Browsing by Issue Date, starting with "2018-07-9"
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- Design de cenários, uma perspectiva além do produtoPublication . Cervelin, Gianfrancesco Afonso; Paiva, Francisco Tiago Antunes deDiante de tantas publicidades expostas nos dias de hoje, este trabalho tem como objetivo desvendar a melhor forma para exposição de produtos, com o intuito de promoção. O enfoque do produto destacado é o de assentos de cadeiras e estofados em geral, porém as técnicas visuais contidas neste trabalho servem para quaisquer tipos de divulgação. Nesse contexto, buscou-se aprofundar em pesquisas de cenários para divulgação correta de produtos para exposições em meios físicos e virtuais. Utilizar os cenários corretos para criar maior ligação e entendimento entre consumidor e produto. Cenários para determinados objetos e público na qual ele agregue valor ao objeto divulgado, fazer do cenário parte do produto a ser divulgado, e criar cenários tão atrativos quanto o produto a ser divulgado a fim de criar ligação com quem os vê. Contudo, o design de cenários contribui muito para a promoção de produtos e uma melhor compreensão entre o espectador e o objeto divulgado.
- High Sensory Processing Sensitivity and Sexual FunctioningPublication . Nave, Carina de Fátima Rodrigues; Pereira, Henrique MarquesThis dissertation is part of the second year of the second cycle of studies in Clinical and Health Psychology. The document is then constituted by a first part that corresponds to a scientific article, subdivided into four parts - introduction, procedure, results and discussion ; and a second part that includes a theoretical annex relating to the variables under study in the scientific article, which is subdivided into four parts - a first dedicated to the variable Sensory Processing Sensitivity, a second dedicated to Sexual Functioning, a third dedicated to the relationship between Sensory Processing Sensitivity and Sexual Functioning, and a fourth where we address the Gender variable. The aim of this research is to evaluate to what extent the levels of Sensory Processing Sensitivity are related to sexual functioning, estimating the mediating role that gender could have in this relationship. We collected a sample of 1054 subjects, 34.3% were men (n=362) and 64.9% were women (n=684), with ages between 18 and 80 (M=29.4; SD=11.9). First, we applied a socio-demographic questionnaire. Then, to measure SPS we used the Highly Sensitive Person Scale (Aron & Aron, 1997) and, to assess sexual functioning, we used a Portuguese validated version of the Massachusetts General Hospital – Sexual Functioning Questionnaire (Labbate & Lare, 2001). We found that SPS and sexual functioning are statistically negatively correlated (-.093; p=.03). We also found that there are statistically significant differences in sexual functioning according to gender (t(df)=7.042; p=<.05), and according to the presence of lower or higher levels of SPS (t(df)=3.599; p<.05). Finally, we found Gender as responsible for 6.2% of the total variance in sexual functioning. This work allowed not only the acquisition of research skills, but also the extension of personal knowledge about the matter subject, and also contribute significantly to the national scientific production on an important, significant and little investigated topic at national and international level.
- Modelo tumoral in vitro para a avaliação terapêutica de fármacosPublication . Nunes, Ana Raquel Santos; Correia, Ilídio Joaquim Sobreira; Costa, Elisabete Cristina da RochaThe anticancer therapies used nowadays in the clinic display a low efficacy. The ability of cancer cells to develop resistance to drugs used in chemotherapy, contributes to the high mortality associated with this disease. The development of new drug formulations is crucial for improving patient survival rates. In vitro 2D cancer cell cultures have been the main model used, in a first phase, for the development of these new therapies. However, these cultures are unable to mimic the main characteristics of in vivo tumors, such as their drug resistance mechanisms. On the other hand, in vitro 3D cancer models, in particular the cancer cell spheroids, have a drug resistance profile similar to that found in human solid tumors, such as breast cancer. Due to that, these models have been used by researchers as a tool to study anticancer drugs. Nevertheless, the techniques and methods used to analyze the therapeutic efficacy of drugs in in vitro models are developed and standardized only for 2D cells in culture. In this way, the develop of 2D cell culture models that display a drug resistant profile similar to the spheroids can be advantageous since these models can be studied through established equipment, techniques and methodologies. Further investigations revealed that 2D cell cultures obtained by spheroid disintegration and maintained in culture medium supplemented with glutathione (GSH) had the same phenotype as the cells present in 3D spheroids. In the present work, the resistance to doxorubicin (DOX) of 2D cultures of MCF-7 obtained by disaggregation of spheroids and then cultured in culture medium supplemented with GSH was evaluated. The results obtained demonstrated that this model of cell culture presents a DOX resistance profile closer to that presented by spheroids. In fact, the 50 % inhibitory concentration (IC50) of DOX in 3D-derived MCF-7 cell cultures supplemented with GSH was about 8-times higher than that obtained for conventional 2D cell cultures. In this work it was also possible to demonstrate that the increase in resistance of spheroid-derived MCF-7 cells results from increased P-glycoprotein (P-gp) activity and from the reduction of intracellular reactive oxygen species (ROS) levels in these cells. In summary, 2D cell culture obtained from spheroid disaggregation represents an improvement for the future development of anticancer therapies, owing to its ability to present in the 2D model a drug resistance similar to that exhibited by 3D models.
