, the virus can disseminate though the cells, persisting for months or even years, leading to low or high grade intraepithelial lesions. This research aims to evaluate different microorganisms on cervical microbiota and identify its characteristics in the smear of liquid cytology residual material, associating with HPV and Chlamydia trachomatis, Neisseria gonorrhoeae, Mycoplasma genitalium, Mycoplasma hominis, Ureaplasma urealyticum, Trichomonas vaginalis. For this purpose, the second generation of real time PCR was assessed through the Xpert HPV to detect high-risk HPV infection in squamous cells. Also, the real time PCR was performed by multiplex Allplex™ STI Essentials Assay aiming to identify the agents of the STI (Sexually Transmitted Infection) panel. The Gram technique was performed to prepare the slides. The results showed that the heterogeneity of the microbiota, the amount and capacity of biofilm formation are significantly greater in bacterial vaginosis. It was identified that Candida sp was only evidenced in type 1 bacterial vaginosis without the presence of curved bacilli, suggestive of Mobiluncus sp. The Mycoplasma hominis and high-risk HPV had the highest percentage report in the presence of biofilm. And the Mycoplasma hominis reported 50% of findings when overlapping reports of type 2 bacterial vaginosis. Based on our results, the present study showed that high-risk HPV can interfere with the vaginal microbiota formation and that bacterial vaginosis has a relationship with the agents of the STI panel especially Mycoplasma hominis which representing 65% of the findings.
The vaginal microbiota has been widely studied due to its microorganism’s diversity. One of the microorganisms that can settle the vaginal microbiota is the Human Papilloma Virus (HPV) infecting immature cells of the female genital tract. After HPV infection and subsequent evasion of the immune system, the virus can disseminate though the cells, persisting for months or even years, leading to low or high grade intraepithelial lesions. This research aims to evaluate different microorganisms on cervical microbiota and identify its characteristics in the smear of liquid cytology residual material, associating with HPV and Chlamydia trachomatis, Neisseria gonorrhoeae, Mycoplasma genitalium, Mycoplasma hominis, Ureaplasma urealyticum, Trichomonas vaginalis. For this purpose, the second generation of real time PCR was assessed through the Xpert HPV to detect high-risk HPV infection in squamous cells. Also, the real time PCR was performed by multiplex Allplex™ STI Essentials Assay aiming to identify the agents of the STI (Sexually Transmitted Infection) panel. The Gram technique was performed to prepare the slides. The results showed that the heterogeneity of the microbiota, the amount and capacity of biofilm formation are significantly greater in bacterial vaginosis. It was identified that Candida sp was only evidenced in type 1 bacterial vaginosis without the presence of curved bacilli, suggestive of Mobiluncus sp. The Mycoplasma hominis and high-risk HPV had the highest percentage report in the presence of biofilm. And the Mycoplasma hominis reported 50% of findings when overlapping reports of type 2 bacterial vaginosis. Based on our results, the present study showed that high-risk HPV can interfere with the vaginal microbiota formation and that bacterial vaginosis has a relationship with the agents of the STI panel especially Mycoplasma hominis which representing 65% of the findings.
Description
Keywords
Biofilme Microbiota Papilomavírus Humano