- Alta Sensibilidade de Processamento Sensorial e Funcionamento SexualPublication . Aparício, Beatriz Pereira; Pereira, Henrique MarquesO presente estudo tem como principal objetivo a realização de um estudo descritivo transversal, comparativo, correlacional e inferencial, com vista a definir e testar hipóteses que relacionem as seguintes variáveis analisadas ao nível individual: Alta Sensibilidade de Processamento Sensorial; Funcionamento Sexual; Perdão-traço. Isto é, a presente dissertação mira analisar a relação existente entre a Alta Sensibilidade de Processamento Sensorial (HSP) e o Funcionamento Sexual (MGH) tendo em conta o fator mediador do Perdão-traço (PT). Para o efeito, foi utilizado o Questionário Sociodemográfico, a Escala de Alta Sensibilidade - Highly Sensitive Person Scale (Aron, 2001., traduzido para a pop. portuguesa por Pereira et. al, 2017), o Questionário de Funcionamento Sexual (MGH-SFQ – Labbate & Lare, 2001; Pereira, 2017) e o Questionário de Perdão-traço (TFS - Berry, Worthington, O´Connor, Parrott & Wade 2005). Estes instrumentos de avaliação foram aplicados a uma amostra de 1063 indivíduos (n=1063), 364 do género masculino (H) (34.2%) e 691 do género feminino (F) (65.0%), com idades iguais ou superiores a 18 anos de idade (a média de idade obtida foi de 29 anos de idade (M= 29.27)). De uma forma geral, os resultados apontam para a existência de uma correlação negativa entre a variável HSP e a variável MGH. De igual forma, a variável HSP correlaciona-se negativamente com a variável PT. Já a variável MGH parece correlacionar-se de forma positiva com a variável PT. O presente estudo finaliza com uma reflexão sobre as implicações práticas da relação existente entre as variáveis em estudo, apresentando ainda algumas limitações e sugestões para futuras investigações nesta temática.
- Development of a new bioactive membrane to be used for the treatment of skin injuriesPublication . Simões, Déborah; Correia, Ilídio Joaquim Sobreira; Miguel, Sónia Alexandra PereiraSkin and soft tissue infections (SSTIs) have high rates of morbidity and mortality associated. Despite the successful treatment of some SSTIs, those affecting the subcutaneous tissue, fascia, or muscle delay the healing process and can lead to life-threatening conditions. Therefore, it is fundamental to develop antimicrobial wound dressings to deal with such pathological situations. Recently, wound dressings loaded with antimicrobial agents emerged as viable options to reduce wound bacterial colonization and infection, in order to improve the healing process. In this thesis, a new antimicrobial wound dressing was produced and characterized. In this context, polycaprolactone (PCL) nanofibrous membranes functionalized with biosynthesized maillard reaction products (MRPs) were produced using an electrospinning apparatus. The functionalization of PCL nanofibers with MRPs allowed the production of membranes with the mechanical, wettability and porosity features required for wound exudate absorption as well as nutrients and gas exchange. Furthermore, MRPs-modified PCL membranes were also able to inhibit Staphylococcus aureus and Pseudomonas aeruginosa growth, without inducing any cytotoxic effect to human fibroblast cells. These findings support the potential use of the produced membranes in the healing process.